Cytokinesis, the latest volume in the Methods in Cell Biology series, looks at the latest advances in cytokinesis. Edited by leaders in the field, this volume presents proven, state-of-art techniques, along with relevant historical background and theory, to aid researchers in efficient design and effective implementation of experimental methodologies.
1. Cover image - p. i 2. Title page - p. i 3. Table of Contents - p. iii 4. Series Editors - p. ii 5. Copyright - p. iv 6. Contributors - p. xiii 7. Preface - p. xix 8. Chapter 1. Labeling membrane domains in dividing echinoderm eggs - p. 1 9. Chapter 2. Dynamics of sphingomyelin- and cholesterol-enriched lipid domains during cytokinesis - p. 15 10. Chapter 3. Analysis of protein dynamics during cytokinesis in budding yeast - p. 25 11. Chapter 4. Imaging cytokinesis of Drosophila S2 cells - p. 47 12. Chapter 5. Studying cytokinesis in Drosophila epithelial tissues - p. 73 13. Chapter 6. Visualization of cleavage furrow proteins in fixed dividing spermatocytes - p. 85 14. Chapter 7. Monitoring complete and incomplete abscission in the germ line stem cell lineage of Drosophila ovaries - p. 105 15. Chapter 8. Incomplete cytokinesis/binucleation in mammals: The powerful system of hepatocytes - p. 119 16. Chapter 9. Specific membrane dynamics during neural stem cell division - p. 143 17. Chapter 10. Understanding post-mitotic roles of the midbody during cell differentiation and polarization - p. 173 18. Chapter 11. Micromanipulation of daughter cells for the study of cytokinetic abscission - p. 187 19. Chapter 12. Measuring abscission spatiotemporal dynamics using quantitative high-resolution microscopy - p. 205 20. Chapter 13. Analysis of cytokinesis by electron microscopy - p. 225 21. Chapter 14. Studying cytokinesis and midbody remnants using correlative light/scanning EM - p. 239 22. Chapter 15. Analysis of postcytokinetic roles of cytokinetic components in Caenorhabditis elegans - p. 253 23. Chapter 16. Variations on a theme: Imaging cytokinetic and stable rings in situ using Caenorhabditis elegans - p. 267 24. Chapter 17. Using fast-acting temperature-sensitive mutants to study cell division in Caenorhabditis elegans - p. 283 25. Chapter 18. Cytokinesis from nanometers to micrometers and microseconds to minutes - p. 307 26. Chapter 19. An active contour ImageJ plugin to monitor daughter cell size in 3D during cytokinesis - p. 323 27. Chapter 20. Nuclear displacement and fluorescence recovery after photobleaching (FRAP) assays to study division site placement and cytokinesis in fission yeast - p. 341 28. Chapter 21. Single-molecule measurements to study polymerization dynamics of FtsZ-FtsA copolymers - p. 355 29. Chapter 22. Preparation of centralspindlin as an active heterotetramer of kinesin and GTPase activating protein subunits for in vitro structural and functional assays - p. 371 30. Chapter 23. In vitro reactivation of the cytokinetic contractile ring of fission yeast cells - p. 387 31. Chapter 24. Xenopus extract approaches to studying microtubule organization and signaling in cytokinesis - p. 395 32. Index - p. 437 33. Volumes in Series - p. 447
Cytokinesis, the latest volume in the Methods in Cell Biology series, looks at the latest advances in cytokinesis. Edited by leaders in the field, this volume presents proven, state-of-art techniques, along with relevant historical background and theory, to aid researchers in efficient design and effective implementation of experimental methodologies.
1. Cover image - p. i 2. Title page - p. i 3. Table of Contents - p. iii 4. Series Editors - p. ii 5. Copyright - p. iv 6. Contributors - p. xiii 7. Preface - p. xix 8. Chapter 1. Labeling membrane domains in dividing echinoderm eggs - p. 1 9. Chapter 2. Dynamics of sphingomyelin- and cholesterol-enriched lipid domains during cytokinesis - p. 15 10. Chapter 3. Analysis of protein dynamics during cytokinesis in budding yeast - p. 25 11. Chapter 4. Imaging cytokinesis of Drosophila S2 cells - p. 47 12. Chapter 5. Studying cytokinesis in Drosophila epithelial tissues - p. 73 13. Chapter 6. Visualization of cleavage furrow proteins in fixed dividing spermatocytes - p. 85 14. Chapter 7. Monitoring complete and incomplete abscission in the germ line stem cell lineage of Drosophila ovaries - p. 105 15. Chapter 8. Incomplete cytokinesis/binucleation in mammals: The powerful system of hepatocytes - p. 119 16. Chapter 9. Specific membrane dynamics during neural stem cell division - p. 143 17. Chapter 10. Understanding post-mitotic roles of the midbody during cell differentiation and polarization - p. 173 18. Chapter 11. Micromanipulation of daughter cells for the study of cytokinetic abscission - p. 187 19. Chapter 12. Measuring abscission spatiotemporal dynamics using quantitative high-resolution microscopy - p. 205 20. Chapter 13. Analysis of cytokinesis by electron microscopy - p. 225 21. Chapter 14. Studying cytokinesis and midbody remnants using correlative light/scanning EM - p. 239 22. Chapter 15. Analysis of postcytokinetic roles of cytokinetic components in Caenorhabditis elegans - p. 253 23. Chapter 16. Variations on a theme: Imaging cytokinetic and stable rings in situ using Caenorhabditis elegans - p. 267 24. Chapter 17. Using fast-acting temperature-sensitive mutants to study cell division in Caenorhabditis elegans - p. 283 25. Chapter 18. Cytokinesis from nanometers to micrometers and microseconds to minutes - p. 307 26. Chapter 19. An active contour ImageJ plugin to monitor daughter cell size in 3D during cytokinesis - p. 323 27. Chapter 20. Nuclear displacement and fluorescence recovery after photobleaching (FRAP) assays to study division site placement and cytokinesis in fission yeast - p. 341 28. Chapter 21. Single-molecule measurements to study polymerization dynamics of FtsZ-FtsA copolymers - p. 355 29. Chapter 22. Preparation of centralspindlin as an active heterotetramer of kinesin and GTPase activating protein subunits for in vitro structural and functional assays - p. 371 30. Chapter 23. In vitro reactivation of the cytokinetic contractile ring of fission yeast cells - p. 387 31. Chapter 24. Xenopus extract approaches to studying microtubule organization and signaling in cytokinesis - p. 395 32. Index - p. 437 33. Volumes in Series - p. 447
