Biochemical Pathways and Environmental Responses in Plants, Part A, Volume 676 in the Methods in Enzymology series highlights new advances in the field with this new volume presenting interesting chapters on topics such as Structure, function, and engineering of plant polyketide synthases, A sensitive LC-MS/MS assay for enzymatic characterization of methylthioalkylmalate synthase involved in glucosinolate side-chain elongation, Assaying formate-tetrahydrofolate ligase with monoglutamylated and polyglutamylated substrates using a fluorescence-HPLC based assay, An Approach to Nearest Neighbor Analysis of Pigmented Protein Complexes by Using Chemical Crosslinking in Combination with Mass Spectrometry, and much more.
Other chapters cover Biochemical characterization of plant aromatic aminotransferases, Functional Analysis of Phosphoethanolamine N-methyltransferase (PMT) in Plants and Parasites, A structure-guided computational screening approach for predicting plant enzyme-metabolite interactions, Plant metacaspase: an example of microcrystal structure determination and analysis, Biocatalytic system for comparative assessment of functional association of cytochrome P450 monooxygenases with their redox partners, Dirigent Protein Family Function and Structure, and more.
1. Cover image - p. Cover 2. Table of Contents - p. iii 3. Series Page - p. iii 4. Copyright - p. iii 5. Contributors - p. iii 6. Preface - p. xv 7. Section I: Protein - p. xvi 8. Chapter One: Structure, function, and engineering of plant polyketide synthases - p. 3 9. Chapter Two: An LC-MS/MS assay for enzymatic characterization of methylthioalkylmalate synthase (MAMS) involved in glucosinolate biosynthesis - p. 49 10. Chapter Three: A structure-guided computational screening approach for predicting plant enzyme–metabolite interactions - p. 79 11. Chapter Four: Plant metacaspase: A case study of microcrystal structure determination and analysis - p. 103 12. Chapter Five: Biocatalytic system for comparatively assessing the functional association of monolignol cytochrome P450 monooxygenases with their redox partners - p. 133 13. Chapter Six: Programmable chemical actuator control of soluble and membrane-bound enzymatic catalysis - p. 159 14. Section II: Metabolite - p. 194 15. Chapter Seven: Measurement of flux through sulfate assimilation using [35S]sulfate - p. 197 16. Chapter Eight: Isoprene measurements to assess plant hydrocarbon emissions and the methylerythritol pathway - p. 211 17. Chapter Nine: Using targeted metabolomics to elucidate the indole auxin network in plants - p. 250 18. Chapter Ten: Tag you're it: Application of stable isotope labeling and LC-MS to identify the precursors of specialized metabolites in plants - p. 288 19. Section III: Environment - p. 303 20. Chapter Eleven: Modification of G-protein biochemistry and its effect on plant/environment interaction - p. 307 21. Chapter Twelve: Soybean seed proteomics: Methods for the isolation, detection, and identification of low abundance proteins - p. 325 22. Chapter Thirteen: Proteomics and phosphoproteomics of C3 to CAM transition in the common ice plant - p. 347 23. Chapter Fourteen: Identification of MPK4 kinase interactome using TurboID proximity labeling proteomics in Arabidopsis thaliana - p. 369 24. Chapter Fifteen: Detection of protein persulfidation in plants by the dimedone switch method - p. 385 25. Chapter Sixteen: CRISPR-Cas mediated genome engineering of cyanobacteria - p. 406
Biochemical Pathways and Environmental Responses in Plants, Part A, Volume 676 in the Methods in Enzymology series highlights new advances in the field with this new volume presenting interesting chapters on topics such as Structure, function, and engineering of plant polyketide synthases, A sensitive LC-MS/MS assay for enzymatic characterization of methylthioalkylmalate synthase involved in glucosinolate side-chain elongation, Assaying formate-tetrahydrofolate ligase with monoglutamylated and polyglutamylated substrates using a fluorescence-HPLC based assay, An Approach to Nearest Neighbor Analysis of Pigmented Protein Complexes by Using Chemical Crosslinking in Combination with Mass Spectrometry, and much more.
Other chapters cover Biochemical characterization of plant aromatic aminotransferases, Functional Analysis of Phosphoethanolamine N-methyltransferase (PMT) in Plants and Parasites, A structure-guided computational screening approach for predicting plant enzyme-metabolite interactions, Plant metacaspase: an example of microcrystal structure determination and analysis, Biocatalytic system for comparative assessment of functional association of cytochrome P450 monooxygenases with their redox partners, Dirigent Protein Family Function and Structure, and more.
1. Cover image - p. Cover 2. Table of Contents - p. iii 3. Series Page - p. iii 4. Copyright - p. iii 5. Contributors - p. iii 6. Preface - p. xv 7. Section I: Protein - p. xvi 8. Chapter One: Structure, function, and engineering of plant polyketide synthases - p. 3 9. Chapter Two: An LC-MS/MS assay for enzymatic characterization of methylthioalkylmalate synthase (MAMS) involved in glucosinolate biosynthesis - p. 49 10. Chapter Three: A structure-guided computational screening approach for predicting plant enzyme–metabolite interactions - p. 79 11. Chapter Four: Plant metacaspase: A case study of microcrystal structure determination and analysis - p. 103 12. Chapter Five: Biocatalytic system for comparatively assessing the functional association of monolignol cytochrome P450 monooxygenases with their redox partners - p. 133 13. Chapter Six: Programmable chemical actuator control of soluble and membrane-bound enzymatic catalysis - p. 159 14. Section II: Metabolite - p. 194 15. Chapter Seven: Measurement of flux through sulfate assimilation using [35S]sulfate - p. 197 16. Chapter Eight: Isoprene measurements to assess plant hydrocarbon emissions and the methylerythritol pathway - p. 211 17. Chapter Nine: Using targeted metabolomics to elucidate the indole auxin network in plants - p. 250 18. Chapter Ten: Tag you're it: Application of stable isotope labeling and LC-MS to identify the precursors of specialized metabolites in plants - p. 288 19. Section III: Environment - p. 303 20. Chapter Eleven: Modification of G-protein biochemistry and its effect on plant/environment interaction - p. 307 21. Chapter Twelve: Soybean seed proteomics: Methods for the isolation, detection, and identification of low abundance proteins - p. 325 22. Chapter Thirteen: Proteomics and phosphoproteomics of C3 to CAM transition in the common ice plant - p. 347 23. Chapter Fourteen: Identification of MPK4 kinase interactome using TurboID proximity labeling proteomics in Arabidopsis thaliana - p. 369 24. Chapter Fifteen: Detection of protein persulfidation in plants by the dimedone switch method - p. 385 25. Chapter Sixteen: CRISPR-Cas mediated genome engineering of cyanobacteria - p. 406
