Lipids and Membranes: Metabolism, Lipidation, and Lipid-Protein Interactions, Volume 729 provides a broad snapshot of state-of-the-art methods for studying biological lipids and biomembranes. Sections in this new release cover De novo chemoenzymatic construction of lipid membranes, Photoaffinity labeling for cholesterol metabolism, Expression and Characterization of Phospholipase Cb and Ce enzymes, Assembling Retromer-coated membrane tubules for in vitro structure-function studies, Quantitative analysis of S-acylation, Inhibition of palmitoyltransferases, Exploiting protein lipidation to create peptide-based biomaterials, and much more.
Additional sections discuss Optogenetic control of lipid-modifying enzyme activity using LOV domain insertions, Chemoproteomics approaches for inhibition of diacylglycerol kinases, Inhibition of sterol transfer protein function, Preparation of K-Ras4B containing synthetic modifications via expressed protein ligation, Regulation of lipin enzymes on membranes, Use of novel synthetic analogues to decipher the interactions of phosphatidic acids species, Analyzing lipid-nuclear receptor interactions, and much more.
1. Title of Book - p. i 2. Cover image - p. i 3. Title page - p. ii 4. Table of Contents - p. iii 5. Series Page - p. iii 6. Copyright - p. iv 7. Contributors - p. xvii 8. Chapter One: De novo chemoenzymatic construction of lipid membranes - p. 1 9. Chapter Two: Photoaffinity strategies to profile cholesterol metabolite interactomes and binding sites - p. 23 10. Chapter Three: Expression and characterization of phospholipase Cβ and ε enzymes - p. 65 11. Chapter Four: Assembling Retromer-coated membrane tubules for biochemical and structural studies - p. 101 12. Chapter Five: Quantitative analysis of S-acylation - p. 117 13. Chapter Six: Detecting ZDHHC acyltransferase inhibition using acylation-coupled lipophilic induction of polarization (Acyl-cLIP) or LC-MS - p. 129 14. Chapter Seven: One-pot biosynthesis of genetically encoded amphiphiles via protein prenylation - p. 143 15. Chapter Eight: Creation of an optogenetic membrane editor with ultralow background activity - p. 169 16. Chapter Nine: Inhibition of sterol transport protein function - p. 183 17. Chapter Ten: Preparation of K-Ras4B containing synthetic modifications via expressed protein ligation - p. 211 18. Chapter Eleven: A fluorescence and HPLC-based activity assay system for lipid-modifying enzymes: Lipin/Pah phosphatidic acid phosphatase as an optimized example - p. 235 19. Chapter Twelve: Use of novel synthetic analogs to decipher the interactome of phosphatidic acid species in neurosecretory cells - p. 253 20. Chapter Thirteen: Expression and purification of Steroidogenic Factor-1 (NR5A1) nuclear receptor ligand binding domain complexed with the signaling phosphoinositide lipid PI(3,4,5)P3 - p. 277 21. Chapter Fourteen: Reconstitution strategies for exploring interactions of Ebola virus matrix protein with host membrane mimetics - p. 289 22. Chapter Fifteen: Palmitoylation-dependent probes for labeling the Golgi apparatus - p. 305 23. Chapter Sixteen: Real-time measurement of the ATG8 lipidation reaction by fluorescence spectroscopy - p. 327 24. Chapter Seventeen: Optogenetic phospholipase C for measuring phosphoinositide turnover and DAG generation - p. 347 25. Chapter Eighteen: Pharmacological modulation of lipid signaling - p. 369
Lipids and Membranes: Metabolism, Lipidation, and Lipid-Protein Interactions, Volume 729 provides a broad snapshot of state-of-the-art methods for studying biological lipids and biomembranes. Sections in this new release cover De novo chemoenzymatic construction of lipid membranes, Photoaffinity labeling for cholesterol metabolism, Expression and Characterization of Phospholipase Cb and Ce enzymes, Assembling Retromer-coated membrane tubules for in vitro structure-function studies, Quantitative analysis of S-acylation, Inhibition of palmitoyltransferases, Exploiting protein lipidation to create peptide-based biomaterials, and much more.
Additional sections discuss Optogenetic control of lipid-modifying enzyme activity using LOV domain insertions, Chemoproteomics approaches for inhibition of diacylglycerol kinases, Inhibition of sterol transfer protein function, Preparation of K-Ras4B containing synthetic modifications via expressed protein ligation, Regulation of lipin enzymes on membranes, Use of novel synthetic analogues to decipher the interactions of phosphatidic acids species, Analyzing lipid-nuclear receptor interactions, and much more.
1. Title of Book - p. i 2. Cover image - p. i 3. Title page - p. ii 4. Table of Contents - p. iii 5. Series Page - p. iii 6. Copyright - p. iv 7. Contributors - p. xvii 8. Chapter One: De novo chemoenzymatic construction of lipid membranes - p. 1 9. Chapter Two: Photoaffinity strategies to profile cholesterol metabolite interactomes and binding sites - p. 23 10. Chapter Three: Expression and characterization of phospholipase Cβ and ε enzymes - p. 65 11. Chapter Four: Assembling Retromer-coated membrane tubules for biochemical and structural studies - p. 101 12. Chapter Five: Quantitative analysis of S-acylation - p. 117 13. Chapter Six: Detecting ZDHHC acyltransferase inhibition using acylation-coupled lipophilic induction of polarization (Acyl-cLIP) or LC-MS - p. 129 14. Chapter Seven: One-pot biosynthesis of genetically encoded amphiphiles via protein prenylation - p. 143 15. Chapter Eight: Creation of an optogenetic membrane editor with ultralow background activity - p. 169 16. Chapter Nine: Inhibition of sterol transport protein function - p. 183 17. Chapter Ten: Preparation of K-Ras4B containing synthetic modifications via expressed protein ligation - p. 211 18. Chapter Eleven: A fluorescence and HPLC-based activity assay system for lipid-modifying enzymes: Lipin/Pah phosphatidic acid phosphatase as an optimized example - p. 235 19. Chapter Twelve: Use of novel synthetic analogs to decipher the interactome of phosphatidic acid species in neurosecretory cells - p. 253 20. Chapter Thirteen: Expression and purification of Steroidogenic Factor-1 (NR5A1) nuclear receptor ligand binding domain complexed with the signaling phosphoinositide lipid PI(3,4,5)P3 - p. 277 21. Chapter Fourteen: Reconstitution strategies for exploring interactions of Ebola virus matrix protein with host membrane mimetics - p. 289 22. Chapter Fifteen: Palmitoylation-dependent probes for labeling the Golgi apparatus - p. 305 23. Chapter Sixteen: Real-time measurement of the ATG8 lipidation reaction by fluorescence spectroscopy - p. 327 24. Chapter Seventeen: Optogenetic phospholipase C for measuring phosphoinositide turnover and DAG generation - p. 347 25. Chapter Eighteen: Pharmacological modulation of lipid signaling - p. 369
