Tissue Culture
1. Introduction to Primary Tissue Dissociation - p. xxvii 2. CHAPTER 1: Pronase - p. 1 3. CHAPTER 2: Trypsin: A. Mammalian Tissues - p. 4 4. CHAPTER 2: Trypsin: B. High Yield Method for Kidney Tissue - p. 7 5. CHAPTER 2: Trypsin: C. Marine Teleost Fish Tissues - p. 11 6. CHAPTER 2: Trypsin: D. Amphibian Tissues - p. 13 7. CHAPTER 3: Collagenase Treatment of Chick Heart and Thyroid - p. 15 8. CHAPTER 4: Sequential Enzyme Treatment of Mouse Mammary Gland - p. 20 9. CHAPTER 5: Nonenzymatic Dissociations: A. Leukocyte Cell Separation on Glass - p. 24 10. CHAPTER 5: Nonenzymatic Dissociations: B. Specific Cell Fractionation on Chemically Derivatized Surfaces - p. 28 11. SECTION II: PREPARATION OF PRIMARY CULTURES - p. 36 12. Introduction to Preparation of Primary Cultures - p. 36 13. CHAPTER 1: Human Skin Fibroblasts - p. 37 14. CHAPTER 2: Fetal Human Diploid Cells - p. 42 15. CHAPTER 3: Human Mammary Tumors - p. 44 16. CHAPTER 4: Human Glia Cells - p. 49 17. CHAPTER 5: Human Liver Cells - p. 53 18. CHAPTER 6: Human Blood Leukocytes - p. 57 19. CHAPTER 7: Amniocentesis - p. 61 20. CHAPTER 8: Human Lymph Nodes - p. 64 21. CHAPTER 9: Human Biopsy Material from Genetic Abnormalities - p. 68 22. CHAPTER 10: Mammalian Myocardial Cells - p. 71 23. CHAPTER 11: Mammalian Glomerular Cells - p. 81 24. CHAPTER 12: Organotypic Mammalian Central and Peripheral Nerve Tissue - p. 85 25. CHAPTER 13: Marmoset Bone Marrow - p. 92 26. CHAPTER 14: Monkey Kidney Cells - p. 96 27. CHAPTER 15: Rabbit Kidney and Skin - p. 101 28. CHAPTER 16: Rat Skeletal Muscle Cells - p. 105 29. CHAPTER 17: Rat Pituitary Explants - p. 113 30. CHAPTER 18: Chick Embryo Cells - p. 118 31. CHAPTER 19: Frog Embryos (Haploid Lines) - p. 122 32. CHAPTER 20: Adult Amphibian Tissues and Leukocytes - p. 128 33. CHAPTER 21: Marine Teleost Fish Tissues - p. 132 34. CHAPTER 22: Fish Lymphocytes and Blastogenesis - p. 134 35. CHAPTER 23: Freshwater Fishes - p. 137 36. CHAPTER 24: Goldfish Tissues - p. 142 37. CHAPTER 25: Reptiles - p. 146 38. CHAPTER 26: Dipteran Embryos and Larvae (Diploid Lines) - p. 149 39. CHAPTER 27: Cockroach and Grasshopper Embryo Tissue - p. 152 40. CHAPTER 28: Plants: Haploid Tissue Cultures - p. 156 41. CHAPTER 29: Aspen Callus - p. 160 42. CHAPTER 30: Plant Embryos - p. 165 43. CHAPTER 31: Somatic Plant Cells - p. 169 44. CHAPTER 32: Roots - p. 172 45. CHAPTER 33: Leaves - p. 178 46. SECTION III: CELL HARVESTING - p. 181 47. Introduction to Cell Harvesting - p. 181 48. CHAPTER 1: Pronase - p. 183 49. CHAPTER 2: Trypsinization of Diploid Human Fibroblasts - p. 187 50. CHAPTER 3: Transplantable Rat Tumors and Cold Temperature Release - p. 191 51. CHAPTER 4: Cells at Defined Stages of the Cell Cycle: A. Selection of Mitotic Cells from Monolayer Culture - p. 194 52. CHAPTER 4: Cells at Defined Stages of the Cell Cycle: B. Sedimentation in Sucrose and Ficoll Gradients of Cells Grown in Suspension Culture - p. 197 53. CHAPTER 5: Processing Cells for Enzyme Assays - p. 203 54. SECTION IV: REPLICATE CULTURE METHODS - p. 207 55. Introduction to Replicate Culture Methods - p. 207 56. CHAPTER 1: Monolayer Cultures - p. 209 57. CHAPTER 2: Plant Cell Suspension Cultures - p. 214 58. CHAPTER 3: Subculturing Human Diploid Fibroblast Cultures - p. 