New Frontiers and Applications of Synthetic Biology presents a collection of chapters from eminent synthetic biologists across the globe who have established experience and expertise working with synthetic biology. This book offers several important areas of synthetic biology which allow us to read and understand easily. It covers the introduction of synthetic biology and design of promoter, new DNA synthesis and sequencing technology, genome assembly, minimal cells, small synthetic RNA, directed evolution, protein engineering, computational tools, de novo synthesis, phage engineering, a sensor for microorganisms, next-generation diagnostic tools, CRISPR-Cas systems, and more.
This book is a good source for not only researchers in designing synthetic biology, but also for researchers, students, synthetic biologists, metabolic engineers, genome engineers, clinicians, industrialists, stakeholders and policymakers interested in harnessing the potential of synthetic biology in many areas.
1. Chapter 1 : An introduction to advanced technologies in synthetic biology - p. Page xiv 2. Chapter 2 : Design and characterization of synthetic promoters - p. Page 6 3. Chapter 3 : Recent development in DNA synthesis technology - p. Page 20 4. Chapter 4 : Advances, challenges, and opportunities in DNA sequencing technology - p. Page 27 5. Chapter 5 : Recent developments in genome design and assembly tools - p. Page 40 6. Chapter 6 : Design, building, and challenges in synthetic genomics - p. Page 57 7. Chapter 7 : Synthetic minimal cells and their applications - p. Page 79 8. Chapter 8 : Small RNA-based systems for sensing and therapeutic applications - p. Page 94 9. Chapter 9 : High-throughput navigation of the sequence space - p. Page 118 10. Chapter 10 : Advances in protein engineering and its application in synthetic biology - p. Page 140 11. Chapter 11 : Computational tools for design of synthetic genetic circuits - p. Page 155 12. Chapter 12 : Computational tools for synthetic gene optimization - p. Page 168 13. Chapter 13 : De novo design and synthesis of biomolecules - p. Page 187 14. Chapter 14 : A retrobiosynthetic approach for production, conversion, sensing, dynamic regulation and degradation of molecules - p. Page 199 15. Chapter 15 : Advances in engineering of bacteriophages for therapeutic applications - p. Page 212 16. Chapter 16 : Design of synthetic biology for the detection of microorganisms - p. Page 226 17. Chapter 17 : Design of synthetic biological devices for detection and targeting human diseases - p. Page 246 18. Chapter 18 : Engineering cell-based therapies - p. Page 265 19. Chapter 19 : Development of next-generation diagnostic tools using synthetic biology - p. Page 281 20. Chapter 20 : Advances and application of CRISPR-Cas systems - p. Page 324 21. Chapter 21 : Synthetic biology for smart drug biosynthesis and delivery - p. Page 343 22. Chapter 22 : Design and applications of self-assembled soft living materials using synthetic biology - p. Page 358 23. Chapter 23 : Synthetic gene circuits for higher-order information processing - p. Page 371 24. Chapter 24 : Cell-free synthetic biology as an emerging biotechnology - p. Page 390 25. Chapter 25 : Recent development and applications of xeno nucleic acids - p. Page 408 26. Chapter 26 : Developments in the use of microfluidics in synthetic biology - p. Page 420 27. Chapter 27 : Implications of synthetic biology research and development: a structured ethical analysis - p. Page 433 28. Index - p. Page 449
New Frontiers and Applications of Synthetic Biology presents a collection of chapters from eminent synthetic biologists across the globe who have established experience and expertise working with synthetic biology. This book offers several important areas of synthetic biology which allow us to read and understand easily. It covers the introduction of synthetic biology and design of promoter, new DNA synthesis and sequencing technology, genome assembly, minimal cells, small synthetic RNA, directed evolution, protein engineering, computational tools, de novo synthesis, phage engineering, a sensor for microorganisms, next-generation diagnostic tools, CRISPR-Cas systems, and more.
This book is a good source for not only researchers in designing synthetic biology, but also for researchers, students, synthetic biologists, metabolic engineers, genome engineers, clinicians, industrialists, stakeholders and policymakers interested in harnessing the potential of synthetic biology in many areas.
1. Chapter 1 : An introduction to advanced technologies in synthetic biology - p. Page xiv 2. Chapter 2 : Design and characterization of synthetic promoters - p. Page 6 3. Chapter 3 : Recent development in DNA synthesis technology - p. Page 20 4. Chapter 4 : Advances, challenges, and opportunities in DNA sequencing technology - p. Page 27 5. Chapter 5 : Recent developments in genome design and assembly tools - p. Page 40 6. Chapter 6 : Design, building, and challenges in synthetic genomics - p. Page 57 7. Chapter 7 : Synthetic minimal cells and their applications - p. Page 79 8. Chapter 8 : Small RNA-based systems for sensing and therapeutic applications - p. Page 94 9. Chapter 9 : High-throughput navigation of the sequence space - p. Page 118 10. Chapter 10 : Advances in protein engineering and its application in synthetic biology - p. Page 140 11. Chapter 11 : Computational tools for design of synthetic genetic circuits - p. Page 155 12. Chapter 12 : Computational tools for synthetic gene optimization - p. Page 168 13. Chapter 13 : De novo design and synthesis of biomolecules - p. Page 187 14. Chapter 14 : A retrobiosynthetic approach for production, conversion, sensing, dynamic regulation and degradation of molecules - p. Page 199 15. Chapter 15 : Advances in engineering of bacteriophages for therapeutic applications - p. Page 212 16. Chapter 16 : Design of synthetic biology for the detection of microorganisms - p. Page 226 17. Chapter 17 : Design of synthetic biological devices for detection and targeting human diseases - p. Page 246 18. Chapter 18 : Engineering cell-based therapies - p. Page 265 19. Chapter 19 : Development of next-generation diagnostic tools using synthetic biology - p. Page 281 20. Chapter 20 : Advances and application of CRISPR-Cas systems - p. Page 324 21. Chapter 21 : Synthetic biology for smart drug biosynthesis and delivery - p. Page 343 22. Chapter 22 : Design and applications of self-assembled soft living materials using synthetic biology - p. Page 358 23. Chapter 23 : Synthetic gene circuits for higher-order information processing - p. Page 371 24. Chapter 24 : Cell-free synthetic biology as an emerging biotechnology - p. Page 390 25. Chapter 25 : Recent development and applications of xeno nucleic acids - p. Page 408 26. Chapter 26 : Developments in the use of microfluidics in synthetic biology - p. Page 420 27. Chapter 27 : Implications of synthetic biology research and development: a structured ethical analysis - p. Page 433 28. Index - p. Page 449
