Genetic Engineering and Genome Editing for Zinc Biofortification of Rice provides the first single-volume, comprehensive resource on genetic engineering approaches, including novel genome editing techniques, that are carried out in rice, a staple crop for much of the world’s population. Dietary zinc deficiency can lead to negative health outcomes, including increased risk of stunting, respiratory diseases, diarrhea, mortality during childhood, and preterm births in pregnancy. By providing a complete view of the need for zinc biofortification in rice, sections in this book discuss state-of-the-art scientific advances, and then go further, placing them in their proper scientific, regulatory and socioeconomic contexts.While zinc biofortification can be achieved through conventional breeding, genetic engineering and agronomic practices, this is the first reference to bring all the latest insights and understanding to a comprehensive resource that is based on real-world experience and targeted applications.
1. Title of Book - p. i 2. Cover image - p. i 3. Title page - p. ii 4. Table of Contents - p. iii 5. Copyright - p. iii 6. List of contributors - p. ix 7. Foreword - p. xi 8. Preface - p. xiii 9. Chapter 1. Molecular mechanisms leading to grain Zn accumulation in rice - p. 1 10. Chapter 2. Molecular links between iron and zinc biofortification in rice - p. 15 11. Chapter 3. Ionomics-based imaging, localization and quantification of zinc and other micronutrients in rice grains for biofortification research - p. 31 12. Chapter 4. Recent advances in precise plant genome editing technology - p. 45 13. Chapter 5. Practical protocol for design and construction of a transformation vector for prime editing in rice - p. 55 14. Chapter 6. Stages of development of genetically modified (GM) plants - p. 69 15. Chapter 7. Nicotianamine enhances zinc transport to seeds for biofortification - p. 77 16. Chapter 8. Zinc biofortification of rice by engineering metal transporter genes - p. 97 17. Chapter 9. Reducing cadmium content in zinc biofortified rice through genetic manipulation - p. 115 18. Chapter 10. Improving bioavailability of zinc in rice grains by reducing antinutrients through genetic engineering - p. 131 19. Chapter 11. Leveraging intellectual property for the development of genome edited crops by public research institutes - p. 143 20. Chapter 12. Prospects and challenges associated with GM biofortified crops - p. 153 21. Chapter 13. Pathway from laboratory to market: regulatory approval process during the development of genetically modified crops—Philippine perspective - p. 167 22. Chapter 14. Economic feasibility of genetically modified zinc-fortified rice - p. 183 23. Index - p. 199
Genetic Engineering and Genome Editing for Zinc Biofortification of Rice provides the first single-volume, comprehensive resource on genetic engineering approaches, including novel genome editing techniques, that are carried out in rice, a staple crop for much of the world’s population. Dietary zinc deficiency can lead to negative health outcomes, including increased risk of stunting, respiratory diseases, diarrhea, mortality during childhood, and preterm births in pregnancy. By providing a complete view of the need for zinc biofortification in rice, sections in this book discuss state-of-the-art scientific advances, and then go further, placing them in their proper scientific, regulatory and socioeconomic contexts.While zinc biofortification can be achieved through conventional breeding, genetic engineering and agronomic practices, this is the first reference to bring all the latest insights and understanding to a comprehensive resource that is based on real-world experience and targeted applications.
1. Title of Book - p. i 2. Cover image - p. i 3. Title page - p. ii 4. Table of Contents - p. iii 5. Copyright - p. iii 6. List of contributors - p. ix 7. Foreword - p. xi 8. Preface - p. xiii 9. Chapter 1. Molecular mechanisms leading to grain Zn accumulation in rice - p. 1 10. Chapter 2. Molecular links between iron and zinc biofortification in rice - p. 15 11. Chapter 3. Ionomics-based imaging, localization and quantification of zinc and other micronutrients in rice grains for biofortification research - p. 31 12. Chapter 4. Recent advances in precise plant genome editing technology - p. 45 13. Chapter 5. Practical protocol for design and construction of a transformation vector for prime editing in rice - p. 55 14. Chapter 6. Stages of development of genetically modified (GM) plants - p. 69 15. Chapter 7. Nicotianamine enhances zinc transport to seeds for biofortification - p. 77 16. Chapter 8. Zinc biofortification of rice by engineering metal transporter genes - p. 97 17. Chapter 9. Reducing cadmium content in zinc biofortified rice through genetic manipulation - p. 115 18. Chapter 10. Improving bioavailability of zinc in rice grains by reducing antinutrients through genetic engineering - p. 131 19. Chapter 11. Leveraging intellectual property for the development of genome edited crops by public research institutes - p. 143 20. Chapter 12. Prospects and challenges associated with GM biofortified crops - p. 153 21. Chapter 13. Pathway from laboratory to market: regulatory approval process during the development of genetically modified crops—Philippine perspective - p. 167 22. Chapter 14. Economic feasibility of genetically modified zinc-fortified rice - p. 183 23. Index - p. 199
