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Modern neuroscience research is inherently multidisciplinary, with a wide variety of cutting edge new techniques to explore multiple levels of investigation. This Third Edition of Guide to Research Techniques in Neuroscience provides a comprehensive overview of classical and cutting edge methods including their utility, limitations, and how data are presented in the literature. This book can be used as an introduction to neuroscience techniques for anyone new to the field or as a reference for any neuroscientist while reading papers or attending talks.

  • Nearly 200 updated full-color illustrations to clearly convey the theory and practice of neuroscience methods
  • Expands on techniques from previous editions and covers many new techniques including in vivo calcium imaging, fiber photometry, RNA-Seq, brain spheroids, CRISPR-Cas9 genome editing, and more
  • Clear, straightforward explanations of each technique for anyone new to the field
  • A broad scope of methods, from noninvasive brain imaging in human subjects, to electrophysiology in animal models, to recombinant DNA technology in test tubes, to transfection of neurons in cell culture
  • Detailed recommendations on where to find protocols and other resources for specific techniques
  • "Walk-through" boxes that guide readers through experiments step-by-step

1. Introduction - p. xv 2. Chapter 1. Noninvasive Brain Imaging - p. 1 3. Chapter 2. Animal Behavior - p. 39 4. Chapter 3. Stereotaxic Surgeries - p. 73 5. Chapter 4. Electrophysiology - p. 85 6. Chapter 5. Microscopy - p. 115 7. Chapter 6. Visualizing Nervous System Structure - p. 145 8. Chapter 7. Visualizing and Measuring Neural Dynamics - p. 169 9. Chapter 8. Manipulating Neural Activity - p. 191 10. Chapter 9. Identifying Genes and Proteins of Interest - p. 209 11. Chapter 10. Molecular Cloning and Recombinant DNA Technology - p. 227 12. Chapter 11. Gene Delivery Strategies - p. 245 13. Chapter 12. Making and Using Genetically Modified Organisms - p. 259 14. Chapter 13. Cell Culture Techniques - p. 291 15. Chapter 14. Intracellular Signaling and Biochemical Assays - p. 309 16. Glossary - p. 349 17. Index - p. 377

Descripcion
Descripción

Modern neuroscience research is inherently multidisciplinary, with a wide variety of cutting edge new techniques to explore multiple levels of investigation. This Third Edition of Guide to Research Techniques in Neuroscience provides a comprehensive overview of classical and cutting edge methods including their utility, limitations, and how data are presented in the literature. This book can be used as an introduction to neuroscience techniques for anyone new to the field or as a reference for any neuroscientist while reading papers or attending talks.

  • Nearly 200 updated full-color illustrations to clearly convey the theory and practice of neuroscience methods
  • Expands on techniques from previous editions and covers many new techniques including in vivo calcium imaging, fiber photometry, RNA-Seq, brain spheroids, CRISPR-Cas9 genome editing, and more
  • Clear, straightforward explanations of each technique for anyone new to the field
  • A broad scope of methods, from noninvasive brain imaging in human subjects, to electrophysiology in animal models, to recombinant DNA technology in test tubes, to transfection of neurons in cell culture
  • Detailed recommendations on where to find protocols and other resources for specific techniques
  • "Walk-through" boxes that guide readers through experiments step-by-step

1. Introduction - p. xv 2. Chapter 1. Noninvasive Brain Imaging - p. 1 3. Chapter 2. Animal Behavior - p. 39 4. Chapter 3. Stereotaxic Surgeries - p. 73 5. Chapter 4. Electrophysiology - p. 85 6. Chapter 5. Microscopy - p. 115 7. Chapter 6. Visualizing Nervous System Structure - p. 145 8. Chapter 7. Visualizing and Measuring Neural Dynamics - p. 169 9. Chapter 8. Manipulating Neural Activity - p. 191 10. Chapter 9. Identifying Genes and Proteins of Interest - p. 209 11. Chapter 10. Molecular Cloning and Recombinant DNA Technology - p. 227 12. Chapter 11. Gene Delivery Strategies - p. 245 13. Chapter 12. Making and Using Genetically Modified Organisms - p. 259 14. Chapter 13. Cell Culture Techniques - p. 291 15. Chapter 14. Intracellular Signaling and Biochemical Assays - p. 309 16. Glossary - p. 349 17. Index - p. 377

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