Biological NMR, Part A, the latest release in the Methods of Enzymology series, highlights new advances in the field, with this new volume presenting interesting chapters on topics such as Protein methyl labeling, Membrane protein expression – yeast, Protein aromatic labeling, His-tag/Metal contamination, Bicelles, nanodiscs and micelles MP host, PTM – phosphorylation, PTM – lipidation, Screening platform for receptor-ligand discovery
Solution Spectroscopy, Large protein strategies, NUS data collection/analysis, F19 incl. hydration, ODNP - hydration, Reverse micelle - Hydration Solid State Spectroscopy, SS NMR membrane proteins, SS NMR soluble/aggregate proteins, SS DNP - general, SS NMR nucleic acids, Structure determination and computer analysis, and much more.
1. Cover image - p. iv 2. Title page - p. iv 3. Table of Contents - p. iv 4. Copyright - p. iv 5. Contributors - p. xi 6. Preface - p. xv 7. Chapter One: Selective Methyl Labeling of Proteins: Enabling Structural and Mechanistic Studies As Well As Drug Discovery Applications by Solution-State NMR - p. xvii 8. Chapter Two: Isotopic Labeling of Eukaryotic Membrane Proteins for NMR Studies of Interactions and Dynamics - p. 36 9. Chapter Three: Optimal Isotope Labeling of Aromatic Amino Acid Side Chains for NMR Studies of Protein Dynamics - p. 65 10. Chapter Four: A Quick and Colorful Method to Measure Low-Level Contaminations of Paramagnetic Ni2+ in Protein Samples Purified by Immobilized Metal Ion Affinity Chromatography - p. 86 11. Chapter Five: Toolkit for NMR Studies of Methyl-Labeled Proteins - p. 106 12. Chapter Six: Synthetic Biology-Based Solution NMR Studies on Membrane Proteins in Lipid Environments - p. 142 13. Chapter Seven: Preparation of Phosphorylated Proteins for NMR Spectroscopy - p. 185 14. Chapter Eight: Synthesis and NMR Characterization of the Prenylated Peptide, a-Factor - p. 205 15. Chapter Nine: The Preparation and Solution NMR Spectroscopy of Human Glycoproteins Is Accessible and Rewarding - p. 238 16. Chapter Ten: Nonuniform Sampling for NMR Spectroscopy - p. 261 17. Chapter Eleven: Exploring Protein Conformational Landscapes Using High-Pressure NMR - p. 291 18. Chapter Twelve: CS-ROSETTA - p. 320 19. Chapter Thirteen: Combining Evolutionary Covariance and NMR Data for Protein Structure Determination - p. 362 20. Chapter Fourteen: Combining NMR Spectroscopy and Molecular Dynamic Simulations to Solve and Analyze the Structure of Protein–RNA Complexes - p. 392
Biological NMR, Part A, the latest release in the Methods of Enzymology series, highlights new advances in the field, with this new volume presenting interesting chapters on topics such as Protein methyl labeling, Membrane protein expression – yeast, Protein aromatic labeling, His-tag/Metal contamination, Bicelles, nanodiscs and micelles MP host, PTM – phosphorylation, PTM – lipidation, Screening platform for receptor-ligand discovery
Solution Spectroscopy, Large protein strategies, NUS data collection/analysis, F19 incl. hydration, ODNP - hydration, Reverse micelle - Hydration Solid State Spectroscopy, SS NMR membrane proteins, SS NMR soluble/aggregate proteins, SS DNP - general, SS NMR nucleic acids, Structure determination and computer analysis, and much more.
1. Cover image - p. iv 2. Title page - p. iv 3. Table of Contents - p. iv 4. Copyright - p. iv 5. Contributors - p. xi 6. Preface - p. xv 7. Chapter One: Selective Methyl Labeling of Proteins: Enabling Structural and Mechanistic Studies As Well As Drug Discovery Applications by Solution-State NMR - p. xvii 8. Chapter Two: Isotopic Labeling of Eukaryotic Membrane Proteins for NMR Studies of Interactions and Dynamics - p. 36 9. Chapter Three: Optimal Isotope Labeling of Aromatic Amino Acid Side Chains for NMR Studies of Protein Dynamics - p. 65 10. Chapter Four: A Quick and Colorful Method to Measure Low-Level Contaminations of Paramagnetic Ni2+ in Protein Samples Purified by Immobilized Metal Ion Affinity Chromatography - p. 86 11. Chapter Five: Toolkit for NMR Studies of Methyl-Labeled Proteins - p. 106 12. Chapter Six: Synthetic Biology-Based Solution NMR Studies on Membrane Proteins in Lipid Environments - p. 142 13. Chapter Seven: Preparation of Phosphorylated Proteins for NMR Spectroscopy - p. 185 14. Chapter Eight: Synthesis and NMR Characterization of the Prenylated Peptide, a-Factor - p. 205 15. Chapter Nine: The Preparation and Solution NMR Spectroscopy of Human Glycoproteins Is Accessible and Rewarding - p. 238 16. Chapter Ten: Nonuniform Sampling for NMR Spectroscopy - p. 261 17. Chapter Eleven: Exploring Protein Conformational Landscapes Using High-Pressure NMR - p. 291 18. Chapter Twelve: CS-ROSETTA - p. 320 19. Chapter Thirteen: Combining Evolutionary Covariance and NMR Data for Protein Structure Determination - p. 362 20. Chapter Fourteen: Combining NMR Spectroscopy and Molecular Dynamic Simulations to Solve and Analyze the Structure of Protein–RNA Complexes - p. 392
