This volume in the well-established Methods in Enzymology series features methods for the study of lipids using mass spectrometry techniques. Articles in this volume cover topics such as Qualitative Analysis and Quantitative Assessment of Changes in Neutral Glycerol Lipid Molecular Species within Cells; Glycerophospholipid identification and quantitation by electrospray ionization mass spectrometry; Detection and Quantitation of Eicosanoids via High Performance Liquid Chromatography/Electrospray Ionization Mass Spectrometry; Structure-specific, quantitative methods for "lipidomic" analysis of sphingolipids by tandem mass spectrometry; Analysis of Ubiquinones, Dolichols and Dolichol Diphosphate-Oligosaccharides by Liquid Chromatography Electrospray Ionization Mass Spectrometry; Extraction and Analysis of Sterols in Biological Matrices by High-Performance Liquid Chromatography Electrospray Ionization Mass Spectrometry; The Lipid Maps Initiative in Lipidomics; Basic analytical systems for lipidomics by mass spectrometry in Japan; The European Lipidomics Initiative Enabling technologies; Lipidomic analysis of Signaling Pathways; Bioinformatics for Lipidomics; Mediator Lipidomics: Search Algorithms for Eicosanoids, Resolvins and Protectins; A guide to biochemical systems modeling of sphingolipids for the biochemist; and Quantitation and Standardization of Lipid Internal Standards for Mass Spectroscopy.
1. Cover image 2. Title page 3. Table of Contents 4. Qualitative Analysis and Quantitative Assessment of Changes in Neutral Glycerol Lipid Molecular Species Within Cells 5. Glycerophospholipid Identification and Quantitation by Electrospray Ionization Mass Spectrometry 6. Detection and Quantitation of Eicosanoids via High Performance Liquid Chromatography–Electrospray Ionization–Mass Spectrometry 7. Structure–Specific, Quantitative Methods for Analysis of Sphingolipids by Liquid Chromatography–Tandem Mass Spectrometry: “Inside–Out” Sphingolipidomics 8. Analysis of Ubiquinones, Dolichols, and Dolichol Diphosphate–Oligosaccharides by Liquid Chromatography–Electrospray Ionization–Mass Spectrometry 9. Extraction and Analysis of Sterols in Biological Matrices by High Performance Liquid Chromatography Electrospray Ionization Mass Spectrometry 10. The Lipid Maps Initiative in Lipidomics 11. Basic Analytical Systems for Lipidomics by Mass Spectrometry in Japan 12. The European Lipidomics Initiative: Enabling Technologies 13. Lipidomic Analysis of Signaling Pathways 14. Bioinformatics for Lipidomics 15. Mediator Lipidomics: Search Algorithms for Eicosanoids, Resolvins, and Protectins 16. A Guide to Biochemical Systems Modeling of Sphingolipids for the Biochemist 17. Quantitation and Standardization of Lipid Internal Standards for Mass Spectroscopy 18. Contributors to Volume 432 19. Preface 20. Author Index 21. Contents of Previous Volumes 22. Subject Index
This volume in the well-established Methods in Enzymology series features methods for the study of lipids using mass spectrometry techniques. Articles in this volume cover topics such as Qualitative Analysis and Quantitative Assessment of Changes in Neutral Glycerol Lipid Molecular Species within Cells; Glycerophospholipid identification and quantitation by electrospray ionization mass spectrometry; Detection and Quantitation of Eicosanoids via High Performance Liquid Chromatography/Electrospray Ionization Mass Spectrometry; Structure-specific, quantitative methods for "lipidomic" analysis of sphingolipids by tandem mass spectrometry; Analysis of Ubiquinones, Dolichols and Dolichol Diphosphate-Oligosaccharides by Liquid Chromatography Electrospray Ionization Mass Spectrometry; Extraction and Analysis of Sterols in Biological Matrices by High-Performance Liquid Chromatography Electrospray Ionization Mass Spectrometry; The Lipid Maps Initiative in Lipidomics; Basic analytical systems for lipidomics by mass spectrometry in Japan; The European Lipidomics Initiative Enabling technologies; Lipidomic analysis of Signaling Pathways; Bioinformatics for Lipidomics; Mediator Lipidomics: Search Algorithms for Eicosanoids, Resolvins and Protectins; A guide to biochemical systems modeling of sphingolipids for the biochemist; and Quantitation and Standardization of Lipid Internal Standards for Mass Spectroscopy.
1. Cover image 2. Title page 3. Table of Contents 4. Qualitative Analysis and Quantitative Assessment of Changes in Neutral Glycerol Lipid Molecular Species Within Cells 5. Glycerophospholipid Identification and Quantitation by Electrospray Ionization Mass Spectrometry 6. Detection and Quantitation of Eicosanoids via High Performance Liquid Chromatography–Electrospray Ionization–Mass Spectrometry 7. Structure–Specific, Quantitative Methods for Analysis of Sphingolipids by Liquid Chromatography–Tandem Mass Spectrometry: “Inside–Out” Sphingolipidomics 8. Analysis of Ubiquinones, Dolichols, and Dolichol Diphosphate–Oligosaccharides by Liquid Chromatography–Electrospray Ionization–Mass Spectrometry 9. Extraction and Analysis of Sterols in Biological Matrices by High Performance Liquid Chromatography Electrospray Ionization Mass Spectrometry 10. The Lipid Maps Initiative in Lipidomics 11. Basic Analytical Systems for Lipidomics by Mass Spectrometry in Japan 12. The European Lipidomics Initiative: Enabling Technologies 13. Lipidomic Analysis of Signaling Pathways 14. Bioinformatics for Lipidomics 15. Mediator Lipidomics: Search Algorithms for Eicosanoids, Resolvins, and Protectins 16. A Guide to Biochemical Systems Modeling of Sphingolipids for the Biochemist 17. Quantitation and Standardization of Lipid Internal Standards for Mass Spectroscopy 18. Contributors to Volume 432 19. Preface 20. Author Index 21. Contents of Previous Volumes 22. Subject Index
