New discoveries in the field of stem cells increasingly dominate the news and scientific literature revealing an avalanche of new knowledge and research tools that are producing therapies for cancer, heart disease, diabetes, and a wide variety of other diseases that afflict humanity. The Handbook of Stem Cells integrates this exciting area of life science, combining in two volumes the requisites for a general understanding of adult and embryonic stem cells. Organized in two volumes entitled Pluripotent Stem Cells & Cell Biology and Adult & Fetal Stem Cells, this work contains contributions from the world’s experts in stem cell research to provide a description of the tools, methods, and experimental protocols needed to study and characterize stem cells and progenitor populations as well as a the latest information of what is known about each specific organ system.
1. Cover image 2. Title page 1 3. Title page 2 4. Table of Contents 5. Copyright 6. Dedication 7. Foreword 8. Preface 9. Contributors 10. Contributors 11. VOLUME 1: Pluripotent Stem Cells 12. Chapter 1. Why Stem Cell Research? Advances in the Field 13. Chapter 2. “Stemness”: Definitions, Criteria, and Standards 14. Chapter 3. Pluripotential Stem Cells from Vertebrate Embryos: Present Perspective and Future Challenges 15. Chapter 4. Embryonic Stem Cells in Perspective 16. Chapter 5. The Molecular Circuitry Underlying Pluripotency in Embryonic Stem Cells and iPS Cells 17. Chapter 6. Molecular Organization of Cells 18. Chapter 7. Stem Cell Niches 19. Chapter 8. Mechanisms of Stem Cell Self-Renewal 20. Chapter 9. Cell Cycle Regulators in Stem Cells 21. Chapter 10. Cell Fusion and the Differentiated State 22. Chapter 11. How Cells Change Their Phenotype 23. Chapter 12. Somatic Cloning and Epigenetic Reprogramming in Mammals 24. Chapter 13. Engineered Proteins for Controlling Gene Expression 25. Chapter 14. Differentiation in Early Development 26. Chapter 15. Developmental Mechanisms of Regeneration 27. Chapter 16. Primordial Germ Cells in Mouse and Human 28. Chapter 17. Cell–ECM Interactions in Repair and Regeneration 29. Chapter 18. Induced Pluripotent Stem Cells 30. Chapter 19. Feeder-Free and Defined Culture Conditions for hESC Growth 31. Chapter 20. Isolation and Maintenance of Murine Embryonic Stem Cells 32. Chapter 21. Isolation and Maintenance of Primate ES Cells 33. Chapter 22. Embryonic Stem Cells: Derivation and Properties 34. Chapter 23. Approaches for Derivation and Maintenance of Human ES Cells: Detailed Procedures and Alternatives 35. Chapter 24. Alternate Sources of Human Embryonic Stem Cells 36. Chapter 25. Genetic Approaches in Human Embryonic Stem Cells and their Derivatives: Prospects for Regenerative Medicine 37. Chapter 26. Genetic Manipulation of Human Embryonic Stem Cells 38. Chapter 27. Homologous Recombination in Human Embryonic Stem Cells 39. Chapter 28. Derivation and Differentiation of Human Embryonic Germ Cells 40. Chapter 29. Growth Factors and the Serum-Free Culture of Human Pluripotent Stem Cells 41. Chapter 30. Feeder-Free Culture 42. Chapter 31. Surface Antigen Markers 43. Chapter 32. Lineage Marking 44. Chapter 33. Genomic Reprogramming 45. Chapter 34. Microarray Analysis of Stem Cells and Differentiation 46. Chapter 35. Potential of ES Cell Differentiation Culture for Vascular Biology 47. Chapter 36. Use of Embryonic Stem Cells to Treat Heart Disease 48. Chapter 37. Embryonic Stem Cells in Tissue Engineering 49. Chapter 38. Zebrafish and Stem Cell Research 50. VOLUME 2: Adult and Fetal Stem Cells 51. Chapter 39. The Development of Epithelial Stem Cell Concepts 52. Chapter 40. Stem Cells Derived from Amniotic Fluid 53. Chapter 41. Update on “Stem and Progenitor Cells Isolated from Cord Blood” 54. Chapter 42. Stem Cells in Extraembryonic Lineages 55. Chapter 43. Mesenchymal Stem Cells in Regenerative Medicine 56. Chapter 44. Multipotent Adult Progenitor Cells 57. Chapter 45. Mesenchymal Stem Cells 58. Chapter 46. Stem Cells Derived from Fat – an Update 59. Chapter 47. Ontogeny of the Hematopoietic System 60. Chapter 48. Hematopoietic Stem Cells 