Gain the knowledge and skills you need to succeed as a clinical lab technician! Textbook of Diagnostic Microbiology, 7th Edition uses a reader-friendly "building-block" approach to help you learn the essentials of diagnostic microbiology. Featuring full-color drawings and photos, this text helps you learn to identify significant isolates in the lab and to develop the critical thinking and problem-solving skills necessary to the accurate diagnosis of infectious diseases. Written by noted educators Connie R. Mahon and Donald C. Lehman, this edition adds new content on SARS-CoV-2 and COVID-19, along with the latest information on prevention, treatment modalities, and CDC guidelines.
1. Part 1: Introduction to clinical microbiology - p. x 2. 1. Bacterial cell structure, physiology, metabolism, and genetics - p. 2 3. 2. Host-parasite interaction - p. 25 4. 3. The laboratory role in infection control - p. 50 5. 4. Control of microorganisms: disinfection, sterilization, and microbiology safety - p. 65 6. 5. Performance improvement in the microbiology laboratory - p. 102 7. 6. Specimen collection and processing - p. 123 8. 7. Microscopic examination of materials from infected sites - p. 139 9. 8. Use of colony morphology for the presumptive identification of microorganisms - p. 169 10. 9. Biochemical identification of gram-negative bacteria - p. 183 11. 10. Immunodiagnosis of infectious diseases - p. 199 12. 11. Applications of molecular diagnostics - p. 227 13. 12. Antibacterial mechanisms of action and bacterial resistance mechanisms - p. 250 14. 13. Antimicrobial susceptibility testing - p. 271 15. Part 2: Laboratory identification of significant isolates - p. 305 16. 14. Staphylococcus and similar organisms - p. 308 17. 15. Streptococcus, Enterococcus, and other catalase-negative, gram-positive cocci - p. 324 18. 16. Aerobic gram-positive bacilli - p. 347 19. 17. Neisseria species and Moraxella catarrhalis - p. 371 20. 18. Haemophilus, HACEK group, Legionella, and other fastidious gram-negative bacilli - p. 390 21. 19. Enterobacterales - p. 417 22. Chapter Outline - p. 417 23. Key Terms - p. 417 24. General characteristics - p. 418 25. Opportunistic members of the order Enterobacterales and associated infections - p. 421 26. Primary pathogens in the order Enterobacterales - p. 432 27. Other genera of the order Enterobacterales - p. 439 28. Laboratory diagnosis of the Enterobacterales - p. 439 29. Learning Assessment Questions - p. 452 30. Bibliography - p. 453 31. 20. Vibrio, Aeromonas, Campylobacter, and Campylobacter-like species - p. 455 32. 21. Nonfermenting and miscellaneous gram-negative bacilli - p. 474 33. 22. Anaerobes of clinical importance - p. 496 34. Chapter Outline - p. 496 35. Key Terms - p. 497 36. Important concepts in anaerobic bacteriology - p. 497 37. Frequently encountered anaerobes and their associated diseases - p. 501 38. Specimen selection, collection, transport, and processing - p. 505 39. Procedures for identifying anaerobic isolates - p. 512 40. Antimicrobial susceptibility testing - p. 527 41. Treatment of anaerobe-associated diseases - p. 529 42. Learning assessment questions - p. 530 43. Bibliography - p. 531 44. 23. The spirochetes - p. 533 45. 24. Chlamydia, Rickettsia, and similar organisms - p. 543 46. 25. Mycoplasma and Ureaplasma - p. 558 47. 26. Mycobacterium tuberculosis and nontuberculous mycobacteria - p. 570 48. 27. Medically significant fungi - p. 597 49. 28. Diagnostic parasitology - p. 639 50. Chapter Outline - p. 639 51. Key Terms - p. 639 52. General concepts in parasitology laboratory methods - p. 640 53. Medically important parasitic agents - p. 646 54. Bibliography - p. 706 55. 29. Clinical virology - p. 708 56. Chapter Outline - p. 708 57. Key Terms - p. 708 58. Characteristics of viruses - p. 710 59. Laboratory diagnosis of viral infections - p. 713 60. Double-stranded DNA viruses - p. 718 61. Single-stranded DNA viruses - p. 727 62. Double-stranded RNA viruses - p. 728 63. Single-stranded RNA viruses - p. 729 64. Hepatitis viruses - p. 743 65. Prions - p. 749 66. Treatment and management of viral infections - p. 749 67. Bibliography - p. 751 68. 30. Agents of bioterror and forensic microbiology - p. 753 69. 31. Biofilms: architects of disease - p. 776 70. Part 3: Laboratory diagnosis of infectious diseases: an organ system approach to diagnostic microbiology - p. 789 71. 32. Upper and lower respiratory tract infections - p. 791 72. 33. Skin and soft tissue infections - p. 832 73. 34. Gastrointestinal infections and food poisoning - p. 869 74. 35. Infections of the central nervous system - p. 889 75. 36. Bacteremia and sepsis - p. 904 76. 37. Urinary tract infections - p. 922 77. 38. Genital infections and sexually transmitted infections - p. 942 78. 39. Infections in special populations - p. 974 79. 40. Zoonotic diseases - p. 984 80. 41. Ocular infections - p. 1001 81. Appendix A. Answers to learning assessment questions - p. 1023 82. Appendix B. Selected bacteriologic culture media - p. 1044.e1 83. Appendix C. Selected mycology culture media and stains - p. 1044.e19 84. Appendix D. Selected procedures - p. 1044.e23 85. Glossary - p. 1045 86. Index - p. 1074
Gain the knowledge and skills you need to succeed as a clinical lab technician! Textbook of Diagnostic Microbiology, 7th Edition uses a reader-friendly "building-block" approach to help you learn the essentials of diagnostic microbiology. Featuring full-color drawings and photos, this text helps you learn to identify significant isolates in the lab and to develop the critical thinking and problem-solving skills necessary to the accurate diagnosis of infectious diseases. Written by noted educators Connie R. Mahon and Donald C. Lehman, this edition adds new content on SARS-CoV-2 and COVID-19, along with the latest information on prevention, treatment modalities, and CDC guidelines.
