In Situ Molecular Pathology and Co-Expression Analyses explains, in easy-to-understand language, simplified ways of understanding and performing in situ hybridization and immunohistochemistry tests. The book also focuses on straightforward protocols used to simultaneously detect two or more proteins/nucleic acids within intact tissue by doing co-expression analyses.
The fields of in situ hybridization and immunohistochemistry have expanded rapidly due to the use of computer-based analysis. To get the most out of these automated platforms, researchers and diagnostic biomedical investigators must have a solid understanding of the basics of in situ-based tests, protocols, and regimens for troubleshooting.
Practicing molecular pathologists, clinical chemists, and toxicologists, as well as clinicians and researchers in training, will benefit from this book's clear presentation of protocols and theoretical framework.
1. 1. Introduction - p. VII 2. 2. The Basics of Molecular Pathology - p. 5 3. 3. The Biochemical Basis of In Situ Hybridization and Immunohistochemistry - p. 37 4. 4. The Basics of In Situ Hybridization - p. 77 5. 5. The Basics of Immunohistochemistry - p. 123 6. 6. The Basics of Histologic Interpretations of Tissues - p. 162 7. 7. The Recommended Protocol for in Situ Hybridization - p. 196 8. 8. The Recommended Protocol for Immunohistochemistry - p. 203 9. 9. Co-Expression Analyses - p. 217 10. 10. PCR In Situ Hybridization and RT In Situ PCR - p. 241 11. Appendix 1. Locked Nucleic Acids—Properties and Applications - p. 260 12. Appendix 2 - p. 267 13. Appendix 3 - p. 281 14. Index - p. 283
In Situ Molecular Pathology and Co-Expression Analyses explains, in easy-to-understand language, simplified ways of understanding and performing in situ hybridization and immunohistochemistry tests. The book also focuses on straightforward protocols used to simultaneously detect two or more proteins/nucleic acids within intact tissue by doing co-expression analyses.
The fields of in situ hybridization and immunohistochemistry have expanded rapidly due to the use of computer-based analysis. To get the most out of these automated platforms, researchers and diagnostic biomedical investigators must have a solid understanding of the basics of in situ-based tests, protocols, and regimens for troubleshooting.
Practicing molecular pathologists, clinical chemists, and toxicologists, as well as clinicians and researchers in training, will benefit from this book's clear presentation of protocols and theoretical framework.
1. 1. Introduction - p. VII 2. 2. The Basics of Molecular Pathology - p. 5 3. 3. The Biochemical Basis of In Situ Hybridization and Immunohistochemistry - p. 37 4. 4. The Basics of In Situ Hybridization - p. 77 5. 5. The Basics of Immunohistochemistry - p. 123 6. 6. The Basics of Histologic Interpretations of Tissues - p. 162 7. 7. The Recommended Protocol for in Situ Hybridization - p. 196 8. 8. The Recommended Protocol for Immunohistochemistry - p. 203 9. 9. Co-Expression Analyses - p. 217 10. 10. PCR In Situ Hybridization and RT In Situ PCR - p. 241 11. Appendix 1. Locked Nucleic Acids—Properties and Applications - p. 260 12. Appendix 2 - p. 267 13. Appendix 3 - p. 281 14. Index - p. 283
