Optogenetics is a fast-growing field concerning the invention, and use, of molecules that are genetically expressed in cells, and then either report on cellular physiology in optical form, or enable control of specific pathways in cells when actuated by light. This book reviews the current state, as well as the underlying principles and future directions, of a diversity of optogenetic tools of importance for observing and controlling cellular signaling and physiology.
These tools are already revolutionizing neuroscience, and are starting to have impact on fields ranging from metabolism to development to cardiology. The book contains a dozen chapters written by world experts on these topics, half focusing on the optogenetic molecular tools themselves, and half on the genetic and hardware approaches that make them powerfully useful in intact tissues and organisms.
1. Cover 2. Table of Contents 3. Copyright Page 4. List of Contributors 5. Preface 6. Chapter 1: A comprehensive concept of optogenetics 7. Chapter 2: Optogenetic excitation of neurons with channelrhodopsins 8. Chapter 3: Genetically encoded molecular tools for light-driven silencing of targeted neurons 9. Chapter 4: Genetically encoded probes for optical imaging of brain electrical activity 10. Chapter 5: Neural activity imaging with genetically encoded calcium indicators 11. Chapter 6: Manipulating cellular processes using optical control of protein–protein interactions 12. Chapter 7: Two-photon optogenetics 13. Chapter 8: Zebrafish as an appealing model for optogenetic studies 14. Chapter 9: Genetic targeting of specific neuronal cell types in the cerebral cortex 15. Chapter 10: Mouse transgenic approaches in optogenetics 16. Chapter 11: Optogenetics in the nonhuman primate 17. Chapter 12: Optogenetic reporters 18. Subject Index 19. Contents of previous volume
Optogenetics is a fast-growing field concerning the invention, and use, of molecules that are genetically expressed in cells, and then either report on cellular physiology in optical form, or enable control of specific pathways in cells when actuated by light. This book reviews the current state, as well as the underlying principles and future directions, of a diversity of optogenetic tools of importance for observing and controlling cellular signaling and physiology.
These tools are already revolutionizing neuroscience, and are starting to have impact on fields ranging from metabolism to development to cardiology. The book contains a dozen chapters written by world experts on these topics, half focusing on the optogenetic molecular tools themselves, and half on the genetic and hardware approaches that make them powerfully useful in intact tissues and organisms.
1. Cover 2. Table of Contents 3. Copyright Page 4. List of Contributors 5. Preface 6. Chapter 1: A comprehensive concept of optogenetics 7. Chapter 2: Optogenetic excitation of neurons with channelrhodopsins 8. Chapter 3: Genetically encoded molecular tools for light-driven silencing of targeted neurons 9. Chapter 4: Genetically encoded probes for optical imaging of brain electrical activity 10. Chapter 5: Neural activity imaging with genetically encoded calcium indicators 11. Chapter 6: Manipulating cellular processes using optical control of protein–protein interactions 12. Chapter 7: Two-photon optogenetics 13. Chapter 8: Zebrafish as an appealing model for optogenetic studies 14. Chapter 9: Genetic targeting of specific neuronal cell types in the cerebral cortex 15. Chapter 10: Mouse transgenic approaches in optogenetics 16. Chapter 11: Optogenetics in the nonhuman primate 17. Chapter 12: Optogenetic reporters 18. Subject Index 19. Contents of previous volume
