This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers G protein coupled receptors, and includes chapters on such topics as GPCR modelling, interactions with other molecules, virtual screening and GPCR activation.
1. Cover image 2. Title page 3. Table of Contents 4. Copyright 5. Contributors 6. Preface 7. Methods in Enzymology 8. Chapter One. The Protein Local Optimization Program and G-Protein-Coupled Receptors: Loop Restoration and Applications to Homology Modeling 9. Chapter Two. Modeling Active GPCR Conformations 10. Chapter Three. Molecular Modeling of Adenosine Receptors 11. Chapter Four. Predicting G-Protein-Coupled Receptors Families Using Different Physiochemical Properties and Pseudo Amino Acid Composition 12. Chapter Five. Computational Design and Experimental Characterization of GPCR Segment Models 13. Chapter Six. G-Protein-Coupled Receptor Interaction with Small GTPases 14. Chapter Seven. Quantification of Receptor Tyrosine Kinase Activation and Transactivation by G-Protein-Coupled Receptors Using Spatial Intensity Distribution Analysis (SpIDA) 15. Chapter Eight. NMR Methods for Detection of Small Molecule Binding to RGS4 16. Chapter Nine. Group II Activators of G-protein Signaling: Monitoring the Interaction of Ga with the G-protein Regulatory Motif in the Intact Cell 17. Chapter Ten. Homogeneous Time-Resolved Fluorescence Assay to Probe Folded G Protein-Coupled Receptors 18. Chapter Eleven. cAMP Biosensors Applied in Molecular Pharmacological Studies of G Protein-Coupled Receptors 19. Chapter Twelve. Rhodopsin–Lipid Interactions Studied by NMR 20. Chapter Thirteen. Biasing the Parathyroid Hormone Receptor: Relating In Vitro Ligand Efficacy to In Vivo Biological Activity 21. Chapter Fourteen. Application of Monte Carlo-Based Receptor Ensemble Docking to Virtual Screening for GPCR Ligands 22. Chapter Fifteen. From Heptahelical Bundle to Hits from the Haystack 23. Chapter Sixteen. Kinetics and Dynamics in the G Protein-Coupled Receptor Signaling Cascade 24. Chapter Seventeen. Magic Angle Spinning Nuclear Magnetic Resonance Spectroscopy of G Protein-Coupled Receptors 25. Chapter Eighteen. Posttranslation Modification of G Protein-Coupled Receptor in Relationship to Biased Agonism 26. Chapter Nineteen. Protein S-Nitrosylation Measurement 27. Author Index 28. Subject Index
This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers G protein coupled receptors, and includes chapters on such topics as GPCR modelling, interactions with other molecules, virtual screening and GPCR activation.
1. Cover image 2. Title page 3. Table of Contents 4. Copyright 5. Contributors 6. Preface 7. Methods in Enzymology 8. Chapter One. The Protein Local Optimization Program and G-Protein-Coupled Receptors: Loop Restoration and Applications to Homology Modeling 9. Chapter Two. Modeling Active GPCR Conformations 10. Chapter Three. Molecular Modeling of Adenosine Receptors 11. Chapter Four. Predicting G-Protein-Coupled Receptors Families Using Different Physiochemical Properties and Pseudo Amino Acid Composition 12. Chapter Five. Computational Design and Experimental Characterization of GPCR Segment Models 13. Chapter Six. G-Protein-Coupled Receptor Interaction with Small GTPases 14. Chapter Seven. Quantification of Receptor Tyrosine Kinase Activation and Transactivation by G-Protein-Coupled Receptors Using Spatial Intensity Distribution Analysis (SpIDA) 15. Chapter Eight. NMR Methods for Detection of Small Molecule Binding to RGS4 16. Chapter Nine. Group II Activators of G-protein Signaling: Monitoring the Interaction of Ga with the G-protein Regulatory Motif in the Intact Cell 17. Chapter Ten. Homogeneous Time-Resolved Fluorescence Assay to Probe Folded G Protein-Coupled Receptors 18. Chapter Eleven. cAMP Biosensors Applied in Molecular Pharmacological Studies of G Protein-Coupled Receptors 19. Chapter Twelve. Rhodopsin–Lipid Interactions Studied by NMR 20. Chapter Thirteen. Biasing the Parathyroid Hormone Receptor: Relating In Vitro Ligand Efficacy to In Vivo Biological Activity 21. Chapter Fourteen. Application of Monte Carlo-Based Receptor Ensemble Docking to Virtual Screening for GPCR Ligands 22. Chapter Fifteen. From Heptahelical Bundle to Hits from the Haystack 23. Chapter Sixteen. Kinetics and Dynamics in the G Protein-Coupled Receptor Signaling Cascade 24. Chapter Seventeen. Magic Angle Spinning Nuclear Magnetic Resonance Spectroscopy of G Protein-Coupled Receptors 25. Chapter Eighteen. Posttranslation Modification of G Protein-Coupled Receptor in Relationship to Biased Agonism 26. Chapter Nineteen. Protein S-Nitrosylation Measurement 27. Author Index 28. Subject Index
