The various cell types have traditionally been recognized and classified according to their appearance in the light microscope following the process of fixing, processing, sectioning, and staining tissues that is known as histology. Classical histology has been augmented by
immunohistochemistry (the use of specific antibodies to stain particular molecular species in situ). Immunohistochemistry has allowed the identification of many more cell types than could be visualized by classical histology, particularly in the immune system and among the scattered hormone-secreting cells of the endocrine system.
This book discusses all aspects of immunohistochemistry and in situ hybridization technologies and the important role they play in reaching a cancer diagnosis. It provides step-by-step instructions on the methods of additional molecular technologies such as DNA microarrays, and microdissection, along with the benefits and limitations of each method. The topics of region-specific gene expression, its role in cancer development and the techniques that assist in the understanding of the molecular basis of disease are relevant and necessary in science today, ensuring a wide audience for this book.
* The only book available that translates molecular genetics into cancer diagnosis
* Provides the readers with tools necessary to perform and optimize sensitive, powerful techniques, including immunohistochemistry and fluorescence in situ hybridization, used in tumor diagnosis
* Written by experts in this field, the book provides theoretical considerations as well as practical approaches to carry out effectively these techniques
* Offers suggestions, tips, cautions, and guidelines to avoid artifacts and misdiagnosis
* Introduces new techniques to detect genes and proteins involved in the initiation and progression of cancer
* Covers the latest developments and a wide range of applications to the detection of antigens and single-copy DNA and RNA
* Written in a uniform format, each chapter includes Introduction, Materials required, step-by-step detailed Methods, Results, Discussion, and comprehensive up-to-date References
1. Introduction to Volumes 2 and 3 2. Prologue 3. Selected Definitions 4. Classification Scheme of Human Cancers 5. Lung and Breast Carcinomas 6. Comparison of Immunohistochemistry, in situ Hybridization, Fluorescence in situ Hybridization, and Chromogenic in situ Hybridization 7. Comparison of Chromogenic in situ Hybridization, Fluorescence in situ Hybridization, and Immunohistochemistry 8. Target and Signal Amplification to Increase the Sensitivity of in situ Hybridization 9. Polymerase Chain Reaction Technology 10. DNA Microarrays Technology 11. Tissue Microarrays and Their Modifications in High-Throughput Analysis of Clinical Specimens 12. Gene Expression Profiling Using Microdissection in Cancer Tissues 13. Differential Display of Gene Expression in Human Carcinomas 14. Serial Analysis of Gene Expression in Human Diseases 15. Lung Carcinoma: An Introduction 16. Histopathological Classification Phenotype and Molecular Pathology of Lung Tumors 17. Immunohistochemistry and in situ Hybridization of Mucin in Lung Carcinoma 18. Immunohistochemical Expression of MDM2 in Lung Carcinoma 19. Immunohistochemical Expression of E2F1 and p14ARF in Lung Carcinoma 20. Role of Immunohistochemical Expression of Beta-Catenin in Lung Carcinoma 21. Immunohistochemistry of Laminin-5 in Lung Carcinoma 22. Role of Immunohistochemical Expression of Caveolin-1 in Lung Carcinoma 23. Role of Thyroid Transcription Factor-1 in Pulmonary Adenocarcinoma 24. Role of Global Methylation of DNA in Lung Carcinoma 25. Immunohistochemical and Molecular Pathology of Angiogenesis in Primary Lung Adenocarcinoma 26. Immunohistochemistry of Human Leukocyte Antigen Expression in Lung Carcinoma 27. Immunohistochemistry and in situ Hybridization of Telomerase Expression in Lung Carcinoma 28. Use of Fluorescence in situ Hybridization in Detecting Lung Cancer Cells 29. Immunohistochemistry of BCL-2 Gene Expression in Lung Carcinoma 30. Breast Carcinoma: An Introduction 31. Expression of Vascular Endothelial Growth Factor Receptor-2/Flk-1/ KDR in Breast Carcinoma 32. HER-2/neu Gene Amplification and Protein Overexpression