Mitochondrial Translocases Part A series, highlights new advances in the field, with this new volume presenting interesting chapters. Each chapter is written by an international board of authors.
1. Cover image - p. i 2. Title page - p. ii 3. Table of Contents - p. iii 4. Series Page - p. iii 5. Copyright - p. iii 6. Contributors - p. iii 7. Preface - p. iii 8. Section 1 Isolation of mitochondria - p. p27 9. Chapter One: Isolation of yeast mitochondria by differential centrifugation - p. 4 10. Chapter Two: Isolation of yeast mitochondria by affinity purification using magnetic beads - p. 21 11. Chapter Three: A modified procedure for separating yeast peroxisomes from mitochondria - p. 38 12. Section 2 Analysis of mitochondrial proteins - p. 55 13. Chapter Four: Subcellular fractionation by differential centrifugation for mitochondrial studies - p. 62 14. Chapter Five: Monitoring mitochondrial localization of dual localized proteins using a Bi-Genomic Mitochondrial-Split-GFP - p. 76 15. Chapter Six: Analysis of mitochondrial biogenesis and protein localization by genetic screens and automated imaging - p. 98 16. Chapter Seven: Methods to monitor mitochondrial disulfide bonds - p. 126 17. Section 3 Prediction, analysis and processing of mitochondrial targeting signals - p. 156 18. Chapter Eight: Prediction of mitochondrial targeting signals and their cleavage sites - p. 162 19. Chapter Nine: Monitoring mitochondrial precursor processing and presequence peptide degradation - p. 194 20. Chapter Ten: Analysis of mitochondrial targeting signal cleavage and protein processing by mass spectrometry - p. 216 21. Chapter Eleven: Analysis of targeting signals for mitochondrial intermembrane space import - p. 244 22. Chapter Twelve: Analysis and prediction of internal mitochondrial targeting signals - p. 264 23. Section 4 Mitochondrial precursor proteins in the cytosol - p. 281 24. Chapter Thirteen: Monitoring and analysis of mitochondrial precursor protein aggregates in the cytosol - p. 288 25. Chapter Fourteen: In vitro translation in yeast extract to study interactions with cytosolic chaperones - p. 314 26. Section 5 Mitochondrial protein translocation - p. 343 27. Chapter Fifteen: In vitro import of mitochondrial precursor proteins into yeast mitochondria - p. 348 28. Chapter Sixteen: Monitoring the in vitro import and assembly of mitochondrial precursor proteins into mammalian mitochondria - p. 366 29. Chapter Seventeen: Import of mitochondrial precursor proteins into mitochondria of semi-intact yeast cells - p. 392 30. Chapter Eighteen: The MitoLuc assay for the analysis of the mechanism of mitochondrial protein import - p. 408 31. Chapter Nineteen: Monitoring mitochondrial protein import by live cell imaging - p. 438 32. Chapter Twenty: Quantifying mitochondrial protein import by mePRODmt proteomics - p. 450 33. Chapter Twenty One: Methods to analyze mitochondrial protein translocation in plant mitochondria - p. 476 34. Section 6 Mitochondrial gene expression and translation - p. 497 35. Chapter Twenty Two: In organello silencing of mitochondrial gene expression - p. 502 36. Chapter Twenty Three: Assays to monitor mitochondrial translation - p. 520 37. Chapter Twenty Four: Analysis of mitochondrial translation using click chemistry - p. 534
Mitochondrial Translocases Part A series, highlights new advances in the field, with this new volume presenting interesting chapters. Each chapter is written by an international board of authors.
1. Cover image - p. i 2. Title page - p. ii 3. Table of Contents - p. iii 4. Series Page - p. iii 5. Copyright - p. iii 6. Contributors - p. iii 7. Preface - p. iii 8. Section 1 Isolation of mitochondria - p. p27 9. Chapter One: Isolation of yeast mitochondria by differential centrifugation - p. 4 10. Chapter Two: Isolation of yeast mitochondria by affinity purification using magnetic beads - p. 21 11. Chapter Three: A modified procedure for separating yeast peroxisomes from mitochondria - p. 38 12. Section 2 Analysis of mitochondrial proteins - p. 55 13. Chapter Four: Subcellular fractionation by differential centrifugation for mitochondrial studies - p. 62 14. Chapter Five: Monitoring mitochondrial localization of dual localized proteins using a Bi-Genomic Mitochondrial-Split-GFP - p. 76 15. Chapter Six: Analysis of mitochondrial biogenesis and protein localization by genetic screens and automated imaging - p. 98 16. Chapter Seven: Methods to monitor mitochondrial disulfide bonds - p. 126 17. Section 3 Prediction, analysis and processing of mitochondrial targeting signals - p. 156 18. Chapter Eight: Prediction of mitochondrial targeting signals and their cleavage sites - p. 162 19. Chapter Nine: Monitoring mitochondrial precursor processing and presequence peptide degradation - p. 194 20. Chapter Ten: Analysis of mitochondrial targeting signal cleavage and protein processing by mass spectrometry - p. 216 21. Chapter Eleven: Analysis of targeting signals for mitochondrial intermembrane space import - p. 244 22. Chapter Twelve: Analysis and prediction of internal mitochondrial targeting signals - p. 264 23. Section 4 Mitochondrial precursor proteins in the cytosol - p. 281 24. Chapter Thirteen: Monitoring and analysis of mitochondrial precursor protein aggregates in the cytosol - p. 288 25. Chapter Fourteen: In vitro translation in yeast extract to study interactions with cytosolic chaperones - p. 314 26. Section 5 Mitochondrial protein translocation - p. 343 27. Chapter Fifteen: In vitro import of mitochondrial precursor proteins into yeast mitochondria - p. 348 28. Chapter Sixteen: Monitoring the in vitro import and assembly of mitochondrial precursor proteins into mammalian mitochondria - p. 366 29. Chapter Seventeen: Import of mitochondrial precursor proteins into mitochondria of semi-intact yeast cells - p. 392 30. Chapter Eighteen: The MitoLuc assay for the analysis of the mechanism of mitochondrial protein import - p. 408 31. Chapter Nineteen: Monitoring mitochondrial protein import by live cell imaging - p. 438 32. Chapter Twenty: Quantifying mitochondrial protein import by mePRODmt proteomics - p. 450 33. Chapter Twenty One: Methods to analyze mitochondrial protein translocation in plant mitochondria - p. 476 34. Section 6 Mitochondrial gene expression and translation - p. 497 35. Chapter Twenty Two: In organello silencing of mitochondrial gene expression - p. 502 36. Chapter Twenty Three: Assays to monitor mitochondrial translation - p. 520 37. Chapter Twenty Four: Analysis of mitochondrial translation using click chemistry - p. 534