219 59. CHAPTER 4: Apparatus for Changing Tissue Culture Media - p. 223 60. CHAPTER 5: Single “Subculture” in Situ Using Glass Beads - p. 225 61. CHAPTER 6: Maintenance of Diploid Fibroblast Cultures as Nonmitotic Populations - p. 230 62. SECTION V: SINGLE CELL ISOLATIONS AND CLONING - p. 233 63. Introduction to Single Cell Isolations and Cloning - p. 233 64. CHAPTER 1: Animal Cells: A. Capillary Techniques - p. 235 65. CHAPTER 1: Animal Cells: B. Microdrop Techniques - p. 240 66. CHAPTER 2: Plant Cells - p. 243 67. CHAPTER 3: Dilution Plating and Nutritional Considerations: A. Animal Cells - p. 253 68. CHAPTER 3: Dilution Plating and Nutritional Considerations: B. Plant Cells - p. 260 69. CHAPTER 4: Dilution Plating on Coverslip Fragments - p. 263 70. CHAPTER 5: Microtest Plates: A. Single Cell Clones - p. 265 71. CHAPTER 5: Microtest Plates: B. Replica Plating - p. 269 72. CHAPTER 6: Low Oxygen Tension Technique - p. 273 73. CHAPTER 7: Soft Agar Techniques - p. 275 74. SECTION VI: PERFUSION AND MASS CULTURE TECHNIQUES - p. 280 75. Introduction to Perfusion and Mass Culture Techniques - p. 280 76. CHAPTER 1: Dual-Rotary Circumfusion System - p. 281 77. CHAPTER 2: Automated Single Rose Chamber for Cardiac and Other Cells - p. 290 78. CHAPTER 3: Culture Dish Perfusion with Cinemicrography - p. 297 79. CHAPTER 4: Sykes-Moore Chamber - p. 302 80. CHAPTER 5: Culture of the Lens (33°) - p. 308 81. CHAPTER 6: Cardiac Cells in a Capillary Tube - p. 315 82. CHAPTER 7: Artificial Capillaries: An Approach to Tissue Growth in Vitro - p. 320 83. CHAPTER 8: Replicate Roller Bottles - p. 326 84. CHAPTER 9: Replicate Tube Chambers for Bones - p. 330 85. CHAPTER 10: Multisurface Stacked Plate Propagators - p. 332 86. CHAPTER 11: Bulk Culture of Cell Monolayers - p. 337 87. CHAPTER 12: Spin Filter Device for Suspension Cultures - p. 344 88. CHAPTER 13: Automation of Continuous Suspension Cultures by Means of a Nephelostat - p. 350 89. CHAPTER 14: A Chemostat for Single Cell Cultures of Higher Plants - p. 358 90. SECTION VII: CELL PROPAGATION ON MISCELLANEOUS CULTURE SUPPORTS - p. 363 91. Introduction to Cell Propagation on Miscellaneous Culture Supports - p. 363 92. CHAPTER 1: Collagen-Coated Cellulose Sponge - p. 365 93. CHAPTER 2: Microcarrier Cultures of Animal Cells - p. 371 94. CHAPTER 3: Polyester Sheeting - p. 377 95. CHAPTER 4: Protein Polymers as a Substratum for the Modulation of Cell Proliferation in Vitro - p. 378 96. CHAPTER 5: Titanium Disks - p. 382 97. CHAPTER 6: Filter Paper Supports for Plant Cultures - p. 386 98. SECTION VIII: EVALUATION OF CULTURE DYNAMICS - p. 392 99. Introduction to Evaluation of Culture Dynamics - p. 392 100. CHAPTER 1: Hemocytometer Counting - p. 393 101. CHAPTER 2: Electronic Enumeration and Sizing of Cells: A. Primary Tissue Cells - p. 397 102. CHAPTER 2: Electronic Enumeration and Sizing of Cells: B. Tissue Culture Cells - p. 399 103. CHAPTER 3: Dye Exclusion Tests for Cell Viability - p. 405 104. CHAPTER 4: Quantitative Methods for Measuring Cell Growth and Death - p. 407 105. CHAPTER 5: Cell Cycle Analysis: A. Mammalian Cells - p. 411 106. CHAPTER 5: Cell Cycle Analysis: B. Control and Analysis of the Mitotic Cycle in Cultured Plant Root Meristems - p. 422 107. SECTION IX: RECENT TECHNIQUES