1. Part 1: Introduction to clinical microbiology - p. x 2. 1. Bacterial cell structure, physiology, metabolism, and genetics - p. 2 3. 2. Host-parasite interaction - p. 25 4. 3. The laboratory role in infection control - p. 50 5. 4. Control of microorganisms: disinfection, sterilization, and microbiology safety - p. 65 6. 5. Performance improvement in the microbiology laboratory - p. 102 7. 6. Specimen collection and processing - p. 123 8. 7. Microscopic examination of materials from infected sites - p. 139 9. 8. Use of colony morphology for the presumptive identification of microorganisms - p. 169 10. 9. Biochemical identification of gram-negative bacteria - p. 183 11. 10. Immunodiagnosis of infectious diseases - p. 199 12. 11. Applications of molecular diagnostics - p. 227 13. 12. Antibacterial mechanisms of action and bacterial resistance mechanisms - p. 250 14. 13. Antimicrobial susceptibility testing - p. 271 15. Part 2: Laboratory identification of significant isolates - p. 305 16. 14. Staphylococcus and similar organisms - p. 308 17. 15. Streptococcus, Enterococcus, and other catalase-negative, gram-positive cocci - p. 324 18. 16. Aerobic gram-positive bacilli - p. 347 19. 17. Neisseria species and Moraxella catarrhalis - p. 371 20. 18. Haemophilus, HACEK group, Legionella, and other fastidious gram-negative bacilli - p. 390 21. 19. Enterobacterales - p. 417 22. Chapter Outline - p. 417 23. Key Terms - p. 417 24. General characteristics - p. 418 25. Opportunistic members of the order Enterobacterales and associated infections - p. 421 26. Primary pathogens in the order Enterobacterales - p. 432 27. Other genera of the order Enterobacterales - p. 439 28. Laboratory diagnosis of the Enterobacterales - p. 439 29. Learning Assessment Questions - p. 452 30. Bibliography - p. 453 31. 20. Vibrio, Aeromonas, Campylobacter, and Campylobacter-like species - p. 455 32. 21. Nonfermenting and miscellaneous gram-negative bacilli - p. 474 33. 22. Anaerobes of clinical importance - p. 496 34. Chapter Outline - p. 496 35. Key Terms - p. 497 36. Important concepts in anaerobic bacteriology - p. 497 37. Frequently encountered anaerobes and their associated diseases - p. 501 38. Specimen selection, collection, transport, and processing - p. 505 39. Procedures for identifying anaerobic isolates - p. 512 40. Antimicrobial susceptibility testing - p. 527 41. Treatment of anaerobe-associated diseases - p. 529 42. Learning assessment questions - p. 530 43. Bibliography - p. 531 44. 23. The spirochetes - p. 533 45. 24. Chlamydia, Rickettsia, and similar organisms - p. 543 46. 25. Mycoplasma and Ureaplasma - p. 558 47. 26. Mycobacterium tuberculosis and nontuberculous mycobacteria - p. 570 48. 27. Medically significant fungi - p. 597 49. 28. Diagnostic parasitology - p. 639 50. Chapter Outline - p. 639 51. Key Terms - p. 639 52. General concepts in parasitology laboratory methods - p. 640 53. Medically important parasitic agents - p. 646 54. Bibliography - p. 706 55. 29. Clinical virology - p. 708 56. Chapter Outline - p. 708 57. Key Terms - p. 708 58. Characteristics of viruses - p. 710 59. Laboratory diagnosis of viral infections - p. 713 60. Double-stranded DNA viruses - p. 718 61. Single-stranded DNA viruses - p. 727 62. Double-stranded RNA viruses - p. 728 63. Single-stranded RNA viruses - p. 729 64. Hepatitis viruses - p. 743 65. Prions - p. 749 66. Treatment and management of viral infections - p. 749 67. Bibliography - p. 751 68. 30. Agents of bioterror and forensic microbiology - p. 753 69. 31. Biofilms: architects of disease - p. 776 70. Part 3: Laboratory diagnosis of infectious diseases: an organ system approach to diagnostic microbiology - p. 789 71. 32. Upper and lower respiratory tract infections - p. 791 72. 33. Skin and soft tissue infections - p. 832 73. 34. Gastrointestinal infections and food poisoning - p. 869 74. 35. Infections of the central nervous system - p. 889 75. 36. Bacteremia and sepsis - p. 904 76. 37. Urinary tract infections - p. 922 77. 38. Genital infections and sexually transmitted infections - p. 942 78. 39. Infections in special populations - p. 974 79. 40. Zoonotic diseases - p. 984 80. 41. Ocular infections - p. 1001 81. Appendix A. Answers to learning assessment questions - p. 1023 82. Appendix B. Selected bacteriologic culture media - p. 1044.e1 83. Appendix C. Selected mycology culture media and stains - p. 1044.e19 84. Appendix D. Selected procedures - p. 1044.e23 85. Glossary - p. 1045 86. Index - p. 1074
