Modifications and Targeting of Protein Termini, Volume 684 in the Methods in Enzymology series serial highlights new advances in the field, with this new volume presenting interesting chapters pn a variety of timely topics, including Optimizing purification and activity assays of N-terminal methyltransferase complexes, In vitro reconstitution of hierarchical steps of Arg/N-degron pathway, Identification of N-degrons and N-recognins by using peptide-pull-downs combined with quantitative proteomics, A decoupled Virotrap approach to study the interactomes of N-terminal proteoforms, Monitoring ADO-dependent proteolysis in cells using fluorescent reporter proteins, Site-specific a-N-terminal methylation on peptides through chemical and enzymatic methods, and more.
Additional sections cover Monitoring the interactions between N-degrons and N-recognins of the Arg/N-degron pathway, Characterization and chemical modulation of p62/SQSTM1 as an autophagic N-recognin of the Arg/N-degron pathway, Analysis of higher plant N-degron pathway components and substrates via expression in S. cerevisiae, and so much more.
1. Cover image - p. i 2. Title page - p. ii 3. Table of Contents - p. iii 4. Series Page - p. iii 5. Copyright - p. iii 6. Contributors - p. iii 7. Preface: A nascent polypeptide emerges - p. xiii 8. Chapter One: Selective ribosome profiling as a tool to study interactions of translating ribosomes in mammalian cells - p. 22 9. Chapter Two: Deformylation of nascent peptide chains on the ribosome - p. 39 10. Chapter Three: Optimizing purification and activity assays of N-terminal methyltransferase complexes - p. 71 11. Chapter Four: Site-specific methylation on α-N-terminus of peptides through chemical and enzymatic methods - p. 113 12. Chapter Five: Biochemical and structural analysis of N-myristoyltransferase mediated protein tagging - p. 135 13. Chapter Six: Kinetic and catalytic features of N-myristoyltransferases - p. 167 14. Chapter Seven: Use of alkyne-tagged myristic acid to detect N-terminal myristoylation - p. 191 15. Chapter Eight: Peptide CoA conjugates for in situ proteomics profiling of acetyltransferase activities - p. 209 16. Chapter Nine: A decoupled Virotrap approach to study the interactomes of N-terminal proteoforms - p. 253 17. Chapter Ten: The amino-dipeptidyl peptidases DPP8 and DPP9: Purification and enzymatic assays - p. 289
Modifications and Targeting of Protein Termini, Volume 684 in the Methods in Enzymology series serial highlights new advances in the field, with this new volume presenting interesting chapters pn a variety of timely topics, including Optimizing purification and activity assays of N-terminal methyltransferase complexes, In vitro reconstitution of hierarchical steps of Arg/N-degron pathway, Identification of N-degrons and N-recognins by using peptide-pull-downs combined with quantitative proteomics, A decoupled Virotrap approach to study the interactomes of N-terminal proteoforms, Monitoring ADO-dependent proteolysis in cells using fluorescent reporter proteins, Site-specific a-N-terminal methylation on peptides through chemical and enzymatic methods, and more.
Additional sections cover Monitoring the interactions between N-degrons and N-recognins of the Arg/N-degron pathway, Characterization and chemical modulation of p62/SQSTM1 as an autophagic N-recognin of the Arg/N-degron pathway, Analysis of higher plant N-degron pathway components and substrates via expression in S. cerevisiae, and so much more.
1. Cover image - p. i 2. Title page - p. ii 3. Table of Contents - p. iii 4. Series Page - p. iii 5. Copyright - p. iii 6. Contributors - p. iii 7. Preface: A nascent polypeptide emerges - p. xiii 8. Chapter One: Selective ribosome profiling as a tool to study interactions of translating ribosomes in mammalian cells - p. 22 9. Chapter Two: Deformylation of nascent peptide chains on the ribosome - p. 39 10. Chapter Three: Optimizing purification and activity assays of N-terminal methyltransferase complexes - p. 71 11. Chapter Four: Site-specific methylation on α-N-terminus of peptides through chemical and enzymatic methods - p. 113 12. Chapter Five: Biochemical and structural analysis of N-myristoyltransferase mediated protein tagging - p. 135 13. Chapter Six: Kinetic and catalytic features of N-myristoyltransferases - p. 167 14. Chapter Seven: Use of alkyne-tagged myristic acid to detect N-terminal myristoylation - p. 191 15. Chapter Eight: Peptide CoA conjugates for in situ proteomics profiling of acetyltransferase activities - p. 209 16. Chapter Nine: A decoupled Virotrap approach to study the interactomes of N-terminal proteoforms - p. 253 17. Chapter Ten: The amino-dipeptidyl peptidases DPP8 and DPP9: Purification and enzymatic assays - p. 289