FACILITATING MICROSCOPIC OBSERVATION OF CELLS - p. 428 108. Introduction to Recent Techniques Facilitating Microscopic Observation of Cells - p. 428 109. CHAPTER 1: Contrast Methods in the Microscopy of Living Tissue - p. 429 110. CHAPTER 2: Rapid Embedding of Cell Culture Monolayers and Suspensions - p. 435 111. CHAPTER 3: Embedding in Situ - p. 437 112. CHAPTER 4: Vertical Sectioning: A. Cells on Millipore Filters - p. 442 113. CHAPTER 4: Vertical Sectioning: B. Cells in Plastic Flasks - p. 447 114. CHAPTER 4: Vertical Sectioning: C. Cells Grown on Decalcified Eggshell Membrane with Special Reference to Electron Microscopic Study - p. 451 115. CHAPTER 5: Hydraulically Operated Microscope Stage - p. 457 116. CHAPTER 6: Culture Dish for High Resolution Microscopy - p. 462 117. SECTION X: CELL HYBRIDIZATION - p. 466 118. Introduction to Cell Hybridization - p. 466 119. CHAPTER 1: Somatic Hybridization in Studies of Heredity of Cell Malignancy - p. 467 120. CHAPTER 2: Production and Characterization of Proliferating Somatic Cell Hybrids - p. 474 121. CHAPTER 3: Plant Protoplast Culture - p. 499 122. SECTION XI: VIRUS PROPAGATION AND ASSAY - p. 505 123. Introduction to Virus Propagation and Assay - p. 505 124. CHAPTER 1: Microculture Procedures: A. Vesicular Stomatitis Virus - p. 507 125. CHAPTER 1: Microculture Procedures: B. Simian Virus 40 - p. 516 126. CHAPTER 1: Microculture Procedures: C. Antibody Neutralization Surveys - p. 523 127. CHAPTER 2: Quantal and Enumerative Titration of Virus in Cell Cultures - p. 526 128. CHAPTER 3: Diagnosis of Virus-Infected Cultures with Fluorescein-Labeled Antisera - p. 531 129. CHAPTER 4: Rapid Method for Detection of Budding Virus by Electron Microscopy - p. 534 130. CHAPTER 5: Monitoring for Presence of Oncogenic Virus in Tissue Culture - p. 538 131. CHAPTER 6: Tobacco Mosaic Virus in Plant Tissue Culture - p. 548 132. SECTION XII: PRODUCTION OF HORMONES AND INTERCELLULAR SUBSTANCES - p. 558 133. Introduction to Production of Hormones and Intercellular Substances - p. 558 134. CHAPTER 1: Growth Hormone and Prolactin from Rat Pituitary Tumor Cells - p. 559 135. CHAPTER 2: Enhancement of Growth Hormone Production by Cortisol - p. 569 136. CHAPTER 3: Human Chorionic Gonadotropin - p. 574 137. CHAPTER 4: Parathyroid Hormone and Calcitonin - p. 577 138. CHAPTER 5: Measurement of Collagen Synthesis - p. 585 139. CHAPTER 6: Evaluation of Milk Protein Synthesis - p. 588 140. CHAPTER 7: Interferon Yield Versus Cell Genotype - p. 592 141. CHAPTER 8: Immunoglobulins - p. 598 142. SECTION XIII: DIAGNOSIS AND UNDERSTANDING OF DISEASE - p. 607 143. Introduction to Diagnosis and Understanding of Disease - p. 607 144. CHAPTER 1: Some Cell Culture Procedures in Diagnostic Medical Virology - p. 609 145. CHAPTER 2: Diagnostic Use of Cell Cultures Initiated from Amniocentesis - p. 616 146. CHAPTER 3: Expressions of Citrullinemia in Skin Fibroblast Cultures - p. 622 147. CHAPTER 4: The Hurler Cell in Culture - p. 626 148. CHAPTER 5: Characteristics of Fibroblasts Cultured from Patients with Galactosemia or Galactokinase Deficiency - p. 632 149. CHAPTER 6: Evaluation of Amino Acid Metabolism in Maple Syrup Urine Disease - p. 638 150. CHAPTER 7: Malignant Transformation of Cells in Culture Using