Immuno-oncology and Immunotherapy, Part G, Volume 202 in the Methods in Cell Biology series, highlights new advances in the field, with this new volume presenting interesting chapters on a variety of timely topics, including In situ imaging of pulmonary invariant Natural Killer T cells in precision cut lung slices, Immunofluorescent microscopy to quantify and characterize chromosomal instability, Spatial techniques for understanding the tumor microenvironment, Very high-flex immunophenotyping using co-linear cytofluorometric evaluation, Large-scale CRISPR-Cas9 screens to define regulators of immune checkpoint biology, Assessment of cytoplasmic dsDNA for cellular immunogenicty, and much more.
Additional sections cover Harmonization of a multiparametric flow cytometry panel for immunological monitoring on PBMC and whole blood samples: a real-life example, Quantitative assessment of arterial remodeling from confocal microscopy image analysis of the mid-gestation mouse decidua, Phenotypic characterization and function of tumor infiltrating and peripheral B cells in cancer patients, Methodological Advances to analyze Cancer-Associated Fibroblast-derived ECM effects on Macrophage Polarization, and many other topics that will be of interest to readers.
1. Title of Book - p. i 2. Cover image - p. i 3. Title page - p. ii 4. Table of Contents - p. iii 5. Series Page - p. iii 6. Copyright - p. iv 7. Contributors - p. xv 8. Chapter One: Establishment single lung tumors through orthotopic injection - p. 1 9. Chapter Two: Intracardiac injection as a metastatic model in lung cancer - p. 15 10. Chapter Three: Adeno-associated viral vector administration to the inner ear and phenotype evaluation in a mouse model of hearing loss - p. 25 11. Chapter Four: DiLiCre2.0 mouse model: An advanced genome-editing tool to induce mutagenesis in vivo with high spatio-temporal resolution - p. 41 12. Chapter Five: A new inducible mouse model of FH loss - p. 59 13. Chapter Six: Monitoring neuronal mitophagy and locomotion deficits in a C. elegans model of Alzheimer’s disease - p. 89 14. Chapter Seven: Experimental models and methods for infected wound modeling with Staphylococcus aureus - p. 111 15. Chapter Eight: Multiplex immunohistochemistry (IHC) profiling of murine tumour-infiltrating T cells - p. 129 16. Chapter Nine: Isolation of regulatory T cells from healthy murine mammary glands - p. 149 17. Chapter Ten: Metastasis mouse model of breast cancer derived from circulating tumor cells (CTCs) after primary orthotopic tumor resection - p. 163 18. Chapter Eleven: Establishment of humanized patient-derived xenografts (PDXs) from primary human colorectal cancer tumors - p. 175 19. Chapter Twelve: Modeling stress-induced proteinopathies in Caenorhabditis elegans - p. 201 20. Chapter Thirteen: Intravital imaging of mouse subcutaneous tumors placed in the ear for the study of immune cell interaction with blood vessels - p. 233 21. Chapter Fourteen: Induction of allergic dermatitis with dinitrofluorobenzene in Yama mice - p. 241
Immuno-oncology and Immunotherapy, Part G, Volume 202 in the Methods in Cell Biology series, highlights new advances in the field, with this new volume presenting interesting chapters on a variety of timely topics, including In situ imaging of pulmonary invariant Natural Killer T cells in precision cut lung slices, Immunofluorescent microscopy to quantify and characterize chromosomal instability, Spatial techniques for understanding the tumor microenvironment, Very high-flex immunophenotyping using co-linear cytofluorometric evaluation, Large-scale CRISPR-Cas9 screens to define regulators of immune checkpoint biology, Assessment of cytoplasmic dsDNA for cellular immunogenicty, and much more.
Additional sections cover Harmonization of a multiparametric flow cytometry panel for immunological monitoring on PBMC and whole blood samples: a real-life example, Quantitative assessment of arterial remodeling from confocal microscopy image analysis of the mid-gestation mouse decidua, Phenotypic characterization and function of tumor infiltrating and peripheral B cells in cancer patients, Methodological Advances to analyze Cancer-Associated Fibroblast-derived ECM effects on Macrophage Polarization, and many other topics that will be of interest to readers.
1. Title of Book - p. i 2. Cover image - p. i 3. Title page - p. ii 4. Table of Contents - p. iii 5. Series Page - p. iii 6. Copyright - p. iv 7. Contributors - p. xv 8. Chapter One: Establishment single lung tumors through orthotopic injection - p. 1 9. Chapter Two: Intracardiac injection as a metastatic model in lung cancer - p. 15 10. Chapter Three: Adeno-associated viral vector administration to the inner ear and phenotype evaluation in a mouse model of hearing loss - p. 25 11. Chapter Four: DiLiCre2.0 mouse model: An advanced genome-editing tool to induce mutagenesis in vivo with high spatio-temporal resolution - p. 41 12. Chapter Five: A new inducible mouse model of FH loss - p. 59 13. Chapter Six: Monitoring neuronal mitophagy and locomotion deficits in a C. elegans model of Alzheimer’s disease - p. 89 14. Chapter Seven: Experimental models and methods for infected wound modeling with Staphylococcus aureus - p. 111 15. Chapter Eight: Multiplex immunohistochemistry (IHC) profiling of murine tumour-infiltrating T cells - p. 129 16. Chapter Nine: Isolation of regulatory T cells from healthy murine mammary glands - p. 149 17. Chapter Ten: Metastasis mouse model of breast cancer derived from circulating tumor cells (CTCs) after primary orthotopic tumor resection - p. 163 18. Chapter Eleven: Establishment of humanized patient-derived xenografts (PDXs) from primary human colorectal cancer tumors - p. 175 19. Chapter Twelve: Modeling stress-induced proteinopathies in Caenorhabditis elegans - p. 201 20. Chapter Thirteen: Intravital imaging of mouse subcutaneous tumors placed in the ear for the study of immune cell interaction with blood vessels - p. 233 21. Chapter Fourteen: Induction of allergic dermatitis with dinitrofluorobenzene in Yama mice - p. 241
