Mitochondrial Transplantation and Transfer: Biology, Methods, Applications, and Disease, a volume in the Translational and Applied Bioenergetics series, offers a detailed overview of mitochondrial transplantation across fundamental biology, disease and early therapeutics research. Here, more than twenty experts in the field discuss transplantation and transfer of healthy mitochondria and define future steps to improve new therapeutic applications across a variety of diseases. Emphasis is placed on the physiology of mitochondrial transfer, isolation processes and their applications, transfer methods, and diagnostic and treatment use across cardiovascular, metabolic, and neurological diseases, among other disease types.
Step-by-step instruction is provided around research protocols and methods discussed, along with careful attention to the ethical implications of mitochondrial transplantation research and application.
1. Section I: Introduction - p. xviii 2. Chapter 1. Mitochondrial transplantation, methods and mitochondrial dysfunction related diseases: An overview - p. 3 3. Chapter 2. Mitochondria and cell metabolism - p. 17 4. Chapter 3. Mitochondrial dynamics and mitochondrial biogenesis - p. 35 5. Chapter 4. Mitochondrial DNA and heteroplasmy - p. 59 6. Section II: Physiological mitochondrial transfer - p. 102 7. Chapter 5. Tunneling nanotubes - p. 105 8. Chapter 6. The role of extracellular vesicles and gap junctions in inter-cellular mitochondrial transfer - p. 133 9. Section III: Mitochondrial transplantation from isolation process to application - p. 155 10. Chapter 7. Isolation process for mitochondrial transplantation - p. 159 11. Chapter 8. Determination of mitochondrial number and function - p. 173 12. Chapter 9. Storage of isolated mitochondria - p. 185 13. Section IV: Application methods and limitations of mitochondrial transplantation - p. 196 14. Chapter 10. Mitochondrial transplantation methods - p. 199 15. Chapter 11. Mitochondrial replacement therapy - p. 215 16. Chapter 12. Application route of mitochondrial transplantation - p. 231 17. Chapter 13. Limitations of mitochondrial transplantation - p. 281 18. Chapter 14. The ethical issues of mitochondrial transplantation and transfer - p. 313 19. Section V: Cardiovascular diseases and mitochondrial transplantation - p. 322 20. Chapter 15. Cardiovascular diseases and pathophysiology - p. 327 21. Chapter 16. Cardiovascular diseases and mitochondrial transplantation: Basic and preclinical studies and transitions to clinical applications - p. 343 22. Section VI: Neurological diseases and mitochondrial transplantation - p. 358 23. Chapter 17. Neurological diseases and pathophysiology - p. 361 24. Chapter 18. Preclinical studies - p. 375 25. Section VII: Other diseases and mitochondrial transplantation - p. 384 26. Chapter 19. Hereditary mitochondrial diseases and mitochondrial transplantation - p. 387 27. Chapter 20. Mitochondrial transplantation in kidney injury - p. 411 28. Chapter 21. Cancer and mitochondrial transplantation, and novel insights into its application in the cancer-like phenotype of pulmonary arterial hypertension - p. 435 29. Chapter 22. Diabetes and mitochondrial transplantation - p. 453 30. Chapter 23. Aging and mitochondrial transplantation - p. 475 31. Chapter 24. Mitochondrial transfer in the immune compartment - p. 493 32. Chapter 25. The past, present, and close future of mitochondria as a therapeutic agent - p. 517 33. Index - p. 533
Mitochondrial Transplantation and Transfer: Biology, Methods, Applications, and Disease, a volume in the Translational and Applied Bioenergetics series, offers a detailed overview of mitochondrial transplantation across fundamental biology, disease and early therapeutics research. Here, more than twenty experts in the field discuss transplantation and transfer of healthy mitochondria and define future steps to improve new therapeutic applications across a variety of diseases. Emphasis is placed on the physiology of mitochondrial transfer, isolation processes and their applications, transfer methods, and diagnostic and treatment use across cardiovascular, metabolic, and neurological diseases, among other disease types.
Step-by-step instruction is provided around research protocols and methods discussed, along with careful attention to the ethical implications of mitochondrial transplantation research and application.
1. Section I: Introduction - p. xviii 2. Chapter 1. Mitochondrial transplantation, methods and mitochondrial dysfunction related diseases: An overview - p. 3 3. Chapter 2. Mitochondria and cell metabolism - p. 17 4. Chapter 3. Mitochondrial dynamics and mitochondrial biogenesis - p. 35 5. Chapter 4. Mitochondrial DNA and heteroplasmy - p. 59 6. Section II: Physiological mitochondrial transfer - p. 102 7. Chapter 5. Tunneling nanotubes - p. 105 8. Chapter 6. The role of extracellular vesicles and gap junctions in inter-cellular mitochondrial transfer - p. 133 9. Section III: Mitochondrial transplantation from isolation process to application - p. 155 10. Chapter 7. Isolation process for mitochondrial transplantation - p. 159 11. Chapter 8. Determination of mitochondrial number and function - p. 173 12. Chapter 9. Storage of isolated mitochondria - p. 185 13. Section IV: Application methods and limitations of mitochondrial transplantation - p. 196 14. Chapter 10. Mitochondrial transplantation methods - p. 199 15. Chapter 11. Mitochondrial replacement therapy - p. 215 16. Chapter 12. Application route of mitochondrial transplantation - p. 231 17. Chapter 13. Limitations of mitochondrial transplantation - p. 281 18. Chapter 14. The ethical issues of mitochondrial transplantation and transfer - p. 313 19. Section V: Cardiovascular diseases and mitochondrial transplantation - p. 322 20. Chapter 15. Cardiovascular diseases and pathophysiology - p. 327 21. Chapter 16. Cardiovascular diseases and mitochondrial transplantation: Basic and preclinical studies and transitions to clinical applications - p. 343 22. Section VI: Neurological diseases and mitochondrial transplantation - p. 358 23. Chapter 17. Neurological diseases and pathophysiology - p. 361 24. Chapter 18. Preclinical studies - p. 375 25. Section VII: Other diseases and mitochondrial transplantation - p. 384 26. Chapter 19. Hereditary mitochondrial diseases and mitochondrial transplantation - p. 387 27. Chapter 20. Mitochondrial transplantation in kidney injury - p. 411 28. Chapter 21. Cancer and mitochondrial transplantation, and novel insights into its application in the cancer-like phenotype of pulmonary arterial hypertension - p. 435 29. Chapter 22. Diabetes and mitochondrial transplantation - p. 453 30. Chapter 23. Aging and mitochondrial transplantation - p. 475 31. Chapter 24. Mitochondrial transfer in the immune compartment - p. 493 32. Chapter 25. The past, present, and close future of mitochondria as a therapeutic agent - p. 517 33. Index - p. 533