Oncogenic Chemicals - p. 643 151. CHAPTER 8: Malignant Transformation of Cells in Culture - p. 652 152. CHAPTER 9: Some in Vitro Models of Normal and Malignant Cell Interaction - p. 658 153. CHAPTER 10: Growth and Isolation of Nutritional Variants - p. 664 154. SECTION XIV: QUALITY CONTROL MEASURES - p. 667 155. Introduction to Quality Control Measures - p. 667 156. CHAPTER 1: Glassware Preparation, Sterilizations, and Use of Laminar Flow Systems - p. 669 157. CHAPTER 2: Purification and Standardization of Water for Tissue Culture - p. 673 158. CHAPTER 3: A Surveillance Procedure Applied to Sera - p. 676 159. CHAPTER 4: Preparation of Sera for Nutritional Studies - p. 681 160. CHAPTER 5: Sample Preparations of Media: A. Vertebrate Cells - p. 685 161. CHAPTER 5: Sample Preparations of Media: B. Invertebrate Cells - p. 691 162. CHAPTER 5: Sample Preparations of Media: C. Plant Cultures - p. 697 163. CHAPTER 6: Determination and Survey of Osmolality in Culture Media - p. 702 164. CHAPTER 7: Control of Culture pH with Synthetic Buffers - p. 708 165. CHAPTER 8: Freezing, Storage, and Recovery of Cell Stocks - p. 711 166. CHAPTER 9: Detection, Elimination, and Prevention of Bacteria and Fungi in Tissue Cultures - p. 717 167. CHAPTER 10: Screening Tissue Cultures for Mycoplasma Infections - p. 721 168. CHAPTER 11: Mycoplasma Contamination of Cell Cultures: Incidence, Source, Prevention, and Problems of Elimination - p. 728 169. CHAPTER 12: Elimination of Pathogens from Shoot Tip Cultures - p. 734 170. CHAPTER 13: Contamination of Tissue Culture and Its Cure: Some Examples - p. 738 171. CHAPTER 14: Serological Identifications of Cells in Culture: A. Animal Cells by Fluorescent-Labeled Antibody - p. 743 172. CHAPTER 14: Serological Identifications of Cells in Culture: B. Poikilotherm Cell Identity by Cytotoxic Antibody Test - p. 749 173. CHAPTER 14: Serological Identifications of Cells in Culture: C. Invertebrate Cell Cultures - p. 752 174. CHAPTER 14: Serological Identifications of Cells in Culture: D. Detection of HL-A Antigens on Cultured Normal Human Diploid Fibroblasts - p. 757 175. CHAPTER 15: Karyology of Cells in Culture: A. Preparation and Analysis of Karyotypes and Idiograms - p. 763 176. CHAPTER 15: Karyology of Cells in Culture: B. Harvesting Human Leukocyte Cultures for Chromosome Cytology - p. 767 177. CHAPTER 15: Karyology of Cells in Culture: C. Constitutive Heterochromatin (C-Bands) - p. 772 178. CHAPTER 15: Karyology of Cells in Culture: D. Fluorescent Banding of Chromosomes (Q-bands) - p. 777 179. CHAPTER 15: Karyology of Cells in Culture: E. Trypsin Technique to Reveal G-Bands - p. 781 180. CHAPTER 15: Karyology of Cells in Culture: F. Characteristics of Insect Cells - p. 787 181. CHAPTER 15: Karyology of Cells in Culture: G. Characteristics of Plant Cells - p. 790 182. CHAPTER 16: Biochemical Identification of Cells in Culture: A. Human Cell Lines by Enzyme Polymorphism - p. 796 183. CHAPTER 16: Biochemical Identification of Cells in Culture: B. Enzymatic “Fingerprinting” - p. 803 184. CHAPTER 17: Introduction to Cytoenzymological Methods and Isozymes - p. 807 185. CHAPTER 18: Transport and Shipping of Cultures, Including International Regulations - p. 821 186. Author Index - p. 827 187. Subject Index - p. 827
