Plant Tissue Culture, Third Edition builds on the classroom tested, audience proven manual that has guided users through successful plant culturing A.tumefaciens mediated transformation, infusion technology, the latest information on media components and preparation, and regeneration and morphogenesis along with new exercises and diagrams provide current information and examples.
The included experiments demonstrate major concepts and can be conducted with a variety of plant material that are readily available throughout the year. This book provides a diverse learning experience and is appropriate for both university students and plant scientists.
1. Cover image 2. Title page 3. Table of Contents 4. Copyright 5. Preface 6. Acknowledgments 7. Scheduling and Interrelationships of Exercises 8. Chapter 1. History of Plant Cell Culture 9. Chapter 2. Setup of a Tissue Culture Laboratory 10. Chapter 3. Media Components and Preparation 11. Chapter 4. Explant Preparation 12. Chapter 5. Contamination 13. Chapter 6. Callus Induction 14. Chapter 7. Regeneration and Morphogenesis 15. Chapter 8. Woody Shrubs and Trees 16. Chapter 9. Haploid Plants from Anther Culture 17. Chapter 10. Embryo Rescue 18. Chapter 11. Meristem Culture for Virus-Free Plants 19. Chapter 12. In Vitro Propagation for Commercial Production of Ornamentals 20. Chapter 13. Protoplast Isolation and Fusion 21. Chapter 14. Agrobacterium-Mediated Transformation of Plants 22. Appendix I: Useful Measurements 23. Appendix II: Review of Solution Preparation 24. Appendix III: List of Suppliers 25. Appendix IV: Common Plant Tissue Culture Terms 26. Index
Plant Tissue Culture, Third Edition builds on the classroom tested, audience proven manual that has guided users through successful plant culturing A.tumefaciens mediated transformation, infusion technology, the latest information on media components and preparation, and regeneration and morphogenesis along with new exercises and diagrams provide current information and examples.
The included experiments demonstrate major concepts and can be conducted with a variety of plant material that are readily available throughout the year. This book provides a diverse learning experience and is appropriate for both university students and plant scientists.
1. Cover image 2. Title page 3. Table of Contents 4. Copyright 5. Preface 6. Acknowledgments 7. Scheduling and Interrelationships of Exercises 8. Chapter 1. History of Plant Cell Culture 9. Chapter 2. Setup of a Tissue Culture Laboratory 10. Chapter 3. Media Components and Preparation 11. Chapter 4. Explant Preparation 12. Chapter 5. Contamination 13. Chapter 6. Callus Induction 14. Chapter 7. Regeneration and Morphogenesis 15. Chapter 8. Woody Shrubs and Trees 16. Chapter 9. Haploid Plants from Anther Culture 17. Chapter 10. Embryo Rescue 18. Chapter 11. Meristem Culture for Virus-Free Plants 19. Chapter 12. In Vitro Propagation for Commercial Production of Ornamentals 20. Chapter 13. Protoplast Isolation and Fusion 21. Chapter 14. Agrobacterium-Mediated Transformation of Plants 22. Appendix I: Useful Measurements 23. Appendix II: Review of Solution Preparation 24. Appendix III: List of Suppliers 25. Appendix IV: Common Plant Tissue Culture Terms 26. Index
