Techniques for microfabricating intricate microfluidic structures that mimic the microenvironment of tissues and organs, combined with the development of biomaterials with carefully engineered surface properties, have enabled new paradigms in and cell culture-based models for human diseases. The dimensions of surface features and fluidic channels made accessible by these techniques are well-suited to the size scale of biological cells. Microfluidic Cell Culture Systems applies design and experimental techniques used in in microfluidics, and cell culture technologies to organ-on-chip systems.
This book is intended to serve as a professional reference, providing a practical guide to design and fabrication of microfluidic systems and biomaterials for use in cell culture systems and human organ models. The book covers topics ranging from academic first principles of microfluidic design, to clinical translation strategies for cell culture protocols. The goal is to help professionals coming from an engineering background to adapt their expertise for use in cell culture and organ models applications, and likewise to help biologists to design and employ microfluidic technologies in their cell culture systems.
This 2nd edition contains new material that strengthens the focus on in vitro models useful for drug discovery and development. One new chapter reviews liver organ models from an industry perspective, while others cover new technologies for scaling these models and for multi-organ systems. Other new chapters highlight the development of organ models and systems for specific applications in disease modeling and drug safety. Previous chapters have been revised to reflect the latest advances.
1. Cover image - p. iv 2. Title page - p. iv 3. Table of Contents - p. iv 4. Copyright - p. iv 5. Contributors - p. xiii 6. Chapter 1: Design principles for dynamic microphysiological systems - p. xv 7. Chapter 2: Microfluidic systems for controlling stem cell microenvironments - p. 29 8. Chapter 3: Microfluidic platforms with nanoscale features - p. 63 9. Chapter 4: Microfabricated kidney tissue models - p. 90 10. Chapter 5: Application of complex in vitro models (CIVMs) in drug discovery for safety testing and disease modeling - p. 120 11. Chapter 6: Hepatic microphysiological systems: Current and future applications in drug discovery and development - p. 158 12. Chapter 7: Microphysiological models of human organs: A case study on microengineered lung-on-a-chip systems - p. 186 13. Chapter 8: Cardiac tissue models - p. 208 14. Chapter 9: Neural tissue microphysiological systems in the era of patient-derived pluripotent stem cells - p. 248 15. Chapter 10: A high-throughput system to probe and direct biological functions driven by complex hemodynamic environments - p. 296 16. Chapter 11: Body-on-a-chip systems: Design, fabrication, and applications - p. 322 17. Chapter 12: On-chip disease models of the human retina - p. 350 18. Index - p. 373
Techniques for microfabricating intricate microfluidic structures that mimic the microenvironment of tissues and organs, combined with the development of biomaterials with carefully engineered surface properties, have enabled new paradigms in and cell culture-based models for human diseases. The dimensions of surface features and fluidic channels made accessible by these techniques are well-suited to the size scale of biological cells. Microfluidic Cell Culture Systems applies design and experimental techniques used in in microfluidics, and cell culture technologies to organ-on-chip systems.
This book is intended to serve as a professional reference, providing a practical guide to design and fabrication of microfluidic systems and biomaterials for use in cell culture systems and human organ models. The book covers topics ranging from academic first principles of microfluidic design, to clinical translation strategies for cell culture protocols. The goal is to help professionals coming from an engineering background to adapt their expertise for use in cell culture and organ models applications, and likewise to help biologists to design and employ microfluidic technologies in their cell culture systems.
This 2nd edition contains new material that strengthens the focus on in vitro models useful for drug discovery and development. One new chapter reviews liver organ models from an industry perspective, while others cover new technologies for scaling these models and for multi-organ systems. Other new chapters highlight the development of organ models and systems for specific applications in disease modeling and drug safety. Previous chapters have been revised to reflect the latest advances.
1. Cover image - p. iv 2. Title page - p. iv 3. Table of Contents - p. iv 4. Copyright - p. iv 5. Contributors - p. xiii 6. Chapter 1: Design principles for dynamic microphysiological systems - p. xv 7. Chapter 2: Microfluidic systems for controlling stem cell microenvironments - p. 29 8. Chapter 3: Microfluidic platforms with nanoscale features - p. 63 9. Chapter 4: Microfabricated kidney tissue models - p. 90 10. Chapter 5: Application of complex in vitro models (CIVMs) in drug discovery for safety testing and disease modeling - p. 120 11. Chapter 6: Hepatic microphysiological systems: Current and future applications in drug discovery and development - p. 158 12. Chapter 7: Microphysiological models of human organs: A case study on microengineered lung-on-a-chip systems - p. 186 13. Chapter 8: Cardiac tissue models - p. 208 14. Chapter 9: Neural tissue microphysiological systems in the era of patient-derived pluripotent stem cells - p. 248 15. Chapter 10: A high-throughput system to probe and direct biological functions driven by complex hemodynamic environments - p. 296 16. Chapter 11: Body-on-a-chip systems: Design, fabrication, and applications - p. 322 17. Chapter 12: On-chip disease models of the human retina - p. 350 18. Index - p. 373