Tissue Culture
1. Introduction to Primary Tissue Dissociation - p. xxvii 2. CHAPTER 1: Pronase - p. 1 3. CHAPTER 2: Trypsin: A. Mammalian Tissues - p. 4 4. CHAPTER 2: Trypsin: B. High Yield Method for Kidney Tissue - p. 7 5. CHAPTER 2: Trypsin: C. Marine Teleost Fish Tissues - p. 11 6. CHAPTER 2: Trypsin: D. Amphibian Tissues - p. 13 7. CHAPTER 3: Collagenase Treatment of Chick Heart and Thyroid - p. 15 8. CHAPTER 4: Sequential Enzyme Treatment of Mouse Mammary Gland - p. 20 9. CHAPTER 5: Nonenzymatic Dissociations: A. Leukocyte Cell Separation on Glass - p. 24 10. CHAPTER 5: Nonenzymatic Dissociations: B. Specific Cell Fractionation on Chemically Derivatized Surfaces - p. 28 11. SECTION II: PREPARATION OF PRIMARY CULTURES - p. 36 12. Introduction to Preparation of Primary Cultures - p. 36 13. CHAPTER 1: Human Skin Fibroblasts - p. 37 14. CHAPTER 2: Fetal Human Diploid Cells - p. 42 15. CHAPTER 3: Human Mammary Tumors - p. 44 16. CHAPTER 4: Human Glia Cells - p. 49 17. CHAPTER 5: Human Liver Cells - p. 53 18. CHAPTER 6: Human Blood Leukocytes - p. 57 19. CHAPTER 7: Amniocentesis - p. 61 20. CHAPTER 8: Human Lymph Nodes - p. 64 21. CHAPTER 9: Human Biopsy Material from Genetic Abnormalities - p. 68 22. CHAPTER 10: Mammalian Myocardial Cells - p. 71 23. CHAPTER 11: Mammalian Glomerular Cells - p. 81 24. CHAPTER 12: Organotypic Mammalian Central and Peripheral Nerve Tissue - p. 85 25. CHAPTER 13: Marmoset Bone Marrow - p. 92 26. CHAPTER 14: Monkey Kidney Cells - p. 96 27. CHAPTER 15: Rabbit Kidney and Skin - p. 101 28. CHAPTER 16: Rat Skeletal Muscle Cells - p. 105 29. CHAPTER 17: Rat Pituitary Explants - p. 113 30. CHAPTER 18: Chick Embryo Cells - p. 118 31. CHAPTER 19: Frog Embryos (Haploid Lines) - p. 122 32. CHAPTER 20: Adult Amphibian Tissues and Leukocytes - p. 128 33. CHAPTER 21: Marine Teleost Fish Tissues - p. 132 34. CHAPTER 22: Fish Lymphocytes and Blastogenesis - p. 134 35. CHAPTER 23: Freshwater Fishes - p. 137 36. CHAPTER 24: Goldfish Tissues - p. 142 37. CHAPTER 25: Reptiles - p. 146 38. CHAPTER 26: Dipteran Embryos and Larvae (Diploid Lines) - p. 149 39. CHAPTER 27: Cockroach and Grasshopper Embryo Tissue - p. 152 40. CHAPTER 28: Plants: Haploid Tissue Cultures - p. 156 41. CHAPTER 29: Aspen Callus - p. 160 42. CHAPTER 30: Plant Embryos - p. 165 43. CHAPTER 31: Somatic Plant Cells - p. 169 44. CHAPTER 32: Roots - p. 172 45. CHAPTER 33: Leaves - p. 178 46. SECTION III: CELL HARVESTING - p. 181 47. Introduction to Cell Harvesting - p. 181 48. CHAPTER 1: Pronase - p. 183 49. CHAPTER 2: Trypsinization of Diploid Human Fibroblasts - p. 187 50. CHAPTER 3: Transplantable Rat Tumors and Cold Temperature Release - p. 191 51. CHAPTER 4: Cells at Defined Stages of the Cell Cycle: A. Selection of Mitotic Cells from Monolayer Culture - p. 194 52. CHAPTER 4: Cells at Defined Stages of the Cell Cycle: B. Sedimentation in Sucrose and Ficoll Gradients of Cells Grown in Suspension Culture - p. 197 53. CHAPTER 5: Processing Cells for Enzyme Assays - p. 203 54. SECTION IV: REPLICATE CULTURE METHODS - p. 207 55. Introduction to Replicate Culture Methods - p. 207 56. CHAPTER 1: Monolayer Cultures - p. 209 57. CHAPTER 2: Plant Cell Suspension Cultures - p. 214 58. CHAPTER 3: Subculturing Human Diploid Fibroblast Cultures - p. 