Chemical Glycobiology, Volume 597, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume, the first on chemical glycobiology, contains comprehensive chapters on the Discovery of New Glycosidases from Metagenomic Libraries, Structure-guided directed evolution of glycosidases: A case study in engineering a blood group antigen-cleaving enzyme, A Pipeline for Studying and Engineering Single-Subunit Oligosaccharyltransferases, Directed evolution of glycopeptides using mRNA display, Chemoenzymatic Synthesis and Applications of Prokaryote-Specific UDP-Sugars, and Biosynthesis of Legionaminic Acid and its Incorporation into Glycoconjugates.
Readers will find the latest information on this developing area of research, as reported by leaders in the field.
1. Cover image - p. iv 2. Title page - p. iv 3. Table of Contents - p. iv 4. Copyright - p. iv 5. Contributors - p. xi 6. Preface - p. xv 7. Section I: Evolution and Engineering of Glycans and Glycan Processing Enzymes - p. xix 8. Chapter One: Discovery of New Glycosidases From Metagenomic Libraries - p. xix 9. Chapter Two: Structure-Guided Directed Evolution of Glycosidases: A Case Study in Engineering a Blood Group Antigen-Cleaving Enzyme - p. 23 10. Chapter Three: A Pipeline for Studying and Engineering Single-Subunit Oligosaccharyltransferases - p. 53 11. Chapter Four: Directed Evolution of Glycopeptides Using mRNA Display - p. 81 12. Section II: Chemical and Enzymatic Synthesis and Analysis of Glycans and Glycoconjugates - p. 141 13. Chapter Five: Chemoenzymatic Synthesis and Applications of Prokaryote-Specific UDP-Sugars - p. 141 14. Chapter Six: Biosynthesis of Legionaminic Acid and Its Incorporation Into Glycoconjugates - p. 186 15. Chapter Seven: Profiling of Sugar Nucleotides - p. 207 16. Chapter Eight: Syntheses of Fluorescent Gangliosides for the Studies of Raft Domains - p. 238 17. Chapter Nine: Chemoenzymatic Glycan Remodeling of Natural and Recombinant Glycoproteins - p. 263 18. Section III: Glycan-Based Vaccines - p. 281 19. Chapter Ten: Glycoengineered Outer Membrane Vesicles as a Platform for Vaccine Development - p. 281 20. Chapter Eleven: Identification and Design of Synthetic B Cell Epitopes for Carbohydrate-Based Vaccines - p. 310 21. Chapter Twelve: Strategies in the Design and Use of Synthetic “Internal Glycan” Vaccines - p. 334 22. Chapter Thirteen: Evaluation of Virus-Like Particle-Based Tumor-Associated Carbohydrate Immunogen in a Mouse Tumor Model - p. 357 23. Author Index - p. 377 24. Subject Index - p. 399
Chemical Glycobiology, Volume 597, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume, the first on chemical glycobiology, contains comprehensive chapters on the Discovery of New Glycosidases from Metagenomic Libraries, Structure-guided directed evolution of glycosidases: A case study in engineering a blood group antigen-cleaving enzyme, A Pipeline for Studying and Engineering Single-Subunit Oligosaccharyltransferases, Directed evolution of glycopeptides using mRNA display, Chemoenzymatic Synthesis and Applications of Prokaryote-Specific UDP-Sugars, and Biosynthesis of Legionaminic Acid and its Incorporation into Glycoconjugates.
Readers will find the latest information on this developing area of research, as reported by leaders in the field.
1. Cover image - p. iv 2. Title page - p. iv 3. Table of Contents - p. iv 4. Copyright - p. iv 5. Contributors - p. xi 6. Preface - p. xv 7. Section I: Evolution and Engineering of Glycans and Glycan Processing Enzymes - p. xix 8. Chapter One: Discovery of New Glycosidases From Metagenomic Libraries - p. xix 9. Chapter Two: Structure-Guided Directed Evolution of Glycosidases: A Case Study in Engineering a Blood Group Antigen-Cleaving Enzyme - p. 23 10. Chapter Three: A Pipeline for Studying and Engineering Single-Subunit Oligosaccharyltransferases - p. 53 11. Chapter Four: Directed Evolution of Glycopeptides Using mRNA Display - p. 81 12. Section II: Chemical and Enzymatic Synthesis and Analysis of Glycans and Glycoconjugates - p. 141 13. Chapter Five: Chemoenzymatic Synthesis and Applications of Prokaryote-Specific UDP-Sugars - p. 141 14. Chapter Six: Biosynthesis of Legionaminic Acid and Its Incorporation Into Glycoconjugates - p. 186 15. Chapter Seven: Profiling of Sugar Nucleotides - p. 207 16. Chapter Eight: Syntheses of Fluorescent Gangliosides for the Studies of Raft Domains - p. 238 17. Chapter Nine: Chemoenzymatic Glycan Remodeling of Natural and Recombinant Glycoproteins - p. 263 18. Section III: Glycan-Based Vaccines - p. 281 19. Chapter Ten: Glycoengineered Outer Membrane Vesicles as a Platform for Vaccine Development - p. 281 20. Chapter Eleven: Identification and Design of Synthetic B Cell Epitopes for Carbohydrate-Based Vaccines - p. 310 21. Chapter Twelve: Strategies in the Design and Use of Synthetic “Internal Glycan” Vaccines - p. 334 22. Chapter Thirteen: Evaluation of Virus-Like Particle-Based Tumor-Associated Carbohydrate Immunogen in a Mouse Tumor Model - p. 357 23. Author Index - p. 377 24. Subject Index - p. 399