in Breast Carcinoma: Immunohistochemistry and Fluorescence in situ Hybridization 33. HER-2/neu Amplification Detected by Fluorescence in situ Hybridization in Cytological Samples from Breast Cancer 34. Detection of HER-2 Oncogene in Human Breast Carcinoma Using Chromogenic in situ Hybridization 35. Immunohistochemical Evaluation of Sentinel Lymph Nodes in Breast Carcinoma 36. CD10 Expression in Normal Breast and Breast Cancer Tissues 37. Role of Immunohistochemical Expression of AKT Protein in Breast Carcinoma 38. Expression of Extracellular Matrix Proteins in Breast Cancer 39. Immunohistochemistry of Adhesion Molecule CEACAM1 Expression in Breast Carcinoma 40. Role of Cadherins in Breast Cancer 41. Immunohistochemical Expression of Erythropoietin and Erythropoietin Receptor in Breast Carcinoma 42. Loss of BRCA1Gene Expression in Breast Carcinoma 43. Role of Immunohistochemical Detection of BRCA1 in Breast Cancer 44. Fluorescence in situ Hybridization of BRCA1Gene in Breast Carcinoma 45. Immunohistochemistry of c-myc Expression in Breast Carcinoma 46. Immunohistochemical Localization of Neuropilin-1 in Human Breast Carcinoma 47. Role of Epidermal Growth Factor Receptor in Breast Carcinoma 48. Alterations of the Cell Cycle Regulating Proteins in Invasive Breast Cancer 49. Immunohistochemistry of Estrogen Receptor Expression in Breast Carcinoma 50. Immunofluorescence and Immunohistochemical Localization of Progesterone Receptors in Breast Carcinoma 51. Immunohistochemical Expression of Cytosolic Thymidine Kinase in Patients with Breast Carcinoma 52. Immunohistochemical Detection of Melanoma Antigen E (MAGE) Expression in Breast Carcinoma 53. Male Breast Carcinoma: Role of Immunohistochemical Expression of Receptors in Male Breast Carcinoma 54. Detection of Glycoconjugates in Breast Cancer Cell Lines: Confocal Fluorescence Microscopy 55. Expression of the ETV6-NTRK3 Gene Fusion in Human Secretory Breast Carcinoma 56. The Role of CA6 Protein Expression in Breast Carcinoma 57. Immunocytochemistry of Effusions 58. Immunohistochemistry of Needle Cytopunctures of Breast Carcinomas 59. Index
The various cell types have traditionally been recognized and classified according to their appearance in the light microscope following the process of fixing, processing, sectioning, and staining tissues that is known as histology. Classical histology has been augmented by
immunohistochemistry (the use of specific antibodies to stain particular molecular species in situ). Immunohistochemistry has allowed the identification of many more cell types than could be visualized by classical histology, particularly in the immune system and among the scattered hormone-secreting cells of the endocrine system.
This book discusses all aspects of immunohistochemistry and in situ hybridization technologies and the important role they play in reaching a cancer diagnosis. It provides step-by-step instructions on the methods of additional molecular technologies such as DNA microarrays, and microdissection, along with the benefits and limitations of each method. The topics of region-specific gene expression, its role in cancer development and the techniques that assist in the understanding of the molecular basis of disease are relevant and necessary in science today, ensuring a wide audience for this book.
* The only book available that translates molecular genetics into cancer diagnosis
* Provides the readers with tools necessary to perform and optimize sensitive, powerful techniques, including immunohistochemistry and fluorescence in situ hybridization, used in tumor diagnosis
* Written by experts in this field, the book provides theoretical considerations as well as practical approaches to carry out effectively these techniques
* Offers suggestions, tips, cautions, and guidelines to avoid artifacts and misdiagnosis
* Introduces new techniques to detect genes and proteins involved in the initiation and progression of cancer
* Covers the latest developments and a wide range of applications to the detection of antigens and single-copy DNA and RNA
* Written in a uniform format, each chapter includes Introduction, Materials required, step-by-step detailed Methods, Results, Discussion, and comprehensive up-to-date References