219 59. CHAPTER 4: Apparatus for Changing Tissue Culture Media - p. 223 60. CHAPTER 5: Single “Subculture” in Situ Using Glass Beads - p. 225 61. CHAPTER 6: Maintenance of Diploid Fibroblast Cultures as Nonmitotic Populations - p. 230 62. SECTION V: SINGLE CELL ISOLATIONS AND CLONING - p. 233 63. Introduction to Single Cell Isolations and Cloning - p. 233 64. CHAPTER 1: Animal Cells: A. Capillary Techniques - p. 235 65. CHAPTER 1: Animal Cells: B. Microdrop Techniques - p. 240 66. CHAPTER 2: Plant Cells - p. 243 67. CHAPTER 3: Dilution Plating and Nutritional Considerations: A. Animal Cells - p. 253 68. CHAPTER 3: Dilution Plating and Nutritional Considerations: B. Plant Cells - p. 260 69. CHAPTER 4: Dilution Plating on Coverslip Fragments - p. 263 70. CHAPTER 5: Microtest Plates: A. Single Cell Clones - p. 265 71. CHAPTER 5: Microtest Plates: B. Replica Plating - p. 269 72. CHAPTER 6: Low Oxygen Tension Technique - p. 273 73. CHAPTER 7: Soft Agar Techniques - p. 275 74. SECTION VI: PERFUSION AND MASS CULTURE TECHNIQUES - p. 280 75. Introduction to Perfusion and Mass Culture Techniques - p. 280 76. CHAPTER 1: Dual-Rotary Circumfusion System - p. 281 77. CHAPTER 2: Automated Single Rose Chamber for Cardiac and Other Cells - p. 290 78. CHAPTER 3: Culture Dish Perfusion with Cinemicrography - p. 297 79. CHAPTER 4: Sykes-Moore Chamber - p. 302 80. CHAPTER 5: Culture of the Lens (33°) - p. 308 81. CHAPTER 6: Cardiac Cells in a Capillary Tube - p. 315 82. CHAPTER 7: Artificial Capillaries: An Approach to Tissue Growth in Vitro - p. 320 83. CHAPTER 8: Replicate Roller Bottles - p. 326 84. CHAPTER 9: Replicate Tube Chambers for Bones - p. 330 85. CHAPTER 10: Multisurface Stacked Plate Propagators - p. 332 86. CHAPTER 11: Bulk Culture of Cell Monolayers - p. 337 87. CHAPTER 12: Spin Filter Device for Suspension Cultures - p. 344 88. CHAPTER 13: Automation of Continuous Suspension Cultures by Means of a Nephelostat - p. 350 89. CHAPTER 14: A Chemostat for Single Cell Cultures of Higher Plants - p. 358 90. SECTION VII: CELL PROPAGATION ON MISCELLANEOUS CULTURE SUPPORTS - p. 363 91. Introduction to Cell Propagation on Miscellaneous Culture Supports - p. 363 92. CHAPTER 1: Collagen-Coated Cellulose Sponge - p. 365 93. CHAPTER 2: Microcarrier Cultures of Animal Cells - p. 371 94. CHAPTER 3: Polyester Sheeting - p. 377 95. CHAPTER 4: Protein Polymers as a Substratum for the Modulation of Cell Proliferation in Vitro - p. 378 96. CHAPTER 5: Titanium Disks - p. 382 97. CHAPTER 6: Filter Paper Supports for Plant Cultures - p. 386 98. SECTION VIII: EVALUATION OF CULTURE DYNAMICS - p. 392 99. Introduction to Evaluation of Culture Dynamics - p. 392 100. CHAPTER 1: Hemocytometer Counting - p. 393 101. CHAPTER 2: Electronic Enumeration and Sizing of Cells: A. Primary Tissue Cells - p. 397 102. CHAPTER 2: Electronic Enumeration and Sizing of Cells: B. Tissue Culture Cells - p. 399 103. CHAPTER 3: Dye Exclusion Tests for Cell Viability - p. 405 104. CHAPTER 4: Quantitative Methods for Measuring Cell Growth and Death - p. 407 105. CHAPTER 5: Cell Cycle Analysis: A. Mammalian Cells - p. 411 106. CHAPTER 5: Cell Cycle Analysis: B. Control and Analysis of the Mitotic Cycle in Cultured Plant Root Meristems - p. 422 107. SECTION IX: RECENT TECHNIQUES