61. Chapter 49. Cell Therapy for Blood Substitutes 62. Chapter 50. Peripheral Blood Stem Cells 63. Chapter 51. Human Vascular Progenitor Cells 64. Chapter 52. Stem Cells and the Regenerating Heart 65. Chapter 53. Cardiac Stem Cells – Biology and Therapeutic Applications 66. Chapter 54. Stem Cells and Heart Disease 67. Chapter 55. Skeletal Muscle Stem Cells 68. Chapter 56. Stem Cells for the Treatment of Muscular Dystrophy 69. Chapter 57. Myoblast Transplantation in Skeletal Muscles 70. Chapter 58. Neurogenesis in the Vertebrate Embryo 71. Chapter 59. The Nervous System 72. Chapter 60. Stem Cells in the Adult Brain 73. Chapter 61. Neural Stem Cells – Therapeutic Applications in Neurodegenerative Diseases 74. Chapter 62. Spinal Cord Injury 75. Chapter 63. Sensory Epithelium of the Eye and Ear 76. Chapter 64. Epithelial Skin Stem Cells 77. Chapter 65. Regeneration of Epidermis from Adult Human Keratinocyte Stem Cells 78. Chapter 66. Burns and Skin Ulcers 79. Chapter 67. Stem Cells in the Gastrointestinal Tract 80. Chapter 68. Pancreatic Stem Cells 81. Chapter 69. Adult Progenitor Cells as a Potential Treatment for Diabetes 82. Chapter 70. Islet Cell Therapy and Pancreatic Stem Cells 83. Chapter 71. Cell Therapy of Liver Disease: From Hepatocytes to Stem Cells 84. Chapter 72. Adult Liver Stem Cells 85. Chapter 73. Cell Lineages and Stem Cells in the Embryonic Kidney 86. Chapter 74. Orthopaedic Applications of Stem Cells 87. Chapter 75. Somatic Cells – Growth and Expansion Potential of T Lymphocytes 88. Chapter 76. Designing Tunable Artificial Matrices for Stem Cell Culture 89. Chapter 77. Stem Cell Gene Therapy 90. Chapter 78. Histogenesis in Three-Dimensional Scaffolds 91. Chapter 79. Stem Cells in Tissue Engineering 92. Chapter 80. Ethical Considerations 93. Chapter 81. Stem Cell Research – Religious Considerations 94. Chapter 82. US Stem Cell Research Policy 95. Chapter 83. Overview of the FDA Regulatory Process 96. Chapter 84. It’s Not About Curiosity, It’s About Cures: Stem Cell Research – People Help Drive Progress 97. Index
New discoveries in the field of stem cells increasingly dominate the news and scientific literature revealing an avalanche of new knowledge and research tools that are producing therapies for cancer, heart disease, diabetes, and a wide variety of other diseases that afflict humanity. The Handbook of Stem Cells integrates this exciting area of life science, combining in two volumes the requisites for a general understanding of adult and embryonic stem cells. Organized in two volumes entitled Pluripotent Stem Cells & Cell Biology and Adult & Fetal Stem Cells, this work contains contributions from the world’s experts in stem cell research to provide a description of the tools, methods, and experimental protocols needed to study and characterize stem cells and progenitor populations as well as a the latest information of what is known about each specific organ system.
1. Cover image 2. Title page 1 3. Title page 2 4. Table of Contents 5. Copyright 6. Dedication 7. Foreword 8. Preface 9. Contributors 10. Contributors 11. VOLUME 1: Pluripotent Stem Cells 12. Chapter 1. Why Stem Cell Research? Advances in the Field 13. Chapter 2. “Stemness”: Definitions, Criteria, and Standards 14. Chapter 3. Pluripotential Stem Cells from Vertebrate Embryos: Present Perspective and Future Challenges 15. Chapter 4. Embryonic Stem Cells in Perspective 16. Chapter 5. The Molecular Circuitry Underlying Pluripotency in Embryonic Stem Cells and iPS Cells 17. Chapter 6. Molecular Organization of Cells 18. Chapter 7. Stem Cell Niches 19. Chapter 8. Mechanisms of Stem Cell Self-Renewal 20. Chapter 9. Cell Cycle Regulators in Stem Cells 21. Chapter 10. Cell Fusion and the Differentiated State 22. Chapter 11. How Cells Change Their Phenotype 23. Chapter 12. Somatic Cloning and Epigenetic Reprogramming in Mammals 24. Chapter 13. Engineered Proteins for Controlling Gene Expression 25. Chapter 14. Differentiation in Early Development 26. Chapter 15. Developmental Mechanisms of Regeneration 27. Chapter 16. Primordial Germ Cells in Mouse and Human 28. Chapter 