Designed for easy use by both beginning and experienced protein crystallographers, the second edition of Practical Protein Crystallography is an essential handbook for any scientist interested in solving a protein structure. The book includes examples of actual experiments and data, electron density maps, and computer methods. This second edition has new material covering CCP4, SHELX, cryocrystallography, MAD and automated fitting.
1. Cover image - p. Cover 2. Title page - p. Cover 3. Table of Contents - p. Cover 4. Copyright - p. Cover 5. FOREWORD TO THE SECOND EDITION - p. Cover 6. PREFACE TO THE SECOND EDITION - p. xiv 7. ACKNOWLEDGMENTS - p. xviii 8. Chapter 1: LABORATORY TECHNIQUES - p. xx 9. Chapter 2: DATA COLLECTION TECHNIQUES - p. 21 10. Chapter 3: COMPUTATIONAL TECHNIQUES - p. 90 11. 3.1 TERMINOLOGY - p. 91 12. 3.2 BASIC COMPUTER TECHNIQUES - p. 101 13. 3.3 DATA REDUCTION AND STATISTICAL ANALYSIS - p. 114 14. 3.4 THE PATTERSON SYNTHESIS - p. 124 15. 3.5 FOURIER TECHNIQUES - p. 135 16. 3.6 ISOMORPHOUS REPLACEMENT PHASING - p. 145 17. 3.7 MOLECULAR REPLACEMENT - p. 162 18. 3.8 NONCRYSTALLOGRAPHIC SYMMETRY - p. 171 19. 3.9 DENSITY MODIFICATION - p. 176 20. 3.10 MULTIWAVELENGTH DATA WITH ANOMALOUS SCATTERING - p. 182 21. 3.11 REFINEMENT OF COORDINATES - p. 193 22. 3.12 ATTING OF MAPS - p. 217 23. 3.13 ANALYSIS OF COORDINATES - p. 265 24. Chapter 4: XtalView TUTORIALS - p. 269 25. Chapter 5: PROTEIN CRYSTALLOGRAPHY COOKBOOK - p. 328 26. Chapter 6: CRYOCRYSTALLOGRAPHY: BASIC THEORY AND METHODS - p. 408 27. Appendix A: Crystallographic Equations in Computer Code - p. 443 28. Appendix B: Useful Web Sites - p. 454 29. INDEX - p. 462 30. Color Plates - p. 462
Designed for easy use by both beginning and experienced protein crystallographers, the second edition of Practical Protein Crystallography is an essential handbook for any scientist interested in solving a protein structure. The book includes examples of actual experiments and data, electron density maps, and computer methods. This second edition has new material covering CCP4, SHELX, cryocrystallography, MAD and automated fitting.
1. Cover image - p. Cover 2. Title page - p. Cover 3. Table of Contents - p. Cover 4. Copyright - p. Cover 5. FOREWORD TO THE SECOND EDITION - p. Cover 6. PREFACE TO THE SECOND EDITION - p. xiv 7. ACKNOWLEDGMENTS - p. xviii 8. Chapter 1: LABORATORY TECHNIQUES - p. xx 9. Chapter 2: DATA COLLECTION TECHNIQUES - p. 21 10. Chapter 3: COMPUTATIONAL TECHNIQUES - p. 90 11. 3.1 TERMINOLOGY - p. 91 12. 3.2 BASIC COMPUTER TECHNIQUES - p. 101 13. 3.3 DATA REDUCTION AND STATISTICAL ANALYSIS - p. 114 14. 3.4 THE PATTERSON SYNTHESIS - p. 124 15. 3.5 FOURIER TECHNIQUES - p. 135 16. 3.6 ISOMORPHOUS REPLACEMENT PHASING - p. 145 17. 3.7 MOLECULAR REPLACEMENT - p. 162 18. 3.8 NONCRYSTALLOGRAPHIC SYMMETRY - p. 171 19. 3.9 DENSITY MODIFICATION - p. 176 20. 3.10 MULTIWAVELENGTH DATA WITH ANOMALOUS SCATTERING - p. 182 21. 3.11 REFINEMENT OF COORDINATES - p. 193 22. 3.12 ATTING OF MAPS - p. 217 23. 3.13 ANALYSIS OF COORDINATES - p. 265 24. Chapter 4: XtalView TUTORIALS - p. 269 25. Chapter 5: PROTEIN CRYSTALLOGRAPHY COOKBOOK - p. 328 26. Chapter 6: CRYOCRYSTALLOGRAPHY: BASIC THEORY AND METHODS - p. 408 27. Appendix A: Crystallographic Equations in Computer Code - p. 443 28. Appendix B: Useful Web Sites - p. 454 29. INDEX - p. 462 30. Color Plates - p. 462
