Biomolecular Interactions: Part A, Volume 169, the latest release in the Methods in Cell Biology series, highlights new advances in the field, with this new volume presenting interesting chapters on a variety of timely topics, including Emerging Mechanisms of Targeted Protein Degradation by Molecular Glues, Design and use of programmable DNA Hydrogels, Oligomerization of membrane receptors: Approaches to measure in live cells, Interactions of alpha-synuclein with biomolecules, Gel-electrophoresis based method for biomolecular interaction, Recombinant centrosome expression in bacterial system, Reconstituting CCL5-CCR5 complex for structural and mechanistic analysis, Protein engineering and design in ion channel receptors, and much more.
1. Cover image - p. i 2. Table of Contents - p. iii 3. Series Page - p. iii 4. Copyright - p. iii 5. Contributors - p. iii 6. Preface - p. iii 7. Chapter 1: Emerging mechanisms of targeted protein degradation by molecular glues - p. 1 8. Chapter 2: Visualizing G protein-coupled receptor homomers using photoactivatable dye localization microscopy - p. 27 9. Chapter 3: Interaction of alpha-synuclein with lipids - p. 43 10. Chapter 4: Gel-electrophoresis based method for biomolecular interaction - p. 67 11. Chapter 5: Yeast 2-hybrid assay for investigating the interaction between the centrosome proteins PLK4 and STIL - p. 108 12. Chapter 6: Preparation of a stable CCL5·CCR5·Gi signaling complex for Cryo-EM analysis - p. 115 13. Chapter 7: Protein engineering and design in ion channels and receptors - p. 143 14. Chapter 8: Discovery of Virus-Host interactions using bioinformatic tools - p. 173 15. Chapter 9: Best practices for resilient automation of biomolecular simulations - p. 199 16. Chapter 10: Label-free impedance measurements to unravel biomolecular interactions involved in G protein-coupled receptor signaling - p. 221 17. Chapter 11: Proximity labeling for investigating protein-protein interactions - p. 237 18. Chapter 12: An intrabody sensor to monitor conformational activation of β-arrestins - p. 267 19. Chapter 13: Nanoluciferase-based methods to monitor activation, modulation and trafficking of atypical chemokine receptors - p. 279 20. Chapter 14: In-cellulo chemical cross-linking to visualize protein-protein interactions - p. 295 21. Chapter 15: Nanoluciferase-based complementation assay for systematic profiling of GPCR–GRK interactions - p. 309 22. Chapter 16: DNA hydrogels: Principles, synthesis, characterization and applications to cell biology - p. 323
Biomolecular Interactions: Part A, Volume 169, the latest release in the Methods in Cell Biology series, highlights new advances in the field, with this new volume presenting interesting chapters on a variety of timely topics, including Emerging Mechanisms of Targeted Protein Degradation by Molecular Glues, Design and use of programmable DNA Hydrogels, Oligomerization of membrane receptors: Approaches to measure in live cells, Interactions of alpha-synuclein with biomolecules, Gel-electrophoresis based method for biomolecular interaction, Recombinant centrosome expression in bacterial system, Reconstituting CCL5-CCR5 complex for structural and mechanistic analysis, Protein engineering and design in ion channel receptors, and much more.
1. Cover image - p. i 2. Table of Contents - p. iii 3. Series Page - p. iii 4. Copyright - p. iii 5. Contributors - p. iii 6. Preface - p. iii 7. Chapter 1: Emerging mechanisms of targeted protein degradation by molecular glues - p. 1 8. Chapter 2: Visualizing G protein-coupled receptor homomers using photoactivatable dye localization microscopy - p. 27 9. Chapter 3: Interaction of alpha-synuclein with lipids - p. 43 10. Chapter 4: Gel-electrophoresis based method for biomolecular interaction - p. 67 11. Chapter 5: Yeast 2-hybrid assay for investigating the interaction between the centrosome proteins PLK4 and STIL - p. 108 12. Chapter 6: Preparation of a stable CCL5·CCR5·Gi signaling complex for Cryo-EM analysis - p. 115 13. Chapter 7: Protein engineering and design in ion channels and receptors - p. 143 14. Chapter 8: Discovery of Virus-Host interactions using bioinformatic tools - p. 173 15. Chapter 9: Best practices for resilient automation of biomolecular simulations - p. 199 16. Chapter 10: Label-free impedance measurements to unravel biomolecular interactions involved in G protein-coupled receptor signaling - p. 221 17. Chapter 11: Proximity labeling for investigating protein-protein interactions - p. 237 18. Chapter 12: An intrabody sensor to monitor conformational activation of β-arrestins - p. 267 19. Chapter 13: Nanoluciferase-based methods to monitor activation, modulation and trafficking of atypical chemokine receptors - p. 279 20. Chapter 14: In-cellulo chemical cross-linking to visualize protein-protein interactions - p. 295 21. Chapter 15: Nanoluciferase-based complementation assay for systematic profiling of GPCR–GRK interactions - p. 309 22. Chapter 16: DNA hydrogels: Principles, synthesis, characterization and applications to cell biology - p. 323
