Enzyme Reprogramming, Volume 719 in the Methods in Enzymology series, highlights new advances in the field, with this new volume presenting interesting chapters on topics such as Practical Considerations for Reaction Development with Vanadium-Dependent Haloperoxidases, Characterization and Engineering of thermostable PLP-dependent alpha-oxoamine synthases (AOSs); versatile C-C bond forming biocatalysts, Thiamine diphosphate Enzymes for Photobiocatalytic Enantioselective C(sp3)–H Acylation, Engineering a PLP-dependent Mannichase, Transferase engineering for SAM analog synthesis using combinatorial library design and high-throughput screening, Biaryl Coupling reactions catalyzed by Cytochrome P450s, and much more.
Other chapters cover Engineering of a-ketoglutarate-dependent amino acid hydroxylases, Repurposing thiamine-dependent enzymes for radical biocatalysis, Directed evolution of nonheme Fe Enzymes for azidation, Directed evolution of P450 dearomatases for catalytic asymmetric radical dearomatization, Genetically encoded artificial photoenzymes for enantioselective [2+2] photocycloadditions, Unmasking the Reverse Activity of Old Yellow Enzymes for Selective Carbonyl Desaturation, and more.
1. Title of Book - p. i 2. Cover image - p. i 3. Title page - p. ii 4. Table of Contents - p. iii 5. Series Page - p. iii 6. Copyright - p. iv 7. Contributors - p. xiii 8. Chapter One: Biaryl coupling reactions catalyzed by cytochrome P450s - p. 1 9. Chapter Two: A metallophotoredox strategy for biocatalytic radical C–N3 and C–SCN bond formation - p. 19 10. Chapter Three: Efficient transferase engineering for SAM analog synthesis using combinatorial library design and high-throughput LC/MS screening - p. 39 11. Chapter Four: Old yellow enzyme-catalyzed desymmetrizing desaturation - p. 71 12. Chapter Five: Characterisation and engineering of thermostable PLP-dependent alpha-oxoamine synthases (AOSs); versatile C-C bond forming biocatalysts - p. 93 13. Chapter Six: Engineering a PLP-dependent Mannichase - p. 121 14. Chapter Seven: Repurposing branched chain amino acid aminotransferase in the chemoenzymatic synthesis of azacyclic noncanonical amino acids - p. 135 15. Chapter Eight: Threonine aldolase-catalyzed stereoselective radical α-alkylation of amino acids - p. 147 16. Chapter Nine: Repurposing thiamine-dependent enzymes for radical biocatalysis - p. 169 17. Chapter Ten: Photobiocatalytic enantioselective C(sp³)–H acylation using thiamine-dependent enzymes - p. 191 18. Chapter Eleven: Genetically encoded artificial photoenzymes for enantioselective [2 + 2] photocycloadditions - p. 211
Enzyme Reprogramming, Volume 719 in the Methods in Enzymology series, highlights new advances in the field, with this new volume presenting interesting chapters on topics such as Practical Considerations for Reaction Development with Vanadium-Dependent Haloperoxidases, Characterization and Engineering of thermostable PLP-dependent alpha-oxoamine synthases (AOSs); versatile C-C bond forming biocatalysts, Thiamine diphosphate Enzymes for Photobiocatalytic Enantioselective C(sp3)–H Acylation, Engineering a PLP-dependent Mannichase, Transferase engineering for SAM analog synthesis using combinatorial library design and high-throughput screening, Biaryl Coupling reactions catalyzed by Cytochrome P450s, and much more.
Other chapters cover Engineering of a-ketoglutarate-dependent amino acid hydroxylases, Repurposing thiamine-dependent enzymes for radical biocatalysis, Directed evolution of nonheme Fe Enzymes for azidation, Directed evolution of P450 dearomatases for catalytic asymmetric radical dearomatization, Genetically encoded artificial photoenzymes for enantioselective [2+2] photocycloadditions, Unmasking the Reverse Activity of Old Yellow Enzymes for Selective Carbonyl Desaturation, and more.
1. Title of Book - p. i 2. Cover image - p. i 3. Title page - p. ii 4. Table of Contents - p. iii 5. Series Page - p. iii 6. Copyright - p. iv 7. Contributors - p. xiii 8. Chapter One: Biaryl coupling reactions catalyzed by cytochrome P450s - p. 1 9. Chapter Two: A metallophotoredox strategy for biocatalytic radical C–N3 and C–SCN bond formation - p. 19 10. Chapter Three: Efficient transferase engineering for SAM analog synthesis using combinatorial library design and high-throughput LC/MS screening - p. 39 11. Chapter Four: Old yellow enzyme-catalyzed desymmetrizing desaturation - p. 71 12. Chapter Five: Characterisation and engineering of thermostable PLP-dependent alpha-oxoamine synthases (AOSs); versatile C-C bond forming biocatalysts - p. 93 13. Chapter Six: Engineering a PLP-dependent Mannichase - p. 121 14. Chapter Seven: Repurposing branched chain amino acid aminotransferase in the chemoenzymatic synthesis of azacyclic noncanonical amino acids - p. 135 15. Chapter Eight: Threonine aldolase-catalyzed stereoselective radical α-alkylation of amino acids - p. 147 16. Chapter Nine: Repurposing thiamine-dependent enzymes for radical biocatalysis - p. 169 17. Chapter Ten: Photobiocatalytic enantioselective C(sp³)–H acylation using thiamine-dependent enzymes - p. 191 18. Chapter Eleven: Genetically encoded artificial photoenzymes for enantioselective [2 + 2] photocycloadditions - p. 211