17. Cell–ECM Interactions in Repair and Regeneration 29. Chapter 18. Induced Pluripotent Stem Cells 30. Chapter 19. Feeder-Free and Defined Culture Conditions for hESC Growth 31. Chapter 20. Isolation and Maintenance of Murine Embryonic Stem Cells 32. Chapter 21. Isolation and Maintenance of Primate ES Cells 33. Chapter 22. Embryonic Stem Cells: Derivation and Properties 34. Chapter 23. Approaches for Derivation and Maintenance of Human ES Cells: Detailed Procedures and Alternatives 35. Chapter 24. Alternate Sources of Human Embryonic Stem Cells 36. Chapter 25. Genetic Approaches in Human Embryonic Stem Cells and their Derivatives: Prospects for Regenerative Medicine 37. Chapter 26. Genetic Manipulation of Human Embryonic Stem Cells 38. Chapter 27. Homologous Recombination in Human Embryonic Stem Cells 39. Chapter 28. Derivation and Differentiation of Human Embryonic Germ Cells 40. Chapter 29. Growth Factors and the Serum-Free Culture of Human Pluripotent Stem Cells 41. Chapter 30. Feeder-Free Culture 42. Chapter 31. Surface Antigen Markers 43. Chapter 32. Lineage Marking 44. Chapter 33. Genomic Reprogramming 45. Chapter 34. Microarray Analysis of Stem Cells and Differentiation 46. Chapter 35. Potential of ES Cell Differentiation Culture for Vascular Biology 47. Chapter 36. Use of Embryonic Stem Cells to Treat Heart Disease 48. Chapter 37. Embryonic Stem Cells in Tissue Engineering 49. Chapter 38. Zebrafish and Stem Cell Research 50. VOLUME 2: Adult and Fetal Stem Cells 51. Chapter 39. The Development of Epithelial Stem Cell Concepts 52. Chapter 40. Stem Cells Derived from Amniotic Fluid 53. Chapter 41. Update on “Stem and Progenitor Cells Isolated from Cord Blood” 54. Chapter 42. Stem Cells in Extraembryonic Lineages 55. Chapter 43. Mesenchymal Stem Cells in Regenerative Medicine 56. Chapter 44. Multipotent Adult Progenitor Cells 57. Chapter 45. Mesenchymal Stem Cells 58. Chapter 46. Stem Cells Derived from Fat – an Update 59. Chapter 47. Ontogeny of the Hematopoietic System 60. Chapter 48. Hematopoietic Stem Cells 61. Chapter 49. Cell Therapy for Blood Substitutes 62. Chapter 50. Peripheral Blood Stem Cells 63. Chapter 51. Human Vascular Progenitor Cells 64. Chapter 52. Stem Cells and the Regenerating Heart 65. Chapter 53. Cardiac Stem Cells – Biology and Therapeutic Applications 66. Chapter 54. Stem Cells and Heart Disease 67. Chapter 55. Skeletal Muscle Stem Cells 68. Chapter 56. Stem Cells for the Treatment of Muscular Dystrophy 69. Chapter 57. Myoblast Transplantation in Skeletal Muscles 70. Chapter 58. Neurogenesis in the Vertebrate Embryo 71. Chapter 59. The Nervous System 72. Chapter 60. Stem Cells in the Adult Brain 73. Chapter 61. Neural Stem Cells – Therapeutic Applications in Neurodegenerative Diseases 74. Chapter 62. Spinal Cord Injury 75. Chapter 63. Sensory Epithelium of the Eye and Ear 76. Chapter 64. Epithelial Skin Stem Cells 77. Chapter 65. Regeneration of Epidermis from Adult Human Keratinocyte Stem Cells 78. Chapter 66. Burns and Skin Ulcers 79. Chapter 67. Stem Cells in the Gastrointestinal Tract 80. Chapter 68. Pancreatic Stem Cells 81. Chapter 69. Adult Progenitor Cells as a Potential Treatment for Diabetes 82. Chapter 70. Islet Cell Therapy and Pancreatic Stem Cells 83. Chapter 71. Cell Therapy of Liver Disease: From Hepatocytes to Stem Cells 84. Chapter 72. Adult Liver Stem Cells 85. Chapter 73. Cell Lineages and Stem Cells in the Embryonic Kidney 86. Chapter 74. Orthopaedic Applications of Stem Cells 87. Chapter 75. Somatic Cells – Growth and Expansion Potential of T Lymphocytes 88. Chapter 76. Designing Tunable Artificial Matrices for Stem Cell Culture 89. Chapter 77. Stem Cell Gene Therapy 90. Chapter 78. Histogenesis in Three-Dimensional Scaffolds 91. Chapter 79. Stem Cells in Tissue Engineering 92. Chapter 80. Ethical Considerations 93. Chapter 81. Stem Cell Research – Religious Considerations 94. Chapter 82. US Stem Cell Research Policy 95. Chapter 83. Overview of the FDA Regulatory Process 96. Chapter 84. It’s Not About Curiosity, It’s About Cures: Stem Cell Research – People Help Drive Progress 97. Index
