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Originally published as the stand-alone Chlamydomonas Sourcebook, then expanded as the second volume in a three-part comprehensive gold-standard reference, The Chlamydomonas Sourcebook: Organellar and Metabolic Processes has been fully revised and updated to include a wealth of new knowledge and resources for the Chlamydomonas community. It details the tremendous progress recently made with respect to imaging the ultrastructure of cells, dissecting acclimation and biosynthetic responses, and elucidating molecular processes underlying the biology of organelles. In particular, this volume includes exciting new developments in the use of imaging technologies for examining supramolecular organization of the chloroplast, defining mechanisms of branched electron transfer pathways in photosynthesis, dissecting the organization of pyrenoids and CO2 concentration mechanisms, presenting the intricacies associated with acclimation to environmental conditions and providing new insights into dark metabolism and the network of fermentative metabolism.

This book thus presents the latest advances in both the research and uses of new experimental approaches and technologies, making this a must-have resource for researchers and students working in plant science and photosynthesis, fertility, mammalian vision, aspects of human disease, acclimation to environmental change, and the biogenesis of cellular complexes.

  • Describes molecular techniques, analysis of the recently sequenced genome, reviews of the current status of the diverse fields in which Chlamydomonas is used as a model organism
  • Provides methods for Chlamydomonas research and best practices for their applications; this includes methods for cell culture, preservation of cultures, preparation of media, lists of inhibitors, and other additives to culture media, classical genetic manipulation, and new approaches for gene transfer and editing technologies
  • Assists researchers with common laboratory problems such as contamination

1. Introduction - p. xxi 2. Historical overview - p. 1 3. The comparative, biochemistry, genetics, and evolution of starch metabolism in Chlamydomonas reinhardtii - p. 23 4. Chlamydomonas glycerolipid metabolism - p. 51 5. Nitrogen metabolism in Chlamydomonas - p. 99 6. Chapter 4. Phosphorus and sulfur uptake, assimilation, and deprivation responses - p. 129 7. Chapter 5. Trace metal nutrition and response to deficiency - p. 167 8. Sensory photoreceptors in Chlamydomonas - p. 205 9. Chapter 7. Rubisco and inorganic carbon assimilation - p. 223 10. Chapter 8. Photoproduction of reducing power and the Calvin-Benson cycle - p. 273 11. Chapter 9. Metabolic networks during dark anoxia - p. 317 12. Chapter 10. Hydrogenases and hydrogen production - p. 343 13. Chapter 11. The mitochondrion: from genome to proteome - p. 369 14. Chapter 12. The chloroplast in a changing environment: from genome to proteome - p. 413 15. Chapter 13. Control of organellar gene expression by nucleus-encoded proteins - p. 443 16. Chapter 14. Translation and protein synthesis in the chloroplast - p. 467 17. Chapter 15. Photosynthesis: light harvesting - p. 509 18. Chapter 16. Photosystems I and II - p. 525 19. Chloroplast ATP synthase and the cytochrome b6f complex - p. 561 20. The multiple routes of photosynthetic electron transfer in Chlamydomonas reinhardtii - p. 591 21. Chapter 19. The assembly of photosynthetic proteins - p. 615 22. Chapter 20. Molecular chaperones, proteases, and unfolded protein responses - p. 647 23. Chapter 21. Tetrapyrrole biosynthesis and signaling (chlorophyll, heme, and bilins) - p. 691 24. Carotenoids in Chlamydomonas - p. 733 25. Chapter 23. Supramolecular organization of chloroplast membranes - p. 763 26. Chapter 24. State transitions - p. 787 27. Chapter 25. Photoprotection - p. 807 28. Index - p. 829

Descripcion
Descripción

Originally published as the stand-alone Chlamydomonas Sourcebook, then expanded as the second volume in a three-part comprehensive gold-standard reference, The Chlamydomonas Sourcebook: Organellar and Metabolic Processes has been fully revised and updated to include a wealth of new knowledge and resources for the Chlamydomonas community. It details the tremendous progress recently made with respect to imaging the ultrastructure of cells, dissecting acclimation and biosynthetic responses, and elucidating molecular processes underlying the biology of organelles. In particular, this volume includes exciting new developments in the use of imaging technologies for examining supramolecular organization of the chloroplast, defining mechanisms of branched electron transfer pathways in photosynthesis, dissecting the organization of pyrenoids and CO2 concentration mechanisms, presenting the intricacies associated with acclimation to environmental conditions and providing new insights into dark metabolism and the network of fermentative metabolism.

This book thus presents the latest advances in both the research and uses of new experimental approaches and technologies, making this a must-have resource for researchers and students working in plant science and photosynthesis, fertility, mammalian vision, aspects of human disease, acclimation to environmental change, and the biogenesis of cellular complexes.

  • Describes molecular techniques, analysis of the recently sequenced genome, reviews of the current status of the diverse fields in which Chlamydomonas is used as a model organism
  • Provides methods for Chlamydomonas research and best practices for their applications; this includes methods for cell culture, preservation of cultures, preparation of media, lists of inhibitors, and other additives to culture media, classical genetic manipulation, and new approaches for gene transfer and editing technologies
  • Assists researchers with common laboratory problems such as contamination

1. Introduction - p. xxi 2. Historical overview - p. 1 3. The comparative, biochemistry, genetics, and evolution of starch metabolism in Chlamydomonas reinhardtii - p. 23 4. Chlamydomonas glycerolipid metabolism - p. 51 5. Nitrogen metabolism in Chlamydomonas - p. 99 6. Chapter 4. Phosphorus and sulfur uptake, assimilation, and deprivation responses - p. 129 7. Chapter 5. Trace metal nutrition and response to deficiency - p. 167 8. Sensory photoreceptors in Chlamydomonas - p. 205 9. Chapter 7. Rubisco and inorganic carbon assimilation - p. 223 10. Chapter 8. Photoproduction of reducing power and the Calvin-Benson cycle - p. 273 11. Chapter 9. Metabolic networks during dark anoxia - p. 317 12. Chapter 10. Hydrogenases and hydrogen production - p. 343 13. Chapter 11. The mitochondrion: from genome to proteome - p. 369 14. Chapter 12. The chloroplast in a changing environment: from genome to proteome - p. 413 15. Chapter 13. Control of organellar gene expression by nucleus-encoded proteins - p. 443 16. Chapter 14. Translation and protein synthesis in the chloroplast - p. 467 17. Chapter 15. Photosynthesis: light harvesting - p. 509 18. Chapter 16. Photosystems I and II - p. 525 19. Chloroplast ATP synthase and the cytochrome b6f complex - p. 561 20. The multiple routes of photosynthetic electron transfer in Chlamydomonas reinhardtii - p. 591 21. Chapter 19. The assembly of photosynthetic proteins - p. 615 22. Chapter 20. Molecular chaperones, proteases, and unfolded protein responses - p. 647 23. Chapter 21. Tetrapyrrole biosynthesis and signaling (chlorophyll, heme, and bilins) - p. 691 24. Carotenoids in Chlamydomonas - p. 733 25. Chapter 23. Supramolecular organization of chloroplast membranes - p. 763 26. Chapter 24. State transitions - p. 787 27. Chapter 25. Photoprotection - p. 807 28. Index - p. 829

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