Exosome Communication: Advances in Research and Therapeutics for Health and Disease serves as a crucial reference for pharmaceutical scientists, focusing on the key qualities of exosomes as drug delivery vehicles. Exosomes are intracellular membrane-based vesicles with diverse compositions, offering significant advantages over traditional drug delivery systems such as liposomes and polymeric nanoparticles, due to their nonimmunogenic nature. This book provides a comprehensive exploration of exosomes, starting with their history and development, understanding extracellular vesicles and biogenesis, and techniques for isolation and characterization. This book also addresses critical considerations like quality control, heterogeneity reduction, regulatory and ethical aspects, clinical trials, and scale-up manufacturing.
1. Chapter 1 Introduction: History and development of exosomes nanovesicles - p. 1 2. Chapter 2 Exploring extracellular vesicles: Understanding exosomes and beyond - p. 25 3. Chapter 3 Exosome isolation techniques: Methods, protocols, and best practices - p. 59 4. Chapter 4 Improving cargo loading and release strategies for exosomes nanovesicles - p. 83 5. Chapter 5 Exosome characterization techniques, composition analysis and contents of exosomes nanovesicles: Methods and interpretation - p. 105 6. Chapter 6 Quality control, minimizing heterogeneity and enhancing yield in exosome isolation and production - p. 129 7. Chapter 7 Inhibition of cell-cell communication in exosomes - p. 149 8. Chapter 8 Bioengineering and modification of exosome nanovesicles for targeted drug delivery - p. 167 9. Chapter 9 Challenges in exosome nanovesicle-based drug delivery and diagnostics - p. 203 10. Chapter 10 Diagnostic potential and biomarkers potential of exosome nanovesicles - p. 253 11. Chapter 11 Immunological exosomes: An upcoming cue for disease management - p. 285 12. Chapter 12 Exosomes for protein and peptide drug delivery - p. 305 13. Chapter 13 Exosomes nanovesicles for gene delivery and vaccination - p. 329 14. Chapter 14 Theranostic potential of exosomes in neurodegenerative diseases - p. 375 15. Chapter 15 Exosomes in cancer therapeutic delivery - p. 393 16. Chapter 16 Potential role of parasite-derived exosomes in human diseases - p. 421 17. Chapter 17 Current applications of new generations of exosomes nanovesicles - p. 445 18. Chapter 18 Clinical trials and translational research in exosome nanovesicle therapeutics - p. 473 19. Chapter 19 Scale-up and manufacturing of exosome-based therapeutics - p. 503 20. Chapter 20 Regulatory and ethical considerations in exosome-based therapeutics - p. 523 21. 20.1: Introduction - p. 523 22. 20.2: Key considerations for developing exosome-based therapeutics - p. 526 23. 20.3: Ethical challenges associated with exosome therapy - p. 527 24. 20.4: Current understanding in regulatory and ethical framework for exosome-based therapeutics - p. 528 25. 20.5: GMP-grade exosome production methods - p. 535 26. 20.6: Exosome therapeutics: Preclinical and clinical considerations - p. 538 27. 20.7: Regulatory and ethical issues in exosome therapeutics: Overcoming technical and biological obstacles - p. 541 28. 20.8: Discussion - p. 545 29. 20.9: Conclusion - p. 546 30. Index - p. 553
Exosome Communication: Advances in Research and Therapeutics for Health and Disease serves as a crucial reference for pharmaceutical scientists, focusing on the key qualities of exosomes as drug delivery vehicles. Exosomes are intracellular membrane-based vesicles with diverse compositions, offering significant advantages over traditional drug delivery systems such as liposomes and polymeric nanoparticles, due to their nonimmunogenic nature. This book provides a comprehensive exploration of exosomes, starting with their history and development, understanding extracellular vesicles and biogenesis, and techniques for isolation and characterization. This book also addresses critical considerations like quality control, heterogeneity reduction, regulatory and ethical aspects, clinical trials, and scale-up manufacturing.
1. Chapter 1 Introduction: History and development of exosomes nanovesicles - p. 1 2. Chapter 2 Exploring extracellular vesicles: Understanding exosomes and beyond - p. 25 3. Chapter 3 Exosome isolation techniques: Methods, protocols, and best practices - p. 59 4. Chapter 4 Improving cargo loading and release strategies for exosomes nanovesicles - p. 83 5. Chapter 5 Exosome characterization techniques, composition analysis and contents of exosomes nanovesicles: Methods and interpretation - p. 105 6. Chapter 6 Quality control, minimizing heterogeneity and enhancing yield in exosome isolation and production - p. 129 7. Chapter 7 Inhibition of cell-cell communication in exosomes - p. 149 8. Chapter 8 Bioengineering and modification of exosome nanovesicles for targeted drug delivery - p. 167 9. Chapter 9 Challenges in exosome nanovesicle-based drug delivery and diagnostics - p. 203 10. Chapter 10 Diagnostic potential and biomarkers potential of exosome nanovesicles - p. 253 11. Chapter 11 Immunological exosomes: An upcoming cue for disease management - p. 285 12. Chapter 12 Exosomes for protein and peptide drug delivery - p. 305 13. Chapter 13 Exosomes nanovesicles for gene delivery and vaccination - p. 329 14. Chapter 14 Theranostic potential of exosomes in neurodegenerative diseases - p. 375 15. Chapter 15 Exosomes in cancer therapeutic delivery - p. 393 16. Chapter 16 Potential role of parasite-derived exosomes in human diseases - p. 421 17. Chapter 17 Current applications of new generations of exosomes nanovesicles - p. 445 18. Chapter 18 Clinical trials and translational research in exosome nanovesicle therapeutics - p. 473 19. Chapter 19 Scale-up and manufacturing of exosome-based therapeutics - p. 503 20. Chapter 20 Regulatory and ethical considerations in exosome-based therapeutics - p. 523 21. 20.1: Introduction - p. 523 22. 20.2: Key considerations for developing exosome-based therapeutics - p. 526 23. 20.3: Ethical challenges associated with exosome therapy - p. 527 24. 20.4: Current understanding in regulatory and ethical framework for exosome-based therapeutics - p. 528 25. 20.5: GMP-grade exosome production methods - p. 535 26. 20.6: Exosome therapeutics: Preclinical and clinical considerations - p. 538 27. 20.7: Regulatory and ethical issues in exosome therapeutics: Overcoming technical and biological obstacles - p. 541 28. 20.8: Discussion - p. 545 29. 20.9: Conclusion - p. 546 30. Index - p. 553
