Tumor Immunology and Immunotherapy - Integrated Methods Part B, Volume 636 in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Chapters in this update include Quantification methods of Transforming Growth Factor beta (TGF˗β) activity in the setting of cancer immunotherapy, Decoding cancer cell death-driven immune cell recruitment: An in vivo method for site-of-vaccination analyses, Tracking and interrogating tissue-resident and recruited microglia in brain tumors, Metabolomics and lipidomics of the tumor microenvironment, Monitoring abscopal responses to radiation in mice, and much more.
1. Cover image - p. page_i 2. Title page - p. page_i 3. Table of Contents - p. page_i 4. Copyright - p. page_iv 5. Contributors - p. page_xi 6. Preface: More than two decades of modern tumor immunology - p. page_xix 7. Chapter One: A comprehensive approach to gene expression profiling in immune cells - p. page_xxxvi 8. Chapter Two: DIY: Visualizing the immune landscape of tumors using transcriptome and methylome data - p. page_47 9. Chapter Three: Using epigenetic data to estimate immune composition in admixed samples - p. page_76 10. Chapter Four: Metabolomic approaches to study the tumor microenvironment - p. page_92 11. Chapter Five: Immunoscore assay for the immune classification of solid tumors: Technical aspects, improvements and clinical perspectives - p. page_108 12. Chapter Six: Transforming growth factor beta (TGF-β) activity in immuno-oncology studies - p. page_128 13. Chapter Seven: Isolation of DNA from exosomes - p. page_172 14. Chapter Eight: Decoding cancer cell death-driven immune cell recruitment: An in vivo method for site-of-vaccination analyses - p. page_183 15. Chapter Nine: Computational methods in tumor immunology - p. page_207 16. Chapter Ten: Deconvoluting tumor-infiltrating immune cells from RNA-seq data using quanTIseq - p. page_259 17. Chapter Eleven: Concurrent in situ analysis of point mutations and immune infiltrate in FFPE cancers - p. page_285 18. Chapter Twelve: RNA interference screening methods to identify proliferation determinants and mechanisms of resistance to immune attack - p. page_297 19. Chapter Thirteen: An altered miTRAP method for miRNA affinity purification with its pros and cons - p. page_322 20. Chapter Fourteen: Identification of immunomodulatory RNA-binding proteins in tumors - p. page_337 21. Chapter Fifteen: Techniques for the generation of humanized mouse models for immuno-oncology - p. page_350
Tumor Immunology and Immunotherapy - Integrated Methods Part B, Volume 636 in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Chapters in this update include Quantification methods of Transforming Growth Factor beta (TGF˗β) activity in the setting of cancer immunotherapy, Decoding cancer cell death-driven immune cell recruitment: An in vivo method for site-of-vaccination analyses, Tracking and interrogating tissue-resident and recruited microglia in brain tumors, Metabolomics and lipidomics of the tumor microenvironment, Monitoring abscopal responses to radiation in mice, and much more.
1. Cover image - p. page_i 2. Title page - p. page_i 3. Table of Contents - p. page_i 4. Copyright - p. page_iv 5. Contributors - p. page_xi 6. Preface: More than two decades of modern tumor immunology - p. page_xix 7. Chapter One: A comprehensive approach to gene expression profiling in immune cells - p. page_xxxvi 8. Chapter Two: DIY: Visualizing the immune landscape of tumors using transcriptome and methylome data - p. page_47 9. Chapter Three: Using epigenetic data to estimate immune composition in admixed samples - p. page_76 10. Chapter Four: Metabolomic approaches to study the tumor microenvironment - p. page_92 11. Chapter Five: Immunoscore assay for the immune classification of solid tumors: Technical aspects, improvements and clinical perspectives - p. page_108 12. Chapter Six: Transforming growth factor beta (TGF-β) activity in immuno-oncology studies - p. page_128 13. Chapter Seven: Isolation of DNA from exosomes - p. page_172 14. Chapter Eight: Decoding cancer cell death-driven immune cell recruitment: An in vivo method for site-of-vaccination analyses - p. page_183 15. Chapter Nine: Computational methods in tumor immunology - p. page_207 16. Chapter Ten: Deconvoluting tumor-infiltrating immune cells from RNA-seq data using quanTIseq - p. page_259 17. Chapter Eleven: Concurrent in situ analysis of point mutations and immune infiltrate in FFPE cancers - p. page_285 18. Chapter Twelve: RNA interference screening methods to identify proliferation determinants and mechanisms of resistance to immune attack - p. page_297 19. Chapter Thirteen: An altered miTRAP method for miRNA affinity purification with its pros and cons - p. page_322 20. Chapter Fourteen: Identification of immunomodulatory RNA-binding proteins in tumors - p. page_337 21. Chapter Fifteen: Techniques for the generation of humanized mouse models for immuno-oncology - p. page_350