FACILITATING MICROSCOPIC OBSERVATION OF CELLS - p. 428 108. Introduction to Recent Techniques Facilitating Microscopic Observation of Cells - p. 428 109. CHAPTER 1: Contrast Methods in the Microscopy of Living Tissue - p. 429 110. CHAPTER 2: Rapid Embedding of Cell Culture Monolayers and Suspensions - p. 435 111. CHAPTER 3: Embedding in Situ - p. 437 112. CHAPTER 4: Vertical Sectioning: A. Cells on Millipore Filters - p. 442 113. CHAPTER 4: Vertical Sectioning: B. Cells in Plastic Flasks - p. 447 114. CHAPTER 4: Vertical Sectioning: C. Cells Grown on Decalcified Eggshell Membrane with Special Reference to Electron Microscopic Study - p. 451 115. CHAPTER 5: Hydraulically Operated Microscope Stage - p. 457 116. CHAPTER 6: Culture Dish for High Resolution Microscopy - p. 462 117. SECTION X: CELL HYBRIDIZATION - p. 466 118. Introduction to Cell Hybridization - p. 466 119. CHAPTER 1: Somatic Hybridization in Studies of Heredity of Cell Malignancy - p. 467 120. CHAPTER 2: Production and Characterization of Proliferating Somatic Cell Hybrids - p. 474 121. CHAPTER 3: Plant Protoplast Culture - p. 499 122. SECTION XI: VIRUS PROPAGATION AND ASSAY - p. 505 123. Introduction to Virus Propagation and Assay - p. 505 124. CHAPTER 1: Microculture Procedures: A. Vesicular Stomatitis Virus - p. 507 125. CHAPTER 1: Microculture Procedures: B. Simian Virus 40 - p. 516 126. CHAPTER 1: Microculture Procedures: C. Antibody Neutralization Surveys - p. 523 127. CHAPTER 2: Quantal and Enumerative Titration of Virus in Cell Cultures - p. 526 128. CHAPTER 3: Diagnosis of Virus-Infected Cultures with Fluorescein-Labeled Antisera - p. 531 129. CHAPTER 4: Rapid Method for Detection of Budding Virus by Electron Microscopy - p. 534 130. CHAPTER 5: Monitoring for Presence of Oncogenic Virus in Tissue Culture - p. 538 131. CHAPTER 6: Tobacco Mosaic Virus in Plant Tissue Culture - p. 548 132. SECTION XII: PRODUCTION OF HORMONES AND INTERCELLULAR SUBSTANCES - p. 558 133. Introduction to Production of Hormones and Intercellular Substances - p. 558 134. CHAPTER 1: Growth Hormone and Prolactin from Rat Pituitary Tumor Cells - p. 559 135. CHAPTER 2: Enhancement of Growth Hormone Production by Cortisol - p. 569 136. CHAPTER 3: Human Chorionic Gonadotropin - p. 574 137. CHAPTER 4: Parathyroid Hormone and Calcitonin - p. 577 138. CHAPTER 5: Measurement of Collagen Synthesis - p. 585 139. CHAPTER 6: Evaluation of Milk Protein Synthesis - p. 588 140. CHAPTER 7: Interferon Yield Versus Cell Genotype - p. 592 141. CHAPTER 8: Immunoglobulins - p. 598 142. SECTION XIII: DIAGNOSIS AND UNDERSTANDING OF DISEASE - p. 607 143. Introduction to Diagnosis and Understanding of Disease - p. 607 144. CHAPTER 1: Some Cell Culture Procedures in Diagnostic Medical Virology - p. 609 145. CHAPTER 2: Diagnostic Use of Cell Cultures Initiated from Amniocentesis - p. 616 146. CHAPTER 3: Expressions of Citrullinemia in Skin Fibroblast Cultures - p. 622 147. CHAPTER 4: The Hurler Cell in Culture - p. 626 148. CHAPTER 5: Characteristics of Fibroblasts Cultured from Patients with Galactosemia or Galactokinase Deficiency - p. 632 149. CHAPTER 6: Evaluation of Amino Acid Metabolism in Maple Syrup Urine Disease - p. 638 150. CHAPTER 7: Malignant Transformation of Cells in Culture Using