Single-Molecule Enzymology, Part B, the latest volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in single-molecule enzymology, and includes sections on such topics as force-based and hybrid approaches, fluorescence, high-throughput sm enzymology, and nanopore and tethered particle motion.
1. Cover image - p. iv 2. Title page - p. iv 3. Table of Contents - p. iv 4. Copyright - p. iv 5. Contributors - p. xi 6. Preface - p. xv 7. Chapter One: How to Measure Load-Dependent Kinetics of Individual Motor Molecules Without a Force-Clamp - p. xvi 8. Chapter Two: Studying the Mechanochemistry of Processive Cytoskeletal Motors With an Optical Trap - p. 29 9. Chapter Three: Single-Molecule Optical-Trapping Techniques to Study Molecular Mechanisms of a Replisome - p. 54 10. Chapter Four: Recent Advances in Biological Single-Molecule Applications of Optical Tweezers and Fluorescence Microscopy - p. 84 11. Chapter Five: Direct Visualization of Helicase Dynamics Using Fluorescence Localization and Optical Trapping - p. 119 12. Chapter Six: High-Resolution Optical Tweezers Combined With Single-Molecule Confocal Microscopy - p. 136 13. Chapter Seven: Integrating Optical Tweezers, DNA Tightropes, and Single-Molecule Fluorescence Imaging: Pitfalls and Traps - p. 169 14. Chapter Eight: Single-Stranded DNA Curtains for Studying Homologous Recombination - p. 192 15. Chapter Nine: Inserting Extrahelical Structures into Long DNA Substrates for Single-Molecule Studies of DNA Mismatch Repair - p. 219 16. Chapter Ten: Single-Molecule Insight Into Target Recognition by CRISPR–Cas Complexes - p. 238 17. Chapter Eleven: Preparation of DNA Substrates and Functionalized Glass Surfaces for Correlative Nanomanipulation and Colocalization (NanoCOSM) of Single Molecules - p. 273 18. Chapter Twelve: Measuring Force-Induced Dissociation Kinetics of Protein Complexes Using Single-Molecule Atomic Force Microscopy - p. 296 19. Chapter Thirteen: Improved Force Spectroscopy Using Focused-Ion-Beam-Modified Cantilevers - p. 320 20. Chapter Fourteen: Single-Molecule Characterization of DNA–Protein Interactions Using Nanopore Biosensors - p. 351 21. Chapter Fifteen: Subangstrom Measurements of Enzyme Function Using a Biological Nanopore, SPRNT - p. 385 22. Chapter Sixteen: Multiplexed, Tethered Particle Microscopy for Studies of DNA-Enzyme Dynamics - p. 414 23. Author Index - p. 437 24. Subject Index - p. 459
Single-Molecule Enzymology, Part B, the latest volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in single-molecule enzymology, and includes sections on such topics as force-based and hybrid approaches, fluorescence, high-throughput sm enzymology, and nanopore and tethered particle motion.
1. Cover image - p. iv 2. Title page - p. iv 3. Table of Contents - p. iv 4. Copyright - p. iv 5. Contributors - p. xi 6. Preface - p. xv 7. Chapter One: How to Measure Load-Dependent Kinetics of Individual Motor Molecules Without a Force-Clamp - p. xvi 8. Chapter Two: Studying the Mechanochemistry of Processive Cytoskeletal Motors With an Optical Trap - p. 29 9. Chapter Three: Single-Molecule Optical-Trapping Techniques to Study Molecular Mechanisms of a Replisome - p. 54 10. Chapter Four: Recent Advances in Biological Single-Molecule Applications of Optical Tweezers and Fluorescence Microscopy - p. 84 11. Chapter Five: Direct Visualization of Helicase Dynamics Using Fluorescence Localization and Optical Trapping - p. 119 12. Chapter Six: High-Resolution Optical Tweezers Combined With Single-Molecule Confocal Microscopy - p. 136 13. Chapter Seven: Integrating Optical Tweezers, DNA Tightropes, and Single-Molecule Fluorescence Imaging: Pitfalls and Traps - p. 169 14. Chapter Eight: Single-Stranded DNA Curtains for Studying Homologous Recombination - p. 192 15. Chapter Nine: Inserting Extrahelical Structures into Long DNA Substrates for Single-Molecule Studies of DNA Mismatch Repair - p. 219 16. Chapter Ten: Single-Molecule Insight Into Target Recognition by CRISPR–Cas Complexes - p. 238 17. Chapter Eleven: Preparation of DNA Substrates and Functionalized Glass Surfaces for Correlative Nanomanipulation and Colocalization (NanoCOSM) of Single Molecules - p. 273 18. Chapter Twelve: Measuring Force-Induced Dissociation Kinetics of Protein Complexes Using Single-Molecule Atomic Force Microscopy - p. 296 19. Chapter Thirteen: Improved Force Spectroscopy Using Focused-Ion-Beam-Modified Cantilevers - p. 320 20. Chapter Fourteen: Single-Molecule Characterization of DNA–Protein Interactions Using Nanopore Biosensors - p. 351 21. Chapter Fifteen: Subangstrom Measurements of Enzyme Function Using a Biological Nanopore, SPRNT - p. 385 22. Chapter Sixteen: Multiplexed, Tethered Particle Microscopy for Studies of DNA-Enzyme Dynamics - p. 414 23. Author Index - p. 437 24. Subject Index - p. 459
