cryoEM, a new volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods and new developments in recording images, the creation, evaluation and validation of 3D maps from the images, model building into maps and refinement of the resulting atomic structures, and applications of essentially single particle methods to helical structures and to sub-tomogram averaging.
1. Cover image - p. iv 2. Title page - p. iv 3. Table of Contents - p. iv 4. Copyright - p. iv 5. Contributors - p. xi 6. Preface - p. xiii 7. Chapter One: Direct Electron Detectors - p. xx 8. Chapter Two: Specimen Behavior in the Electron Beam - p. 17 9. Chapter Three: Specimen Preparation for High-Resolution Cryo-EM - p. 50 10. Chapter Four: Strategies for Automated CryoEM Data Collection Using Direct Detectors - p. 86 11. Chapter Five: Processing of Cryo-EM Movie Data - p. 102 12. Chapter Six: Processing of Structurally Heterogeneous Cryo-EM Data in RELION - p. 124 13. Chapter Seven: Single-Particle Refinement and Variability Analysis in EMAN2.1 - p. 157 14. Chapter Eight: Frealign: An Exploratory Tool for Single-Particle Cryo-EM - p. 189 15. Chapter Nine: Testing the Validity of Single-Particle Maps at Low and High Resolution - p. 226 16. Chapter Ten: Tools for Model Building and Optimization into Near-Atomic Resolution Electron Cryo-Microscopy Density Maps - p. 253 17. Chapter Eleven: Refinement of Atomic Structures Against cryo-EM Maps - p. 276 18. Chapter Twelve: Cryo-EM Structure Determination Using Segmented Helical Image Reconstruction - p. 305 19. Chapter Thirteen: Cryo-Electron Tomography and Subtomogram Averaging - p. 328 20. Chapter Fourteen: High-Resolution Macromolecular Structure Determination by MicroED, a cryo-EM Method - p. 367 21. Chapter Fifteen: Databases and Archiving for CryoEM - p. 392 22. Author Index - p. 413 23. Subject Index - p. 431
cryoEM, a new volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods and new developments in recording images, the creation, evaluation and validation of 3D maps from the images, model building into maps and refinement of the resulting atomic structures, and applications of essentially single particle methods to helical structures and to sub-tomogram averaging.
1. Cover image - p. iv 2. Title page - p. iv 3. Table of Contents - p. iv 4. Copyright - p. iv 5. Contributors - p. xi 6. Preface - p. xiii 7. Chapter One: Direct Electron Detectors - p. xx 8. Chapter Two: Specimen Behavior in the Electron Beam - p. 17 9. Chapter Three: Specimen Preparation for High-Resolution Cryo-EM - p. 50 10. Chapter Four: Strategies for Automated CryoEM Data Collection Using Direct Detectors - p. 86 11. Chapter Five: Processing of Cryo-EM Movie Data - p. 102 12. Chapter Six: Processing of Structurally Heterogeneous Cryo-EM Data in RELION - p. 124 13. Chapter Seven: Single-Particle Refinement and Variability Analysis in EMAN2.1 - p. 157 14. Chapter Eight: Frealign: An Exploratory Tool for Single-Particle Cryo-EM - p. 189 15. Chapter Nine: Testing the Validity of Single-Particle Maps at Low and High Resolution - p. 226 16. Chapter Ten: Tools for Model Building and Optimization into Near-Atomic Resolution Electron Cryo-Microscopy Density Maps - p. 253 17. Chapter Eleven: Refinement of Atomic Structures Against cryo-EM Maps - p. 276 18. Chapter Twelve: Cryo-EM Structure Determination Using Segmented Helical Image Reconstruction - p. 305 19. Chapter Thirteen: Cryo-Electron Tomography and Subtomogram Averaging - p. 328 20. Chapter Fourteen: High-Resolution Macromolecular Structure Determination by MicroED, a cryo-EM Method - p. 367 21. Chapter Fifteen: Databases and Archiving for CryoEM - p. 392 22. Author Index - p. 413 23. Subject Index - p. 431
