Enzymatic Plastic Degradation, Volume 648 in the Methods in Enzymology series, continues the legacy of this premier serial with chapters authored by leaders in the field. Chapters in this latest release include Evaluating plastic pollution and environmental degradation, Assessment methods for microplastic pollution in the oceans and fresh water, Exploring microbial consortia from various environments for plastic degradation, Characterization of filamentous fungi for attack on synthetic polymers via biological Fenton chemistry, Synthesis of radioactive-labeled nanoplastics for assaying the environmental (microbial) PS degradation, Exploring metagenome for plastic degrading enzymes, Cutinases from thermophilic bacteria (actinomycetes): from identification to functional and structural characterization, and much more.
1. Cover image - p. page_i 2. Title page - p. page_i 3. Table of Contents - p. page_i 4. Copyright - p. page_iv 5. Contributors - p. page_xiii 6. Preface - p. page_xix 7. Chapter One: Current status and future development of plastics: Solutions for a circular economy and limitations of environmental degradation - p. page_1 8. Chapter Two: Methods for microplastic sampling and analysis in the seawater and fresh water environment - p. page_27 9. Chapter Three: Exploring microbial consortia from various environments for plastic degradation - p. page_47 10. Chapter Four: Cultivation of filamentous fungi for attack on synthetic polymers via biological Fenton chemistry - p. page_71 11. Chapter Five: Characterization of biodegradation of plastics in insect larvae - p. page_95 12. Chapter Six: Quantification of polystyrene plastics degradation using 14C isotope tracer technique - p. page_121 13. Chapter Seven: Exploring the global metagenome for plastic-degrading enzymes - p. page_137 14. Chapter Eight: Cutinases from thermophilic bacteria (actinomycetes): From identification to functional and structural characterization - p. page_159 15. Chapter Nine: Ideonella sakaiensis, PETase, and MHETase: From identification of microbial PET degradation to enzyme characterization - p. page_187 16. Chapter Ten: GRAPE, a greedy accumulated strategy for computational protein engineering - p. page_207 17. Chapter Eleven: Mechanistic investigation of enzymatic degradation of polyethylene terephthalate by nuclear magnetic resonance - p. page_231 18. Chapter Twelve: Fluorimetric high-throughput screening method for polyester hydrolase activity using polyethylene terephthalate nanoparticles - p. page_253 19. Chapter Thirteen: Anchor peptides promote degradation of mixed plastics for recycling - p. page_271 20. Chapter Fourteen: Tuning of adsorption of enzymes to polymer - p. page_293 21. Chapter Fifteen: Characterization of the enzymatic degradation of polyurethanes - p. page_317 22. Chapter Sixteen: Structural analysis of PET-degrading enzymes PETase and MHETase from Ideonella sakaiensis - p. page_337 23. Chapter Seventeen: Structural and functional characterization of nylon hydrolases - p. page_357 24. Chapter Eighteen: Upcycling of hydrolyzed PET by microbial conversion to a fatty acid derivative - p. page_391 25. Chapter Nineteen: Screening and cultivating microbial strains able to grow on building blocks of polyurethane - p. page_423 26. Chapter Twenty: Engineering microalgae as a whole cell catalyst for PET degradation - p. page_435 27. Chapter Twenty-One: Yeast cell surface display of bacterial PET hydrolase as a sustainable biocatalyst for the degradation of polyethylene terephthalate - p. page_457
Enzymatic Plastic Degradation, Volume 648 in the Methods in Enzymology series, continues the legacy of this premier serial with chapters authored by leaders in the field. Chapters in this latest release include Evaluating plastic pollution and environmental degradation, Assessment methods for microplastic pollution in the oceans and fresh water, Exploring microbial consortia from various environments for plastic degradation, Characterization of filamentous fungi for attack on synthetic polymers via biological Fenton chemistry, Synthesis of radioactive-labeled nanoplastics for assaying the environmental (microbial) PS degradation, Exploring metagenome for plastic degrading enzymes, Cutinases from thermophilic bacteria (actinomycetes): from identification to functional and structural characterization, and much more.
1. Cover image - p. page_i 2. Title page - p. page_i 3. Table of Contents - p. page_i 4. Copyright - p. page_iv 5. Contributors - p. page_xiii 6. Preface - p. page_xix 7. Chapter One: Current status and future development of plastics: Solutions for a circular economy and limitations of environmental degradation - p. page_1 8. Chapter Two: Methods for microplastic sampling and analysis in the seawater and fresh water environment - p. page_27 9. Chapter Three: Exploring microbial consortia from various environments for plastic degradation - p. page_47 10. Chapter Four: Cultivation of filamentous fungi for attack on synthetic polymers via biological Fenton chemistry - p. page_71 11. Chapter Five: Characterization of biodegradation of plastics in insect larvae - p. page_95 12. Chapter Six: Quantification of polystyrene plastics degradation using 14C isotope tracer technique - p. page_121 13. Chapter Seven: Exploring the global metagenome for plastic-degrading enzymes - p. page_137 14. Chapter Eight: Cutinases from thermophilic bacteria (actinomycetes): From identification to functional and structural characterization - p. page_159 15. Chapter Nine: Ideonella sakaiensis, PETase, and MHETase: From identification of microbial PET degradation to enzyme characterization - p. page_187 16. Chapter Ten: GRAPE, a greedy accumulated strategy for computational protein engineering - p. page_207 17. Chapter Eleven: Mechanistic investigation of enzymatic degradation of polyethylene terephthalate by nuclear magnetic resonance - p. page_231 18. Chapter Twelve: Fluorimetric high-throughput screening method for polyester hydrolase activity using polyethylene terephthalate nanoparticles - p. page_253 19. Chapter Thirteen: Anchor peptides promote degradation of mixed plastics for recycling - p. page_271 20. Chapter Fourteen: Tuning of adsorption of enzymes to polymer - p. page_293 21. Chapter Fifteen: Characterization of the enzymatic degradation of polyurethanes - p. page_317 22. Chapter Sixteen: Structural analysis of PET-degrading enzymes PETase and MHETase from Ideonella sakaiensis - p. page_337 23. Chapter Seventeen: Structural and functional characterization of nylon hydrolases - p. page_357 24. Chapter Eighteen: Upcycling of hydrolyzed PET by microbial conversion to a fatty acid derivative - p. page_391 25. Chapter Nineteen: Screening and cultivating microbial strains able to grow on building blocks of polyurethane - p. page_423 26. Chapter Twenty: Engineering microalgae as a whole cell catalyst for PET degradation - p. page_435 27. Chapter Twenty-One: Yeast cell surface display of bacterial PET hydrolase as a sustainable biocatalyst for the degradation of polyethylene terephthalate - p. page_457
