Genome Editing: A Practical Guide to Research and Clinical Applications is geared towards investigators interested in learning how to use CRISPR-Cas9-based technologies, with a focus on cardiovascular research and clinical applications. Covering a range of topics from the basics of genome editing to design considerations, to assessments and applications, this reference allows readers to get started and establish a full workflow from the beginning of the project to its full completion. With worked examples drawn from real-life experiments, as well as troubleshooting and pitfalls to avoid, the book serves as an essential reference for researchers and investigators in both cardiovascular and biomedical research.
1. Genome Editing - p. Page i 2. Cover - p. Page i 3. Title page - p. Page ii 4. Table of Contents - p. Page iii 5. Copyright - p. Page iii 6. Dedication - p. Page v 7. Contents - p. Page vii 8. Preface - p. Page vii 9. List of Illustrations - p. Page x 10. Chapter 1 : A brief history and primer on genome editing - p. Page x 11. Chapter 2 : Choosing a genome editing strategy and target site - p. Page 16 12. Chapter 3 : Choosing a nuclease, guide RNA, and repair template - p. Page 38 13. Chapter 4 : Assessing the outcomes of genome editing - p. Page 58 14. Chapter 5 : Assessing for off-target mutagenesis - p. Page 80 15. Chapter 6 : Base editing - p. Page 98 16. Chapter 7 : Alternative types of editing - p. Page 119 17. Chapter 8 : Genome editing for cellular disease modeling - p. Page 141 18. Chapter 9 : Genome editing for functional experiments and screens - p. Page 166 19. Chapter 10 : Therapeutic genome editing - p. Page 190 20. Index - p. Page 209
Genome Editing: A Practical Guide to Research and Clinical Applications is geared towards investigators interested in learning how to use CRISPR-Cas9-based technologies, with a focus on cardiovascular research and clinical applications. Covering a range of topics from the basics of genome editing to design considerations, to assessments and applications, this reference allows readers to get started and establish a full workflow from the beginning of the project to its full completion. With worked examples drawn from real-life experiments, as well as troubleshooting and pitfalls to avoid, the book serves as an essential reference for researchers and investigators in both cardiovascular and biomedical research.
1. Genome Editing - p. Page i 2. Cover - p. Page i 3. Title page - p. Page ii 4. Table of Contents - p. Page iii 5. Copyright - p. Page iii 6. Dedication - p. Page v 7. Contents - p. Page vii 8. Preface - p. Page vii 9. List of Illustrations - p. Page x 10. Chapter 1 : A brief history and primer on genome editing - p. Page x 11. Chapter 2 : Choosing a genome editing strategy and target site - p. Page 16 12. Chapter 3 : Choosing a nuclease, guide RNA, and repair template - p. Page 38 13. Chapter 4 : Assessing the outcomes of genome editing - p. Page 58 14. Chapter 5 : Assessing for off-target mutagenesis - p. Page 80 15. Chapter 6 : Base editing - p. Page 98 16. Chapter 7 : Alternative types of editing - p. Page 119 17. Chapter 8 : Genome editing for cellular disease modeling - p. Page 141 18. Chapter 9 : Genome editing for functional experiments and screens - p. Page 166 19. Chapter 10 : Therapeutic genome editing - p. Page 190 20. Index - p. Page 209