Oncogenic Chemicals - p. 643 151. CHAPTER 8: Malignant Transformation of Cells in Culture - p. 652 152. CHAPTER 9: Some in Vitro Models of Normal and Malignant Cell Interaction - p. 658 153. CHAPTER 10: Growth and Isolation of Nutritional Variants - p. 664 154. SECTION XIV: QUALITY CONTROL MEASURES - p. 667 155. Introduction to Quality Control Measures - p. 667 156. CHAPTER 1: Glassware Preparation, Sterilizations, and Use of Laminar Flow Systems - p. 669 157. CHAPTER 2: Purification and Standardization of Water for Tissue Culture - p. 673 158. CHAPTER 3: A Surveillance Procedure Applied to Sera - p. 676 159. CHAPTER 4: Preparation of Sera for Nutritional Studies - p. 681 160. CHAPTER 5: Sample Preparations of Media: A. Vertebrate Cells - p. 685 161. CHAPTER 5: Sample Preparations of Media: B. Invertebrate Cells - p. 691 162. CHAPTER 5: Sample Preparations of Media: C. Plant Cultures - p. 697 163. CHAPTER 6: Determination and Survey of Osmolality in Culture Media - p. 702 164. CHAPTER 7: Control of Culture pH with Synthetic Buffers - p. 708 165. CHAPTER 8: Freezing, Storage, and Recovery of Cell Stocks - p. 711 166. CHAPTER 9: Detection, Elimination, and Prevention of Bacteria and Fungi in Tissue Cultures - p. 717 167. CHAPTER 10: Screening Tissue Cultures for Mycoplasma Infections - p. 721 168. CHAPTER 11: Mycoplasma Contamination of Cell Cultures: Incidence, Source, Prevention, and Problems of Elimination - p. 728 169. CHAPTER 12: Elimination of Pathogens from Shoot Tip Cultures - p. 734 170. CHAPTER 13: Contamination of Tissue Culture and Its Cure: Some Examples - p. 738 171. CHAPTER 14: Serological Identifications of Cells in Culture: A. Animal Cells by Fluorescent-Labeled Antibody - p. 743 172. CHAPTER 14: Serological Identifications of Cells in Culture: B. Poikilotherm Cell Identity by Cytotoxic Antibody Test - p. 749 173. CHAPTER 14: Serological Identifications of Cells in Culture: C. Invertebrate Cell Cultures - p. 752 174. CHAPTER 14: Serological Identifications of Cells in Culture: D. Detection of HL-A Antigens on Cultured Normal Human Diploid Fibroblasts - p. 757 175. CHAPTER 15: Karyology of Cells in Culture: A. Preparation and Analysis of Karyotypes and Idiograms - p. 763 176. CHAPTER 15: Karyology of Cells in Culture: B. Harvesting Human Leukocyte Cultures for Chromosome Cytology - p. 767 177. CHAPTER 15: Karyology of Cells in Culture: C. Constitutive Heterochromatin (C-Bands) - p. 772 178. CHAPTER 15: Karyology of Cells in Culture: D. Fluorescent Banding of Chromosomes (Q-bands) - p. 777 179. CHAPTER 15: Karyology of Cells in Culture: E. Trypsin Technique to Reveal G-Bands - p. 781 180. CHAPTER 15: Karyology of Cells in Culture: F. Characteristics of Insect Cells - p. 787 181. CHAPTER 15: Karyology of Cells in Culture: G. Characteristics of Plant Cells - p. 790 182. CHAPTER 16: Biochemical Identification of Cells in Culture: A. Human Cell Lines by Enzyme Polymorphism - p. 796 183. CHAPTER 16: Biochemical Identification of Cells in Culture: B. Enzymatic “Fingerprinting” - p. 803 184. CHAPTER 17: Introduction to Cytoenzymological Methods and Isozymes - p. 807 185. CHAPTER 18: Transport and Shipping of Cultures, Including International Regulations - p. 821 186. Author Index - p. 827 187. Subject Index - p. 827
