Microbial Cell Factories Engineering for Production of Biomolecules presents a compilation of chapters written by eminent scientists worldwide. Sections cover major tools and technologies for DNA synthesis, design of biosynthetic pathways, synthetic biology tools, biosensors, cell-free systems, computer-aided design, OMICS tools, CRISPR/Cas systems, and many more. Although it is not easy to find relevant information collated in a single volume, the book covers the production of a wide range of biomolecules from several MCFs, including Escherichia coli, Bacillus subtilis, Pseudomonas putida, Streptomyces, Corynebacterium, Cyanobacteria, Saccharomyces cerevisiae, Pichia pastoris and Yarrowia lipolytica, and algae, among many others.
This will be an excellent platform from which scientific knowledge can grow and widen in MCF engineering research for the production of biomolecules. Needless to say, the book is a valuable source of information not only for researchers designing cell factories, but also for students, metabolic engineers, synthetic biologists, genome engineers, industrialists, stakeholders and policymakers interested in harnessing the potential of MCFs in several fields.
1. Chapter 1 An introduction to microbial cell factories for production of biomolecules - p. 1 2. Chapter 2 Advances in long DNA synthesis - p. 21 3. Chapter 3 Discovery of enzymes responsible for cyclization and postmodification in triterpenoid biosynthesis - p. 37 4. Chapter 4 Synthetic biology approaches for secondary metabolism engineering - p. 51 5. Chapter 5 Synthetic biology design tools for metabolic engineering - p. 65 6. Chapter 6 Metabolic engineering for microbial cell factories - p. 79 7. Chapter 7 CRISPR-based tools for microbial cell factories - p. 95 8. Chapter 8 Escherichia coli, the workhorse cell factory for the production of chemicals - p. 115 9. Chapter 9 Bacillus subtilis-based microbial cell factories - p. 139 10. Chapter 10 Pseudomonas putida–based cell factories - p. 165 11. Chapter 11 Streptomyces-based cell factories for production of biomolecules and bioactive metabolites - p. 183 12. Chapter 12 Corynebacterium glutamicum as a robust microbial factory for production of value-added proteins and small molecules: fundamentals and applications - p. 235 13. Chapter 13 Production of high value-added chemicals by engineering methylotrophic cell factories - p. 265 14. Chapter 14 Cyanobacteria-based microbial cell factories for production of industrial products - p. 277 15. Chapter 15 Integrated omics perspective to understand the production of high-value added biomolecules (HVABs) in microalgal cell factories - p. 303 16. Chapter 16 Saccharomyces cerevisiae as a microbial cell factory - p. 319 17. Chapter 17 Pichia pastoris-based microbial cell factories - p. 335 18. Chapter 18 Yarrowia lipolytica engineering as a source of microbial cell factories - p. 345 19. Chapter 19 Engineering of microbial cell factories for production of plant-based natural products - p. 381 20. Chapter 20 Engineering of microbial cell factories for omics-guided production of medically important biomolecules - p. 393 21. Chapter 21 Advances and applications of cell-free systems for metabolic production - p. 407 22. Chapter 22 Microbial biosensors for discovery and engineering of enzymes and metabolism - p. 421 23. Chapter 23 Manipulation of global regulators in Escherichia coli for the synthesis of biotechnologically relevant products - p. 437 24. Index - p. 455
Microbial Cell Factories Engineering for Production of Biomolecules presents a compilation of chapters written by eminent scientists worldwide. Sections cover major tools and technologies for DNA synthesis, design of biosynthetic pathways, synthetic biology tools, biosensors, cell-free systems, computer-aided design, OMICS tools, CRISPR/Cas systems, and many more. Although it is not easy to find relevant information collated in a single volume, the book covers the production of a wide range of biomolecules from several MCFs, including Escherichia coli, Bacillus subtilis, Pseudomonas putida, Streptomyces, Corynebacterium, Cyanobacteria, Saccharomyces cerevisiae, Pichia pastoris and Yarrowia lipolytica, and algae, among many others.
This will be an excellent platform from which scientific knowledge can grow and widen in MCF engineering research for the production of biomolecules. Needless to say, the book is a valuable source of information not only for researchers designing cell factories, but also for students, metabolic engineers, synthetic biologists, genome engineers, industrialists, stakeholders and policymakers interested in harnessing the potential of MCFs in several fields.
1. Chapter 1 An introduction to microbial cell factories for production of biomolecules - p. 1 2. Chapter 2 Advances in long DNA synthesis - p. 21 3. Chapter 3 Discovery of enzymes responsible for cyclization and postmodification in triterpenoid biosynthesis - p. 37 4. Chapter 4 Synthetic biology approaches for secondary metabolism engineering - p. 51 5. Chapter 5 Synthetic biology design tools for metabolic engineering - p. 65 6. Chapter 6 Metabolic engineering for microbial cell factories - p. 79 7. Chapter 7 CRISPR-based tools for microbial cell factories - p. 95 8. Chapter 8 Escherichia coli, the workhorse cell factory for the production of chemicals - p. 115 9. Chapter 9 Bacillus subtilis-based microbial cell factories - p. 139 10. Chapter 10 Pseudomonas putida–based cell factories - p. 165 11. Chapter 11 Streptomyces-based cell factories for production of biomolecules and bioactive metabolites - p. 183 12. Chapter 12 Corynebacterium glutamicum as a robust microbial factory for production of value-added proteins and small molecules: fundamentals and applications - p. 235 13. Chapter 13 Production of high value-added chemicals by engineering methylotrophic cell factories - p. 265 14. Chapter 14 Cyanobacteria-based microbial cell factories for production of industrial products - p. 277 15. Chapter 15 Integrated omics perspective to understand the production of high-value added biomolecules (HVABs) in microalgal cell factories - p. 303 16. Chapter 16 Saccharomyces cerevisiae as a microbial cell factory - p. 319 17. Chapter 17 Pichia pastoris-based microbial cell factories - p. 335 18. Chapter 18 Yarrowia lipolytica engineering as a source of microbial cell factories - p. 345 19. Chapter 19 Engineering of microbial cell factories for production of plant-based natural products - p. 381 20. Chapter 20 Engineering of microbial cell factories for omics-guided production of medically important biomolecules - p. 393 21. Chapter 21 Advances and applications of cell-free systems for metabolic production - p. 407 22. Chapter 22 Microbial biosensors for discovery and engineering of enzymes and metabolism - p. 421 23. Chapter 23 Manipulation of global regulators in Escherichia coli for the synthesis of biotechnologically relevant products - p. 437 24. Index - p. 455