1. Introduction to Volumes 2 and 3 2. Prologue 3. Selected Definitions 4. Classification Scheme of Human Cancers 5. Lung and Breast Carcinomas 6. Comparison of Immunohistochemistry, in situ Hybridization, Fluorescence in situ Hybridization, and Chromogenic in situ Hybridization 7. Comparison of Chromogenic in situ Hybridization, Fluorescence in situ Hybridization, and Immunohistochemistry 8. Target and Signal Amplification to Increase the Sensitivity of in situ Hybridization 9. Polymerase Chain Reaction Technology 10. DNA Microarrays Technology 11. Tissue Microarrays and Their Modifications in High-Throughput Analysis of Clinical Specimens 12. Gene Expression Profiling Using Microdissection in Cancer Tissues 13. Differential Display of Gene Expression in Human Carcinomas 14. Serial Analysis of Gene Expression in Human Diseases 15. Lung Carcinoma: An Introduction 16. Histopathological Classification Phenotype and Molecular Pathology of Lung Tumors 17. Immunohistochemistry and in situ Hybridization of Mucin in Lung Carcinoma 18. Immunohistochemical Expression of MDM2 in Lung Carcinoma 19. Immunohistochemical Expression of E2F1 and p14ARF in Lung Carcinoma 20. Role of Immunohistochemical Expression of Beta-Catenin in Lung Carcinoma 21. Immunohistochemistry of Laminin-5 in Lung Carcinoma 22. Role of Immunohistochemical Expression of Caveolin-1 in Lung Carcinoma 23. Role of Thyroid Transcription Factor-1 in Pulmonary Adenocarcinoma 24. Role of Global Methylation of DNA in Lung Carcinoma 25. Immunohistochemical and Molecular Pathology of Angiogenesis in Primary Lung Adenocarcinoma 26. Immunohistochemistry of Human Leukocyte Antigen Expression in Lung Carcinoma 27. Immunohistochemistry and in situ Hybridization of Telomerase Expression in Lung Carcinoma 28. Use of Fluorescence in situ Hybridization in Detecting Lung Cancer Cells 29. Immunohistochemistry of BCL-2 Gene Expression in Lung Carcinoma 30. Breast Carcinoma: An Introduction 31. Expression of Vascular Endothelial Growth Factor Receptor-2/Flk-1/ KDR in Breast Carcinoma 32. HER-2/neu Gene Amplification and Protein Overexpression in Breast Carcinoma: Immunohistochemistry and Fluorescence in situ Hybridization 33. HER-2/neu Amplification Detected by Fluorescence in situ Hybridization in Cytological Samples from Breast Cancer 34. Detection of HER-2 Oncogene in Human Breast Carcinoma Using Chromogenic in situ Hybridization 35. Immunohistochemical Evaluation of Sentinel Lymph Nodes in Breast Carcinoma 36. CD10 Expression in Normal Breast and Breast Cancer Tissues 37. Role of Immunohistochemical Expression of AKT Protein in Breast Carcinoma 38. Expression of Extracellular Matrix Proteins in Breast Cancer 39. Immunohistochemistry of Adhesion Molecule CEACAM1 Expression in Breast Carcinoma 40. Role of Cadherins in Breast Cancer 41. Immunohistochemical Expression of Erythropoietin and Erythropoietin Receptor in Breast Carcinoma 42. Loss of BRCA1Gene Expression in Breast Carcinoma 43. Role of Immunohistochemical Detection of BRCA1 in Breast Cancer 44. Fluorescence in situ Hybridization of BRCA1Gene in Breast Carcinoma 45. Immunohistochemistry of c-myc Expression in Breast Carcinoma 46. Immunohistochemical Localization of Neuropilin-1 in Human Breast Carcinoma 47. Role of Epidermal Growth Factor Receptor in Breast Carcinoma 48. Alterations of the Cell Cycle Regulating Proteins in Invasive Breast Cancer 49. Immunohistochemistry of Estrogen Receptor Expression in Breast Carcinoma 50. Immunofluorescence and Immunohistochemical Localization of Progesterone Receptors in Breast Carcinoma 51. Immunohistochemical Expression of Cytosolic Thymidine Kinase in Patients with Breast Carcinoma 52. Immunohistochemical Detection of Melanoma Antigen E (MAGE) Expression in Breast Carcinoma 53. Male Breast Carcinoma: Role of Immunohistochemical Expression of Receptors in Male Breast Carcinoma 54. Detection of Glycoconjugates in Breast Cancer Cell Lines: Confocal Fluorescence Microscopy 55. Expression of the ETV6-NTRK3 Gene Fusion in Human Secretory Breast Carcinoma 56. The Role of CA6 Protein Expression in Breast Carcinoma 57. Immunocytochemistry of Effusions 58. Immunohistochemistry of Needle Cytopunctures of Breast Carcinomas 59. Index
