Marine enzymes and specialized metabolism - Part B, Volume 605 in the Methods in Enzymology series, highlights experimental methods on diverse marine enzymes involved in the construction of bioactive natural product molecules. Unique sections in this new release include discussions on polysaccharide-degrading enzymes from marine gastropods, radical SAM epimerases from sponge microbes, DMS/P demethylase in bacteria, reconstitution of particulate methane monooxygenase into membrane mimetics, the structure and function of cyanobactin enzymes, marine cyanobacterial polyketide beta-branching enzymology, marine cyanobacterial PKS-NRPS enzymology and structural biology, biochemical profiling of DMSP lyases, and more.
1. Cover image - p. iv 2. Title page - p. iv 3. Table of Contents - p. iv 4. Copyright - p. iv 5. Contributors - p. xi 6. Preface - p. xv 7. Section I: Biosynthesis of Marine Lipids, Polyunsaturated Fatty Acids and Oxylipins - p. xvi 8. Chapter One: Characterization and Application of Marine Microbial Omega-3 Polyunsaturated Fatty Acid Synthesis - p. xvi 9. Chapter Two: Expression of an 8R-Lipoxygenase From the Coral Plexaura homomalla - p. 32 10. Chapter Three: Catalase-Related Allene Oxide Synthase, on a Biosynthetic Route to Fatty Acid Cyclopentenones: Expression and Assay of the Enzyme and Preparation of the 8R-HPETE Substrate - p. 49 11. Chapter Four: Lipoxygenases and Lipoxygenase Products in Marine Diatoms - p. 68 12. Chapter Five: Sterol Sulfates and Sulfotransferases in Marine Diatoms - p. 100 13. Section II: Marine Halogenating and Dehalogenating Enzymes - p. 138 14. Chapter Six: Marine Vanadium-Dependent Haloperoxidases, Their Isolation, Characterization, and Application - p. 138 15. Chapter Seven: Haloalkane Dehalogenases From Marine Organisms - p. 201 16. Chapter Eight: Preparation and Characterization of Tetrabromopyrrole Debrominase From Marine Proteobacteria - p. 251 17. Section III: Biosynthesis and Catabolism of Small Organic Molecules Common in the Marine Environment - p. 265 18. Chapter Nine: Biochemical Profiling of DMSP Lyases - p. 265 19. Chapter Ten: Isolation and Assays of Bacterial Dimethylsulfoniopropionate Lyases - p. 289 20. Chapter Eleven: Assay and Analysis of Dimethylsulfoniopropionate Demethylase - p. 323 21. Chapter Twelve: Recent Advances in the Genetic Manipulation of Methylosinus trichosporium OB3b - p. 333 22. Chapter Thirteen: Methylphosphonic Acid Biosynthesis and Catabolism in Pelagic Archaea and Bacteria - p. 349 23. Section IV: Marine Enzymes, Biotechnology and Physiology - p. 426 24. Chapter Fourteen: Silicatein: A Unique Silica-Synthesizing Catalytic Triad Hydrolase From Marine Sponge Skeletons and Its Multiple Applications - p. 426 25. Chapter Fifteen: Polysaccharide-Degrading Enzymes From Marine Gastropods - p. 455 26. Chapter Sixteen: Characterization of PL-7 Family Alginate Lyases From Marine Organisms and Their Applications - p. 497 27. Chapter Seventeen: Molecular, Enzymatic, and Cellular Characterization of Soluble Adenylyl Cyclase From Aquatic Animals - p. 524
Marine enzymes and specialized metabolism - Part B, Volume 605 in the Methods in Enzymology series, highlights experimental methods on diverse marine enzymes involved in the construction of bioactive natural product molecules. Unique sections in this new release include discussions on polysaccharide-degrading enzymes from marine gastropods, radical SAM epimerases from sponge microbes, DMS/P demethylase in bacteria, reconstitution of particulate methane monooxygenase into membrane mimetics, the structure and function of cyanobactin enzymes, marine cyanobacterial polyketide beta-branching enzymology, marine cyanobacterial PKS-NRPS enzymology and structural biology, biochemical profiling of DMSP lyases, and more.
1. Cover image - p. iv 2. Title page - p. iv 3. Table of Contents - p. iv 4. Copyright - p. iv 5. Contributors - p. xi 6. Preface - p. xv 7. Section I: Biosynthesis of Marine Lipids, Polyunsaturated Fatty Acids and Oxylipins - p. xvi 8. Chapter One: Characterization and Application of Marine Microbial Omega-3 Polyunsaturated Fatty Acid Synthesis - p. xvi 9. Chapter Two: Expression of an 8R-Lipoxygenase From the Coral Plexaura homomalla - p. 32 10. Chapter Three: Catalase-Related Allene Oxide Synthase, on a Biosynthetic Route to Fatty Acid Cyclopentenones: Expression and Assay of the Enzyme and Preparation of the 8R-HPETE Substrate - p. 49 11. Chapter Four: Lipoxygenases and Lipoxygenase Products in Marine Diatoms - p. 68 12. Chapter Five: Sterol Sulfates and Sulfotransferases in Marine Diatoms - p. 100 13. Section II: Marine Halogenating and Dehalogenating Enzymes - p. 138 14. Chapter Six: Marine Vanadium-Dependent Haloperoxidases, Their Isolation, Characterization, and Application - p. 138 15. Chapter Seven: Haloalkane Dehalogenases From Marine Organisms - p. 201 16. Chapter Eight: Preparation and Characterization of Tetrabromopyrrole Debrominase From Marine Proteobacteria - p. 251 17. Section III: Biosynthesis and Catabolism of Small Organic Molecules Common in the Marine Environment - p. 265 18. Chapter Nine: Biochemical Profiling of DMSP Lyases - p. 265 19. Chapter Ten: Isolation and Assays of Bacterial Dimethylsulfoniopropionate Lyases - p. 289 20. Chapter Eleven: Assay and Analysis of Dimethylsulfoniopropionate Demethylase - p. 323 21. Chapter Twelve: Recent Advances in the Genetic Manipulation of Methylosinus trichosporium OB3b - p. 333 22. Chapter Thirteen: Methylphosphonic Acid Biosynthesis and Catabolism in Pelagic Archaea and Bacteria - p. 349 23. Section IV: Marine Enzymes, Biotechnology and Physiology - p. 426 24. Chapter Fourteen: Silicatein: A Unique Silica-Synthesizing Catalytic Triad Hydrolase From Marine Sponge Skeletons and Its Multiple Applications - p. 426 25. Chapter Fifteen: Polysaccharide-Degrading Enzymes From Marine Gastropods - p. 455 26. Chapter Sixteen: Characterization of PL-7 Family Alginate Lyases From Marine Organisms and Their Applications - p. 497 27. Chapter Seventeen: Molecular, Enzymatic, and Cellular Characterization of Soluble Adenylyl Cyclase From Aquatic Animals - p. 524
